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Literature summary extracted from
- Linalool isomerase, a membrane-anchored enzyme in the anaerobic monoterpene degradation in Thauera linaloolentis 47Lol (2016), BMC Biochem., 17, 006 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.127 | additional information | enzyme activity requires a reductant for activation, influence of different reducing agents on Lis activity, overview | Thauera linaloolentis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.127 | gene lis, recombinant expression in Escherichia coli strain BL21(DE3) in membranes | Thauera linaloolentis |
| 5.4.4.8 | expression in Escherichia coli | Thauera linaloolentis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.1.127 | additional information | expression of a N-terminally truncated version of the linalool isomerase, representing the cytosolic part of the enzyme only, yields a soluble protein that does not show activity | Thauera linaloolentis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.127 | additional information | detergent-mediated release of the protein from the membrane causes irreversible inhibitory effects on the enzyme activity | Thauera linaloolentis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.127 | additional information | - |
additional information | Michaelis-Menten-kinetics | Thauera linaloolentis | |
| 5.4.4.8 | 0.455 | - |
geraniol | pH 8.0, 35°C | Thauera linaloolentis |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.2.1.127 | inner membrane | membrane-anchored enzyme | Thauera linaloolentis | - |
- |
| 5.4.4.8 | membrane | inner membrane. Protein is predicted to have four transmembrane helices at the N-terminal domain and a cytosolic domain | Thauera linaloolentis | 16020 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.127 | 600000 | - |
gel filtration | Thauera linaloolentis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.127 | (R,S)-linalool | Thauera linaloolentis | - |
beta-myrcene + H2O | - |
r | |
| 4.2.1.127 | (R,S)-linalool | Thauera linaloolentis 47Lol | - |
beta-myrcene + H2O | - |
r | |
| 5.4.4.8 | (R)-linalool | Thauera linaloolentis | - |
geraniol | - |
r | |
| 5.4.4.8 | (R)-linalool | Thauera linaloolentis DSM 12138 | - |
geraniol | - |
r | |
| 5.4.4.8 | (S)-linalool | Thauera linaloolentis | - |
geraniol | - |
r | |
| 5.4.4.8 | (S)-linalool | Thauera linaloolentis DSM 12138 | - |
geraniol | - |
r | |
| 5.4.4.8 | geraniol | Thauera linaloolentis | - |
(R)-linalool + (S)-linalool | - |
r | |
| 5.4.4.8 | geraniol | Thauera linaloolentis DSM 12138 | - |
(R)-linalool + (S)-linalool | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.127 | Thauera linaloolentis | - |
- |
- |
| 4.2.1.127 | Thauera linaloolentis 47Lol | - |
- |
- |
| 5.4.4.8 | Thauera linaloolentis | N6Z5E2 | - |
- |
| 5.4.4.8 | Thauera linaloolentis DSM 12138 | N6Z5E2 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.127 | native enzyme from the inner membrane using subcellular fractionation including a sucrose-gradient centrifugation and ultracentrifugation at 150000 x g, anion exchange and hydrophobic interaction chromatography, and gel filtration, the detergent-mediated release of the protein from the membrane has irreversible inhibitory effects on the enzyme activity, spheroplast preparation | Thauera linaloolentis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.4.8 | 57 | - |
pH 8.0, 35°C | Thauera linaloolentis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.127 | (R,S)-linalool | - |
Thauera linaloolentis | beta-myrcene + H2O | - |
r | |
| 4.2.1.127 | (R,S)-linalool | the enzyme is not stereospecific | Thauera linaloolentis | beta-myrcene + H2O | - |
r | |
| 4.2.1.127 | (R,S)-linalool | - |
Thauera linaloolentis 47Lol | beta-myrcene + H2O | - |
r | |
| 4.2.1.127 | (R,S)-linalool | the enzyme is not stereospecific | Thauera linaloolentis 47Lol | beta-myrcene + H2O | - |
r | |
| 4.2.1.127 | additional information | a bifunctional linalool dehydratase/isomerase that exhibits dehydratase activity, Ec 4.2.1.127, and isomerase activity, EC 5.4.4.4. The enzyme catalyzes the reversible hydration of beta-myrcene to linalool and its isomerization to geraniol. No activity is measured with nerol or citronellol alone. Linalool isomerase activity drops to approx. 50% in the presence of nerol. Activity in the presence of citronellol and geraniol is barely detectable. The enzyme is regioselective and seems to bind nerol with a similar affinity as geraniol, whereas citronellol which lacks the C2-C3 double bond is stronger bound than geraniol. Stereoselectivity analysis | Thauera linaloolentis | ? | - |
? | |
| 4.2.1.127 | additional information | a bifunctional linalool dehydratase/isomerase that exhibits dehydratase activity, Ec 4.2.1.127, and isomerase activity, EC 5.4.4.4. The enzyme catalyzes the reversible hydration of beta-myrcene to linalool and its isomerization to geraniol. No activity is measured with nerol or citronellol alone. Linalool isomerase activity drops to approx. 50% in the presence of nerol. Activity in the presence of citronellol and geraniol is barely detectable. The enzyme is regioselective and seems to bind nerol with a similar affinity as geraniol, whereas citronellol which lacks the C2-C3 double bond is stronger bound than geraniol. Stereoselectivity analysis | Thauera linaloolentis 47Lol | ? | - |
? | |
| 5.4.4.8 | (R)-linalool | - |
Thauera linaloolentis | geraniol | - |
r | |
| 5.4.4.8 | (R)-linalool | - |
Thauera linaloolentis DSM 12138 | geraniol | - |
r | |
| 5.4.4.8 | (S)-linalool | - |
Thauera linaloolentis | geraniol | - |
r | |
| 5.4.4.8 | (S)-linalool | - |
Thauera linaloolentis DSM 12138 | geraniol | - |
r | |
| 5.4.4.8 | geraniol | - |
Thauera linaloolentis | (R)-linalool + (S)-linalool | - |
r | |
| 5.4.4.8 | geraniol | - |
Thauera linaloolentis DSM 12138 | (R)-linalool + (S)-linalool | - |
r | |
| 5.4.4.8 | additional information | presence of a reductant is required, maximum activity in presence of 4 mM dithionite | Thauera linaloolentis | ? | - |
? | |
| 5.4.4.8 | additional information | presence of a reductant is required, maximum activity in presence of 4 mM dithionite | Thauera linaloolentis DSM 12138 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.127 | decamer | 10 x 60000, recombinant enzyme, SDS-PAGE, 10 x 71800, about, sequence calculation | Thauera linaloolentis |
| 5.4.4.8 | ? | x * 71800, calculated, x * 60000-70000, SDS-PAGE | Thauera linaloolentis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.127 | linalool dehydratase/isomerase | - |
Thauera linaloolentis |
| 4.2.1.127 | LIS | - |
Thauera linaloolentis |
| 4.2.1.127 | additional information | cf. EC 5.4.4.4 | Thauera linaloolentis |
| 5.4.4.8 | C666_11245 | - |
Thauera linaloolentis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.127 | 28 | - |
assay at | Thauera linaloolentis |
| 5.4.4.8 | 35 | - |
- |
Thauera linaloolentis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.127 | 8 | - |
assay at | Thauera linaloolentis |
| 5.4.4.8 | 8 | - |
- |
Thauera linaloolentis |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 5.4.4.8 | Thauera linaloolentis | calculated | - |
6.1 |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.4.4.8 | physiological function | strain is able to use the tertiary monoterpene alcohol (R,S)-linalool as sole carbon and energy source under denitrifying conditions | Thauera linaloolentis |
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