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Literature summary extracted from

  • Martinez-Morales, F.; Bertrand, B.; Pasion Nava, A.A.; Tinoco, R.; Acosta-Urdapilleta, L.; Trejo-Hernandez, M.R.
    Production, purification and biochemical characterization of two laccase isoforms produced by Trametes versicolor grown on oak sawdust (2015), Biotechnol. Lett., 37, 391-396 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.10.3.2 environmental protection the enzyme has potential for application in the treatment of contaminated water with low pH values and high phenolic content Trametes versicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 10
-
 2,6-dimethoxyphenol pH 3.0, 23°C Trametes versicolor
1.10.3.2 12
-
 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 23°C Trametes versicolor
1.10.3.2 15
-
 guaiacol pH 5.0, 23°C Trametes versicolor
1.10.3.2 17
-
 syringaldazine pH 5.0, 23°C Trametes versicolor
1.10.3.2 39
-
 syringaldazine pH 5.0, 23°C Trametes versicolor
1.10.3.2 45
-
 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 23°C Trametes versicolor
1.10.3.2 47
-
 2,6-dimethoxyphenol pH 3.0, 23°C Trametes versicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 10000
-
 gel filtration Trametes versicolor
1.10.3.2 60000
-
 gel filtration Trametes versicolor

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Trametes versicolor Q12718
-
-
1.10.3.2 Trametes versicolor Q5IR80
-
-
1.10.3.2 Trametes versicolor HEMIM-9 Q12718
-
-
1.10.3.2 Trametes versicolor HEMIM-9 Q5IR80
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Trametes versicolor

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 mycelium
-
 Trametes versicolor
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Trametes versicolor ?
-
 ?
1.10.3.2 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Trametes versicolor HEMIM-9 ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Trametes versicolor ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Trametes versicolor HEMIM-9 ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Trametes versicolor ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Trametes versicolor HEMIM-9 ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Trametes versicolor ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Trametes versicolor HEMIM-9 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 heterodimer 1 * 55400 + 1 * 66300, SDS-PAGE Trametes versicolor

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 lcc1
-
 Trametes versicolor
1.10.3.2 Lcc2
-
 Trametes versicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 23
-
 assay at Trametes versicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3
-
 substrate: 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) or 2,6-dimethoxyphenol Trametes versicolor
1.10.3.2 3
-
 substrate: 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonix acid) or 2,6-dimethoxyphenol Trametes versicolor
1.10.3.2 5
-
 substrate: guaiacol or syringaldazine Trametes versicolor