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Literature summary extracted from

  • Khan, S.; Adhikari, D.K.; Gupta, S.; Gupta, N.
    High-level expression, purification and characterization of carbazole dioxygenase, a three components dioxygenase, of Pseudomonas GBS.5 (2015), Biotechnol. Lett., 37, 1945-1952 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.12.22 Ferredoxin indispensable for catalytic activity Pseudomonas sp. GBS.5

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.22 expressed in Escherichia coli BL21(DE3) cells Pseudomonas sp. GBS.5

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.22 9H-carbazole + NADH + H+ + O2 Pseudomonas sp. GBS.5
-
2'-aminobiphenyl-2,3-diol + NAD+
-
?
1.14.12.22 9H-carbazole + NADPH + H+ + O2 Pseudomonas sp. GBS.5
-
2'-aminobiphenyl-2,3-diol + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.22 Pseudomonas sp. GBS.5
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.22 glutathione bead chromatography Pseudomonas sp. GBS.5

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.22 9H-carbazole + NADH + H+ + O2
-
Pseudomonas sp. GBS.5 2'-aminobiphenyl-2,3-diol + NAD+
-
?
1.14.12.22 9H-carbazole + NADPH + H+ + O2
-
Pseudomonas sp. GBS.5 2'-aminobiphenyl-2,3-diol + NADP+
-
?
1.14.12.22 anthracene + NADH + H+ + O2
-
Pseudomonas sp. GBS.5 ?
-
?
1.14.12.22 dibenzothiophene + NADH + H+ + O2
-
Pseudomonas sp. GBS.5 ?
-
?
1.14.12.22 fluoranthene + NADH + H+ + O2
-
Pseudomonas sp. GBS.5 ?
-
?
1.14.12.22 additional information the enzyme does not degrade alkanes like pentadecane, hexadecane, heptadecane, octadecane, nonadecane and docosane Pseudomonas sp. GBS.5 ?
-
?
1.14.12.22 phenanthrene + NADH + H+ + O2
-
Pseudomonas sp. GBS.5 ?
-
?
1.14.12.22 pyrene + NADH + H+ + O2
-
Pseudomonas sp. GBS.5 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.22 CarAa
-
Pseudomonas sp. GBS.5
1.14.12.22 carbazole dioxygenase
-
Pseudomonas sp. GBS.5
1.14.12.22 CARDO
-
Pseudomonas sp. GBS.5

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.22 NADH
-
Pseudomonas sp. GBS.5
1.14.12.22 NADPH
-
Pseudomonas sp. GBS.5