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Literature summary extracted from

  • Kars, G.; Alparslan, U.e.
    Heterologous expression of glutamyl-tRNA reductase gene in Rhodobacter sphaeroides O.U.001 to enhance 5-aminolevulinic acid production (2014), Biotechnol. Biotechnol. Equip., 28, 1034-1041 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.70 recombinant enzyme expression in Rhodobacter sphaeroides strain DSM 5864, which synthesizes 5-aminolevulinic acid through the C-4 pathway and also has the genes coding for the enzymes of the C-5 pathway, except for glutamyl-tRNA reductase. Expression of the glutamyl-tRNA reductase gene from Rhodospirillum rubrum enabled the C-5 pathway in Rhodobacter sphaeroides to function upon assembling all the required genes Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.70 L-glutamate 1-semialdehyde + NADP+ + tRNAGlu Rhodospirillum rubrum
-
 L-glutamyl-tRNAGlu + NADPH + H+
-
 ?
1.2.1.70 L-glutamate 1-semialdehyde + NADP+ + tRNAGlu Rhodospirillum rubrum DSM 467
-
 L-glutamyl-tRNAGlu + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.70 Rhodospirillum rubrum
-
-
-
1.2.1.70 Rhodospirillum rubrum DSM 467
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.70 L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
 Rhodospirillum rubrum L-glutamyl-tRNAGlu + NADPH + H+
-
 ?
1.2.1.70 L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
 Rhodospirillum rubrum DSM 467 L-glutamyl-tRNAGlu + NADPH + H+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.70 NADP+
-
 Rhodospirillum rubrum

General Information

EC Number General Information Comment Organism
1.2.1.70 physiological function the enzyme is required for the biosynthesis of 5-aminolevulinic acid Rhodospirillum rubrum