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Literature summary extracted from
- Heterologous production of clavulanic acid intermediates in Streptomyces venezuelae (2017), Biotechnol. Bioprocess Eng., 22, 359-365 .
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.14.11.21 | synthesis | clavulanic acid intermediates: deoxygaunidinoproclavaminic acid, guanidinoproclavaminic acid, and dihydroclavaminic acid are heterologously produced in Streptomyces venezuelae recombinant using four sets of early genes from the clavulanic acid biosynthetic pathway | Streptomyces clavuligerus |
| 2.5.1.66 | synthesis | clavulanic acid intermediates: deoxygaunidinoproclavaminic acid, guanidinoproclavaminic acid, and dihydroclavaminic acid are heterologously produced in Streptomyces venezuelae recombinant using four sets of early genes from the clavulanic acid biosynthetic pathway | Streptomyces clavuligerus |
| 2.5.1.66 | synthesis | synthesis of clavulanic acid intermediates in Streptomyces venezuelae by expression of clavulanic acid biosynthesis genes carboxyethylarginine synthase (Ceas2), beta-lactam synthetase (Bls2), clavaminate synthase (Cas2), and proclavaminate amidinohydrolase (Pah2). The recombinant strain produces deoxygaunidinoproclavaminic acid, guanidinoproclavaminic acid, and dihydroclavaminic acid | Streptomyces clavuligerus |
| 3.5.3.22 | synthesis | clavulanic acid intermediates: deoxygaunidinoproclavaminic acid, guanidinoproclavaminic acid, and dihydroclavaminic acid are heterologously produced in Streptomyces venezuelae recombinant using four sets of early genes from the clavulanic acid biosynthetic pathway | Streptomyces clavuligerus |
| 3.5.3.22 | synthesis | expression of four structural clavulanic acid biosynthesis genes, which encode carboxyethylarginine synthase (Ceas2), beta-lactam synthetase (Bls2), clavaminate synthase (Cas2), and proclavaminate amidinohydrolase (Pah2), in Streptomyces venezuelae enables production of clavulanic acid intermediates deoxygaunidinoproclavaminic acid, guanidinoproclavaminic acid, and dihydroclavaminic acid | Streptomyces clavuligerus |
| 6.3.3.4 | synthesis | clavulanic acid intermediates: deoxygaunidinoproclavaminic acid, guanidinoproclavaminic acid, and dihydroclavaminic acid are heterologously produced in Streptomyces venezuelae recombinant using four sets of early genes from the clavulanic acid biosynthetic pathway | Streptomyces clavuligerus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.11.21 | Streptomyces venezuelae YJ028 is utilized as the heterologous host for the expression of four structural clavulanic acid biosynthesis genes, which encode carboxyethylarginine synthase (ceas2), beta-lactam synthetase (bls2), clavaminate synthase (cas2), and proclavaminate amidinohydrolase (pah2). The genes are cloned into pIBR25 expression vector containing ermE promoter to generate pBS4. The cas2 gene is also cloned into pSET152 to generate pCas2. It is then integrated into the genome of Streptomyces venezuelae YJ028 | Streptomyces clavuligerus |
| 2.5.1.66 | Streptomyces venezuelae YJ028 is utilized as the heterologous host for the expression of four structural clavulanic acid biosynthesis genes, which encode carboxyethylarginine synthase (ceas2), beta-lactam synthetase (bls2), clavaminate synthase (cas2), and proclavaminate amidinohydrolase (pah2). The genes are cloned into pIBR25 expression vector containing ermE promoter to generate pBS4. The cas2 gene is also cloned into pSET152 to generate pCas2. It is then integrated into the genome of Streptomyces venezuelae YJ028 | Streptomyces clavuligerus |
| 3.5.3.22 | expression in Streptomyces venezuelae | Streptomyces clavuligerus |
| 3.5.3.22 | Streptomyces venezuelae YJ028 is utilized as the heterologous host for the expression of four structural clavulanic acid biosynthesis genes, which encode carboxyethylarginine synthase (ceas2), beta-lactam synthetase (bls2), clavaminate synthase (cas2), and proclavaminate amidinohydrolase (pah2). The genes are cloned into pIBR25 expression vector containing ermE promoter to generate pBS4. The cas2 gene is also cloned into pSET152 to generate pCas2. It is then integrated into the genome of Streptomyces venezuelae YJ028 | Streptomyces clavuligerus |
| 6.3.3.4 | Streptomyces venezuelae YJ028 is utilized as the heterologous host for the expression of four structural clavulanic acid biosynthesis genes, which encode carboxyethylarginine synthase (ceas2), beta-lactam synthetase (bls2), clavaminate synthase (cas2), and proclavaminate amidinohydrolase (pah2). The genes are cloned into pIBR25 expression vector containing ermE promoter to generate pBS4. The cas2 gene is also cloned into pSET152 to generate pCas2. It is then integrated into the genome of Streptomyces venezuelae YJ028 | Streptomyces clavuligerus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.21 | (3S,5S)-dihydroclavaminate + 2-oxoglutarate + O2 | Streptomyces clavuligerus | the enzyme is involved in clavulanic acid biosynthesis | (3S,5S)-clavaminate + succinate + CO2 + H2O | - |
? |  |
| 1.14.11.21 | proclavaminate + 2-oxoglutarate + O2 | Streptomyces clavuligerus | the enzyme is involved in clavulanic acid biosynthesis | dihydroclavaminate + succinate + CO2 + H2O | - |
? | |
| 2.5.1.66 | D-glyceraldehyde 3-phosphate + L-arginine | Streptomyces clavuligerus | the enzyme is involved in clavulanic acid biosynthesis | N2-(2-carboxyethyl)-L-arginine + phosphate | - |
? | |
| 3.5.3.22 | guanidinoproclavaminic acid + H2O | Streptomyces clavuligerus | the enzyme is involved in clavulanic acid biosynthesis | proclavaminic acid + urea | - |
? | |
| 6.3.3.4 | ATP + L-N2-(2-carboxyethyl)arginine | Streptomyces clavuligerus | the enzyme is involved in clavulanic acid biosynthesis | AMP + diphosphate + deoxyamidinoproclavaminate | - |
? | |
| 6.3.3.4 | ATP + L-N2-(2-carboxyethyl)arginine | Streptomyces clavuligerus DSM 738 | the enzyme is involved in clavulanic acid biosynthesis | AMP + diphosphate + deoxyamidinoproclavaminate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.11.21 | Streptomyces clavuligerus | Q53939 | - |
- |
| 2.5.1.66 | Streptomyces clavuligerus | - |
- |
- |
| 2.5.1.66 | Streptomyces clavuligerus | E2Q5Q8 | isoform ceaS2 | - |
| 3.5.3.22 | Streptomyces clavuligerus | B5GLC8 | - |
- |
| 3.5.3.22 | Streptomyces clavuligerus | P0DJQ3 | - |
- |
| 6.3.3.4 | Streptomyces clavuligerus | B5GLC7 | - |
- |
| 6.3.3.4 | Streptomyces clavuligerus DSM 738 | B5GLC7 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.21 | (3S,5S)-dihydroclavaminate + 2-oxoglutarate + O2 | the enzyme is involved in clavulanic acid biosynthesis | Streptomyces clavuligerus | (3S,5S)-clavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | proclavaminate + 2-oxoglutarate + O2 | the enzyme is involved in clavulanic acid biosynthesis | Streptomyces clavuligerus | dihydroclavaminate + succinate + CO2 + H2O | - |
? | |
| 2.5.1.66 | D-glyceraldehyde 3-phosphate + L-arginine | the enzyme is involved in clavulanic acid biosynthesis | Streptomyces clavuligerus | N2-(2-carboxyethyl)-L-arginine + phosphate | - |
? | |
| 3.5.3.22 | guanidinoproclavaminic acid + H2O | the enzyme is involved in clavulanic acid biosynthesis | Streptomyces clavuligerus | proclavaminic acid + urea | - |
? | |
| 6.3.3.4 | ATP + L-N2-(2-carboxyethyl)arginine | the enzyme is involved in clavulanic acid biosynthesis | Streptomyces clavuligerus | AMP + diphosphate + deoxyamidinoproclavaminate | - |
? | |
| 6.3.3.4 | ATP + L-N2-(2-carboxyethyl)arginine | the enzyme is involved in clavulanic acid biosynthesis | Streptomyces clavuligerus DSM 738 | AMP + diphosphate + deoxyamidinoproclavaminate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.11.21 | CAS2 | - |
Streptomyces clavuligerus |
| 2.5.1.66 | ceaS2 | - |
Streptomyces clavuligerus |
| 3.5.3.22 | PAH2 | - |
Streptomyces clavuligerus |
| 6.3.3.4 | bls2 | - |
Streptomyces clavuligerus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.11.21 | metabolism | the enzyme is involved in clavulanic acid biosynthesis | Streptomyces clavuligerus |
| 2.5.1.66 | metabolism | the enzyme is involved in clavulanic acid biosynthesis | Streptomyces clavuligerus |
| 3.5.3.22 | metabolism | the enzyme is involved in clavulanic acid biosynthesis | Streptomyces clavuligerus |
| 6.3.3.4 | metabolism | the enzyme is involved in clavulanic acid biosynthesis | Streptomyces clavuligerus |
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Release 2023.1 (January 2023)
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