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Literature summary extracted from
- Engineering a fungal peroxidase that degrades lignin at very acidic pH (2014), Biotechnol. Biofuels, 7, 114 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.11.1.13 | expression in Escherichia coli | Gelatoporia subvermispora |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.11.1.13 | S168W | mutation introduced to confer Vversatile peroxidase-type activity, EC 1.11.1.16, on aromatic substrates and dyes. Variant conserves the high catalytic efficiency of isoform MnP6 oxidizing Mn2+ and gains the ability to oxidize veratryl alcohol as well as reactive black 5 | Gelatoporia subvermispora |
| 1.11.1.13 | T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G | mutation introduced to confer Vversatile peroxidase-type activity, EC 1.11.1.16, on aromatic substrates and dyes. Variant conserves the high catalytic efficiency of isoform MnP6 oxidizing Mn2+ and gains the ability to oxidize veratryl alcohol as well as reactive black 5 | Gelatoporia subvermispora |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.13 | 0.0054 | - |
Reactive Black 5 | mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 | Gelatoporia subvermispora |  |
| 1.11.1.13 | 0.0085 | - |
Mn(II) | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
| 1.11.1.13 | 0.0087 | - |
Mn(II) | wild-type, 25°C, pH 5.0 | Gelatoporia subvermispora | |
| 1.11.1.13 | 0.011 | - |
Mn(II) | mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 | Gelatoporia subvermispora | |
| 1.11.1.13 | 0.0126 | - |
Reactive Black 5 | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
| 1.11.1.13 | 0.06 | - |
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
| 1.11.1.13 | 2.74 | - |
veratryl alcohol | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
| 1.11.1.13 | 21.6 | - |
veratryl alcohol | mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 | Gelatoporia subvermispora | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.11.1.13 | Gelatoporia subvermispora | M2REM6 | - |
- |
| 1.11.1.13 | Gelatoporia subvermispora B | M2REM6 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.13 | 2 Mn(II) + 2 H+ + H2O2 | - |
Gelatoporia subvermispora | 2 Mn(III) + 2 H2O | - |
? | |
| 1.11.1.13 | 2 Mn(II) + 2 H+ + H2O2 | - |
Gelatoporia subvermispora B | 2 Mn(III) + 2 H2O | - |
? | |
| 1.11.1.13 | 2 veratryl alcohol + H2O2 | no substrate of wild-type | Gelatoporia subvermispora | 2 veratraldehyde + H2O | - |
? | |
| 1.11.1.13 | 2 veratryl alcohol + H2O2 | no substrate of wild-type | Gelatoporia subvermispora B | 2 veratraldehyde + H2O | - |
? | |
| 1.11.1.13 | 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 | no substrate of wild-type | Gelatoporia subvermispora | ? | - |
? | |
| 1.11.1.13 | 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 | no substrate of wild-type | Gelatoporia subvermispora B | ? | - |
? | |
| 1.11.1.13 | Reactive Black 5 + H2O2 | no substrate of wild-type | Gelatoporia subvermispora | ? + H2O | - |
? | |
| 1.11.1.13 | Reactive Black 5 + H2O2 | no substrate of wild-type | Gelatoporia subvermispora B | ? + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.11.1.13 | MnP6 | - |
Gelatoporia subvermispora |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.13 | 0.54 | - |
veratryl alcohol | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
| 1.11.1.13 | 1.4 | - |
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
| 1.11.1.13 | 1.69 | - |
veratryl alcohol | mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 | Gelatoporia subvermispora | |
| 1.11.1.13 | 7 | - |
Reactive Black 5 | mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 | Gelatoporia subvermispora | |
| 1.11.1.13 | 7.8 | - |
Reactive Black 5 | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
| 1.11.1.13 | 60.5 | - |
Mn(II) | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
| 1.11.1.13 | 66.9 | - |
Mn(II) | mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 | Gelatoporia subvermispora | |
| 1.11.1.13 | 83 | - |
Mn(II) | wild-type, 25°C, pH 5.0 | Gelatoporia subvermispora | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.13 | 0.078 | - |
veratryl alcohol | mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 | Gelatoporia subvermispora | |
| 1.11.1.13 | 0.197 | - |
veratryl alcohol | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
| 1.11.1.13 | 23 | - |
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
| 1.11.1.13 | 619 | - |
Reactive Black 5 | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
| 1.11.1.13 | 1310 | - |
Reactive Black 5 | mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 | Gelatoporia subvermispora | |
| 1.11.1.13 | 6060 | - |
Mn(II) | mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 | Gelatoporia subvermispora | |
| 1.11.1.13 | 7110 | - |
Mn(II) | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
| 1.11.1.13 | 9540 | - |
Mn(II) | wild-type, 25°C, pH 5.0 | Gelatoporia subvermispora | |
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