EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.B20 | gene bdh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Escherichia coli | Bacillus licheniformis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.B20 | acetoin + NADH + H+ | Bacillus licheniformis | racemic mixture of (3S/3R)-acetoin | meso-2,3-butanediol + NAD+ | - |
r | |
1.1.1.B20 | acetoin + NADH + H+ | Bacillus licheniformis 10-1-A | racemic mixture of (3S/3R)-acetoin | meso-2,3-butanediol + NAD+ | - |
r | |
1.1.1.B20 | additional information | Bacillus licheniformis | stereoisomeric composition analysis of 2,3-butanediol produced by strain 10-1-A using gas chromatography. Strain 10-1-A produces a mixture of (2R,3R)-2,3-butanediol and meso-2,3-butanediol with a ratio of nearly 1:1. As (3R)-acetoin is the major source of (2R,3R)-2,3-butanediol and meso-2,3-butanediol, a meso-butanediol dehydrogenase and a (2R,3R)-2,3-butanediol dehydrogenase are co-present in strain 10-1-A | ? | - |
? | |
1.1.1.B20 | additional information | Bacillus licheniformis 10-1-A | stereoisomeric composition analysis of 2,3-butanediol produced by strain 10-1-A using gas chromatography. Strain 10-1-A produces a mixture of (2R,3R)-2,3-butanediol and meso-2,3-butanediol with a ratio of nearly 1:1. As (3R)-acetoin is the major source of (2R,3R)-2,3-butanediol and meso-2,3-butanediol, a meso-butanediol dehydrogenase and a (2R,3R)-2,3-butanediol dehydrogenase are co-present in strain 10-1-A | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.B20 | Bacillus licheniformis | X5F726 | isolated from soil samples | - |
1.1.1.B20 | Bacillus licheniformis 10-1-A | X5F726 | isolated from soil samples | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.B20 | additional information | optimization of culture conditions or strain 10-1-A and production of enzyme meso-2,3-BDH, overview | Bacillus licheniformis | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.B20 | (3R)-acetoin + NADH + H+ | - |
Bacillus licheniformis | meso-2,3-butanediol + NAD+ | - |
r | |
1.1.1.B20 | (3R)-acetoin + NADH + H+ | - |
Bacillus licheniformis 10-1-A | meso-2,3-butanediol + NAD+ | - |
r | |
1.1.1.B20 | acetoin + NADH + H+ | racemic mixture of (3S/3R)-acetoin | Bacillus licheniformis | meso-2,3-butanediol + NAD+ | - |
r | |
1.1.1.B20 | acetoin + NADH + H+ | racemic mixture of (3S/3R)-acetoin | Bacillus licheniformis 10-1-A | meso-2,3-butanediol + NAD+ | - |
r | |
1.1.1.B20 | additional information | stereoisomeric composition analysis of 2,3-butanediol produced by strain 10-1-A using gas chromatography. Strain 10-1-A produces a mixture of (2R,3R)-2,3-butanediol and meso-2,3-butanediol with a ratio of nearly 1:1. As (3R)-acetoin is the major source of (2R,3R)-2,3-butanediol and meso-2,3-butanediol, a meso-butanediol dehydrogenase and a (2R,3R)-2,3-butanediol dehydrogenase are co-present in strain 10-1-A | Bacillus licheniformis | ? | - |
? | |
1.1.1.B20 | additional information | stereoisomeric composition analysis of 2,3-butanediol produced by strain 10-1-A using gas chromatography. Strain 10-1-A produces a mixture of (2R,3R)-2,3-butanediol and meso-2,3-butanediol with a ratio of nearly 1:1. As (3R)-acetoin is the major source of (2R,3R)-2,3-butanediol and meso-2,3-butanediol, a meso-butanediol dehydrogenase and a (2R,3R)-2,3-butanediol dehydrogenase are co-present in strain 10-1-A | Bacillus licheniformis 10-1-A | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.B20 | BDH | - |
Bacillus licheniformis |
1.1.1.B20 | meso-BDH | - |
Bacillus licheniformis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.B20 | 50 | - |
- |
Bacillus licheniformis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.B20 | 7 | - |
- |
Bacillus licheniformis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.B20 | NAD+ | - |
Bacillus licheniformis | |
1.1.1.B20 | NADH | - |
Bacillus licheniformis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.B20 | physiological function | 2,3-butanediol dehydrogenase (BDH) catalyzes the interconversion between acetoin and 2,3-butanediol and is a key enzyme for 2,3-butanediol production | Bacillus licheniformis |