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Literature summary extracted from

  • Li, L.; Zhang, L.; Li, K.; Wang, Y.; Gao, C.; Han, B.; Ma, C.; Xu, P.
    A newly isolated Bacillus licheniformis strain thermophilically produces 2,3-butanediol, a platform and fuel bio-chemical (2013), Biotechnol. Biofuels, 6, 123 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.B20 gene bdh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Escherichia coli Bacillus licheniformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.B20 acetoin + NADH + H+ Bacillus licheniformis racemic mixture of (3S/3R)-acetoin meso-2,3-butanediol + NAD+
-
r
1.1.1.B20 acetoin + NADH + H+ Bacillus licheniformis 10-1-A racemic mixture of (3S/3R)-acetoin meso-2,3-butanediol + NAD+
-
r
1.1.1.B20 additional information Bacillus licheniformis stereoisomeric composition analysis of 2,3-butanediol produced by strain 10-1-A using gas chromatography. Strain 10-1-A produces a mixture of (2R,3R)-2,3-butanediol and meso-2,3-butanediol with a ratio of nearly 1:1. As (3R)-acetoin is the major source of (2R,3R)-2,3-butanediol and meso-2,3-butanediol, a meso-butanediol dehydrogenase and a (2R,3R)-2,3-butanediol dehydrogenase are co-present in strain 10-1-A ?
-
?
1.1.1.B20 additional information Bacillus licheniformis 10-1-A stereoisomeric composition analysis of 2,3-butanediol produced by strain 10-1-A using gas chromatography. Strain 10-1-A produces a mixture of (2R,3R)-2,3-butanediol and meso-2,3-butanediol with a ratio of nearly 1:1. As (3R)-acetoin is the major source of (2R,3R)-2,3-butanediol and meso-2,3-butanediol, a meso-butanediol dehydrogenase and a (2R,3R)-2,3-butanediol dehydrogenase are co-present in strain 10-1-A ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B20 Bacillus licheniformis X5F726 isolated from soil samples
-
1.1.1.B20 Bacillus licheniformis 10-1-A X5F726 isolated from soil samples
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.B20 additional information optimization of culture conditions or strain 10-1-A and production of enzyme meso-2,3-BDH, overview Bacillus licheniformis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B20 (3R)-acetoin + NADH + H+
-
Bacillus licheniformis meso-2,3-butanediol + NAD+
-
r
1.1.1.B20 (3R)-acetoin + NADH + H+
-
Bacillus licheniformis 10-1-A meso-2,3-butanediol + NAD+
-
r
1.1.1.B20 acetoin + NADH + H+ racemic mixture of (3S/3R)-acetoin Bacillus licheniformis meso-2,3-butanediol + NAD+
-
r
1.1.1.B20 acetoin + NADH + H+ racemic mixture of (3S/3R)-acetoin Bacillus licheniformis 10-1-A meso-2,3-butanediol + NAD+
-
r
1.1.1.B20 additional information stereoisomeric composition analysis of 2,3-butanediol produced by strain 10-1-A using gas chromatography. Strain 10-1-A produces a mixture of (2R,3R)-2,3-butanediol and meso-2,3-butanediol with a ratio of nearly 1:1. As (3R)-acetoin is the major source of (2R,3R)-2,3-butanediol and meso-2,3-butanediol, a meso-butanediol dehydrogenase and a (2R,3R)-2,3-butanediol dehydrogenase are co-present in strain 10-1-A Bacillus licheniformis ?
-
?
1.1.1.B20 additional information stereoisomeric composition analysis of 2,3-butanediol produced by strain 10-1-A using gas chromatography. Strain 10-1-A produces a mixture of (2R,3R)-2,3-butanediol and meso-2,3-butanediol with a ratio of nearly 1:1. As (3R)-acetoin is the major source of (2R,3R)-2,3-butanediol and meso-2,3-butanediol, a meso-butanediol dehydrogenase and a (2R,3R)-2,3-butanediol dehydrogenase are co-present in strain 10-1-A Bacillus licheniformis 10-1-A ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B20 BDH
-
Bacillus licheniformis
1.1.1.B20 meso-BDH
-
Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.B20 50
-
-
Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.B20 7
-
-
Bacillus licheniformis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B20 NAD+
-
Bacillus licheniformis
1.1.1.B20 NADH
-
Bacillus licheniformis

General Information

EC Number General Information Comment Organism
1.1.1.B20 physiological function 2,3-butanediol dehydrogenase (BDH) catalyzes the interconversion between acetoin and 2,3-butanediol and is a key enzyme for 2,3-butanediol production Bacillus licheniformis