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Literature summary extracted from

  • Kim, S.J.; Joo, J.C.; Song, B.K.; Yoo, Y.J.; Kim, Y.H.
    Engineering a horseradish peroxidase C stable to radical attacks by mutating multiple radical coupling sites (2015), Biotechnol. Bioeng., 112, 668-676 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.7 F130A/F142A/ F143A/F179A isoform HRP C, mutant shows marginal improvement in stability Armoracia rusticana
1.11.1.7 F142A isoform HRP C, mutation in a residue vulnerable to modification by phenoxyl radicals, mutant still exhibits rapid radical inactivation Armoracia rusticana
1.11.1.7 F143A isoform HRP C, mutation in a residue vulnerable to modification by phenoxyl radicals, mutant still exhibits rapid radical inactivation Armoracia rusticana
1.11.1.7 F179A isoform HRP C, mutation in a residue vulnerable to modification by phenoxyl radicals, mutant still exhibits rapid radical inactivation Armoracia rusticana
1.11.1.7 F68A isoform HRP C, mutation in a residue vulnerable to modification by phenoxyl radicals, mutant still exhibits rapid radical inactivation Armoracia rusticana
1.11.1.7 F68A/F142A/F143A/F179A isoform HRP C, dramatic enhancement of radical stability by retaining 41% of its initial activity in conditions when wild-type is completely inactivated Armoracia rusticana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.7 phenoxy radical phenylalanine residues are vulnerable to modification by phenoxyl radicals. Radical coupling does not change the secondary structure or the active site of HRP isoform C Armoracia rusticana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.7 0.966
-
 guaiacol mutant F130A, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 0.966
-
 guaiacol wild-type isoform C, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 1.06
-
 guaiacol mutant F68A, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 1.72
-
 guaiacol mutant F143A, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 1.82
-
 guaiacol mutant F179A, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 2.04
-
 guaiacol mutant F142A, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 2.16
-
 guaiacol mutant F130A/F142A/ F143A/F179A, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 2.69
-
 guaiacol mutant F68A/F142A/ F143A/F179A, pH 5, temperature not specified in the publication Armoracia rusticana

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Armoracia rusticana P80679
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.7 commercial preparation
-
 Armoracia rusticana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 2 guaiacol + H2O2
-
 Armoracia rusticana 3,3'-dimethoxy-4,4'-biphenylquinone + H2O
-
 ?
1.11.1.7 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) + H2O2
-
 Armoracia rusticana ? + H2O
-
 ?
1.11.1.7 phenol + H2O2
-
 Armoracia rusticana ? + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.7 HRP A2
-
 Armoracia rusticana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.7 0.952
-
 guaiacol mutant F68A, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 1.24
-
 guaiacol mutant F142A, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 1.41
-
 guaiacol mutant F130A, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 1.44
-
 guaiacol wild-type isoform C, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 1.55
-
 guaiacol mutant F143A, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 1.83
-
 guaiacol mutant F179A, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 2.09
-
 guaiacol mutant F130A/F142A/ F143A/F179A, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 2.3
-
 guaiacol mutant F68A/F142A/ F143A/F179A, pH 5, temperature not specified in the publication Armoracia rusticana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.7 0.61
-
 guaiacol mutant F142A, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 0.86
-
 guaiacol mutant F68A/F142A/ F143A/F179A, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 0.89
-
 guaiacol mutant F143A, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 0.89
-
 guaiacol mutant F68A, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 1.01
-
 guaiacol mutant F179A, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 1.07
-
 guaiacol mutant F130A/F142A/ F143A/F179A, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 1.46
-
 guaiacol mutant F130A, pH 5, temperature not specified in the publication Armoracia rusticana
1.11.1.7 1.49
-
 guaiacol wild-type isoform C, pH 5, temperature not specified in the publication Armoracia rusticana