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Literature summary extracted from

  • Srikrishnan, S.; Randall, A.; Baldi, P.; Da Silva, N.A.
    Rationally selected single-site mutants of the Thermoascus aurantiacus endoglucanase increase hydrolytic activity on cellulosic substrates (2012), Biotechnol. Bioeng., 109, 1595-1599 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.203 expression in Pichia pastoris Thermoascus aurantiacus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.203 F16S 1.7fold increase in kcat and 1.5fold improvement in hydrolytic activity on cellulosic substrates, while maintaining thermostability Thermoascus aurantiacus
3.2.1.203 F16S/Y95F 60% reduction in catalytic efficiency Thermoascus aurantiacus
3.2.1.203 Y95F 4.0fold increase in kcat and 2.5fold improvement in hydrolytic activity on cellulosic substrates, while maintaining thermostability Thermoascus aurantiacus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.203 additional information
-
 carboxymethyl cellulose Km value is 0.14 mg/ml, 0.17 mg/ml, 0.13 mg/ml, and 0.17 mg/ml for wild-type, mutant F16S, mutant Y95F and mutant F16S/Y95F, respectively Thermoascus aurantiacus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.203 Thermoascus aurantiacus Q96UI5
-
-
3.2.1.203 Thermoascus aurantiacus IFO 9748 Q96UI5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.203 carboxymethyl cellulose + H2O
-
 Thermoascus aurantiacus carboxymethyl cellulose with endohydrolysed (1->4)-beta-D-glucosidic linkages
-
 ?
3.2.1.203 carboxymethyl cellulose + H2O
-
 Thermoascus aurantiacus IFO 9748 carboxymethyl cellulose with endohydrolysed (1->4)-beta-D-glucosidic linkages
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.203 EG1
-
 Thermoascus aurantiacus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.203 70
-
 wild-type and mutants F16S and Y95F Thermoascus aurantiacus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.203 50
-
 wild-type and mutants F16S and Y95F, active for more than 12 h Thermoascus aurantiacus
3.2.1.203 70
-
 wild-type and mutants F16S and Y95F, 2 h, more than 80% residual activity Thermoascus aurantiacus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.203 1.95
-
 carboxymethyl cellulose mutant F16S/Y95F, pH 5.0, 70°C Thermoascus aurantiacus
3.2.1.203 3.98
-
 carboxymethyl cellulose wild-type, pH 5.0, 70°C Thermoascus aurantiacus
3.2.1.203 7.98
-
 carboxymethyl cellulose mutant F16S, pH 5.0, 70°C Thermoascus aurantiacus
3.2.1.203 16.58
-
 carboxymethyl cellulose mutant Y95F, pH 5.0, 70°C Thermoascus aurantiacus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.203 5
-
 wild-type and mutants F16S and Y95F Thermoascus aurantiacus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.203 4 8 wild-type and mutants F16S and Y95F Thermoascus aurantiacus