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Literature summary extracted from
- Purification and biochemical characteristics of beta-D-glucosidase from a thermophilic fungus, Thermomyces lanuginosus-SSBP (1999), Biotechnol. Appl. Biochem., 30, 81-87 .
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | cysteine | - |
Thermomyces lanuginosus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | cellobiose | competitive | Thermomyces lanuginosus | |
| 3.2.1.21 | D-glucose | competitive | Thermomyces lanuginosus | |
| 3.2.1.21 | Hg2+ | 2 mM, complete inhibition | Thermomyces lanuginosus | |
| 3.2.1.21 | N-bromosuccinimide | 2 mM, complete inhibition | Thermomyces lanuginosus | |
| 3.2.1.21 | Salicin | competitive | Thermomyces lanuginosus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.075 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.5, 50°C | Thermomyces lanuginosus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.21 | extracellular | - |
Thermomyces lanuginosus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 200000 | - |
gel filtration | Thermomyces lanuginosus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Thermomyces lanuginosus | - |
- |
- |
| 3.2.1.21 | Thermomyces lanuginosus SSBP | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 7.6 | - |
substrate cellobiose, pH 6.5, 50°C | Thermomyces lanuginosus |
| 3.2.1.21 | 9.65 | - |
substrate 4-nitrophenyl beta-D-glucopyranoside, pH 6.5, 50°C | Thermomyces lanuginosus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermomyces lanuginosus | 4-nitrophenol + D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermomyces lanuginosus SSBP | 4-nitrophenol + D-glucose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | - |
Thermomyces lanuginosus | 2 D-glucose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | - |
Thermomyces lanuginosus SSBP | 2 D-glucose | - |
? | |
| 3.2.1.21 | additional information | enzyme requires glucoside substrates containing a terminal, non-reducing beta-D-glucose residue. Enzyme exhibited little, but significant alpha-D-glucosidase activity and is capable of hydrolysing beta-1,6-linked diglucosides and gentiobiose | Thermomyces lanuginosus | ? | - |
? | |
| 3.2.1.21 | additional information | enzyme requires glucoside substrates containing a terminal, non-reducing beta-D-glucose residue. Enzyme exhibited little, but significant alpha-D-glucosidase activity and is capable of hydrolysing beta-1,6-linked diglucosides and gentiobiose | Thermomyces lanuginosus SSBP | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | dimer | 2 * 105000, SDS-PAGE | Thermomyces lanuginosus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 65 | - |
- |
Thermomyces lanuginosus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 50 | - |
30 min, stable | Thermomyces lanuginosus |
| 3.2.1.21 | 70 | - |
30 min, complete loss of activity | Thermomyces lanuginosus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 44.4 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.5, 50°C | Thermomyces lanuginosus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 6 | - |
- |
Thermomyces lanuginosus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5 | - |
stable when the pH of the storage buffer is above 5.0 | Thermomyces lanuginosus |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.52 | - |
cellobiose | pH 6.5, 50°C | Thermomyces lanuginosus | |
| 3.2.1.21 | 0.55 | - |
D-glucose | pH 6.5, 50°C | Thermomyces lanuginosus | |
| 3.2.1.21 | 0.81 | - |
Salicin | pH 6.5, 50°C | Thermomyces lanuginosus | |
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