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Literature summary extracted from
- cDNA cloning and characterization of vanadium-dependent bromoperoxidases from the red alga Laurencia nipponica (2014), Biosci. Biotechnol. Biochem., 78, 1310-1319 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.11.1.18 | expressed in Escherichia coli BL21(DE3) cells | Laurencia nipponica |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.18 | 0.025 | - |
H2O2 | isoform VBPO2, at pH 6.0 and 25°C | Laurencia nipponica |  |
| 1.11.1.18 | 0.026 | - |
H2O2 | isoform VBPO1, at pH 6.0 and 25°C | Laurencia nipponica | |
| 1.11.1.18 | 0.35 | - |
Br- | isoform VBPO2, at pH 6.0 and 25°C | Laurencia nipponica | |
| 1.11.1.18 | 0.53 | - |
Br- | isoform VBPO1, at pH 6.0 and 25°C | Laurencia nipponica | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.18 | Vanadium | dependent on | Laurencia nipponica | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.11.1.18 | Laurencia nipponica | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.11.1.18 | ammonium sulfate precipitation, Ni-NTA column chromatography, and DE52 gel filtration | Laurencia nipponica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.18 | Br- + H2O2 + (3E,6R,7R)-laurediol | - |
Laurencia nipponica | deacetyllaurencin + H2O | - |
? | |
| 1.11.1.18 | Br- + H2O2 + monochlorodimedone | - |
Laurencia nipponica | monobromo-monochlorodimedone + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.11.1.18 | ? | x * 77000, SDS-PAGE | Laurencia nipponica |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.11.1.18 | vanadium-dependent bromoperoxidase | - |
Laurencia nipponica |
| 1.11.1.18 | VBPO1 | isoform | Laurencia nipponica |
| 1.11.1.18 | VBPO2 | isoform | Laurencia nipponica |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.18 | 7 | - |
- |
Laurencia nipponica |
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Release 2023.1 (January 2023)
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