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Literature summary extracted from

  • Takashima, S.; Nakamura, A.; Masaki, H.; Uozumi, T.
    Purification and characterization of cellulases from Humicola grisea (1996), Biosci. Biotechnol. Biochem., 60, 77-82 .
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.12
-
 4-nitrophenyl alpha-D-glucopyranoside isoform beta-glucosidase 6, pH 5.0, 50°C [Humicola] grisea var. thermoidea
3.2.1.21 0.16
-
 4-nitrophenyl alpha-D-glucopyranoside isoform beta-glucosidase 1, pH 5.0, 50°C [Humicola] grisea var. thermoidea
3.2.1.21 0.22
-
 4-nitrophenyl alpha-D-glucopyranoside isoform beta-glucosidase 3, pH 5.0, 50°C [Humicola] grisea var. thermoidea
3.2.1.21 0.25
-
 4-nitrophenyl alpha-D-glucopyranoside isoform beta-glucosidase 2, pH 5.0, 50°C [Humicola] grisea var. thermoidea
3.2.1.21 0.34
-
 4-nitrophenyl alpha-D-glucopyranoside isoform beta-glucosidase 4, pH 5.0, 50°C [Humicola] grisea var. thermoidea
3.2.1.21 0.56
-
 4-nitrophenyl alpha-D-glucopyranoside isoform beta-glucosidase 5, pH 5.0, 50°C [Humicola] grisea var. thermoidea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 80000
-
 gel filtration, isoform beta-glucosidase 5 [Humicola] grisea var. thermoidea
3.2.1.21 300000
-
 gel filtration, isoform beta-glucosidase 1 [Humicola] grisea var. thermoidea
3.2.1.21 460000
-
 gel filtration, isoform beta-glucosidase 2 [Humicola] grisea var. thermoidea
3.2.1.21 460000
-
 gel filtration, isoform beta-glucosidase 3 [Humicola] grisea var. thermoidea

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 [Humicola] grisea var. thermoidea
-
-
-
3.2.1.4 [Humicola] grisea var. thermoidea IFO 9854
-
-
-
3.2.1.21 [Humicola] grisea var. thermoidea
-
-
-
3.2.1.21 [Humicola] grisea var. thermoidea IFO 9854
-
-
-
3.2.1.74 [Humicola] grisea var. thermoidea
-
-
-
3.2.1.74 [Humicola] grisea var. thermoidea IFO 9854
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 [Humicola] grisea var. thermoidea
3.2.1.21
-
 [Humicola] grisea var. thermoidea
3.2.1.74
-
 [Humicola] grisea var. thermoidea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 9.06
-
 isoform endoglucanase 1, pH 5.0, 50°C [Humicola] grisea var. thermoidea
3.2.1.4 24.1
-
 isoform endoglucanase 2, pH 5.0, 50°C [Humicola] grisea var. thermoidea
3.2.1.21 7.1
-
 isoform beta-glucosidase 4, pH 5.0, 50°C [Humicola] grisea var. thermoidea
3.2.1.21 11.9
-
 isoform beta-glucosidase 3, pH 5.0, 50°C [Humicola] grisea var. thermoidea
3.2.1.21 12
-
 isoform beta-glucosidase 2, pH 5.0, 50°C [Humicola] grisea var. thermoidea
3.2.1.21 17.5
-
 isoform beta-glucosidase 5, pH 5.0, 50°C [Humicola] grisea var. thermoidea
3.2.1.21 31.8
-
 isoform beta-glucosidase 6, pH 5.0, 50°C [Humicola] grisea var. thermoidea
3.2.1.21 76.7
-
 isoform beta-glucosidase 1, pH 5.0, 50°C [Humicola] grisea var. thermoidea
3.2.1.74 1.14
-
 substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 5.0, 50°C [Humicola] grisea var. thermoidea
3.2.1.74 2.57
-
 substrate carboxymethyl cellulose, pH 5.0, 50°C [Humicola] grisea var. thermoidea
3.2.1.74 8.09
-
 substrate 4-nitrophenyl beta-D-cellobioside, pH 5.0, 50°C [Humicola] grisea var. thermoidea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O
-
 [Humicola] grisea var. thermoidea ?
-
 ?
3.2.1.21 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 [Humicola] grisea var. thermoidea 4-nitrophenol + D-glucose
-
 ?
3.2.1.21 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 [Humicola] grisea var. thermoidea IFO 9854 4-nitrophenol + D-glucose
-
 ?
3.2.1.21 additional information beta-glucosidases show poor activity on 4-nitrophenyl beta-D-cellobioside or cellooligosaccharides [Humicola] grisea var. thermoidea ?
-
 ?
3.2.1.21 additional information beta-glucosidases show poor activity on 4-nitrophenyl beta-D-cellobioside or cellooligosaccharides [Humicola] grisea var. thermoidea IFO 9854 ?
-
 ?
3.2.1.74 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 [Humicola] grisea var. thermoidea 4-nitrophenol + D-glucose
-
 ?
3.2.1.74 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 [Humicola] grisea var. thermoidea IFO 9854 4-nitrophenol + D-glucose
-
 ?
3.2.1.74 4-nitrophenyl beta-D-cellobioside + H2O
-
 [Humicola] grisea var. thermoidea 4-nitrophenol + beta-D-cellobiose
-
 ?
3.2.1.74 4-nitrophenyl beta-D-cellobioside + H2O
-
 [Humicola] grisea var. thermoidea IFO 9854 4-nitrophenol + beta-D-cellobiose
-
 ?
3.2.1.74 carboxymethyl cellulose + H2O
-
 [Humicola] grisea var. thermoidea ?
-
 ?
3.2.1.74 carboxymethyl cellulose + H2O
-
 [Humicola] grisea var. thermoidea IFO 9854 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 45000, isoform endoglucanase 1, x * 46500, isoform endoglucanse 2, SDS-PAGE [Humicola] grisea var. thermoidea
3.2.1.21 oligomer x * 115000, isoform beta-glucosidase 1, x * 94000 plus x * 21000, isoform beta-glucosidase 2, x * 94000 plus x * 21000, isoform beta-glucosidase 3, x * 52000, isoform beta-glucosidase 4, x * 49000 plus x * 46000, isoform beta-glucosidase 5, x * 77000, isoform beta-glucosidase 6, SDS-PAGE [Humicola] grisea var. thermoidea
3.2.1.74 ? x * 38500, SDS-PAGE [Humicola] grisea var. thermoidea