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Literature summary extracted from

  • Katrolia, P.; Jia, H.; Yan, Q.; Song, S.; Jiang, Z.; Xu, H.
    Characterization of a protease-resistant alpha-galactosidase from the thermophilic fungus Rhizomucor miehei and its application in removal of raffinose family oligosaccharides (2012), Biores. Technol., 110, 578-586 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.22 degradation enzyme completely hydrolyzes raffinose and stachyose present in soybeans and kidney beans at 50°C within 60 min Rhizomucor miehei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.22 expression in Escherichia coli Rhizomucor miehei

General Stability

EC Number General Stability Organism
3.2.1.22 enzyme displays remarkable resistance to proteases Rhizomucor miehei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 Ag+ 4% of initial activity Rhizomucor miehei
3.2.1.22 Hg2+ 8% of initial activity Rhizomucor miehei
3.2.1.22 additional information not inhibitory: D-galactose at 100 mM, or dithiothreitol, EDTA Rhizomucor miehei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.36
-
 4-nitrophenyl alpha-D-galactopyranoside pH 4.5, 50°C Rhizomucor miehei
3.2.1.22 16.9
-
 melibiose pH 4.5, 50°C Rhizomucor miehei
3.2.1.22 27.6
-
 stachyose pH 4.5, 50°C Rhizomucor miehei
3.2.1.22 47.9
-
 raffinose pH 4.5, 50°C Rhizomucor miehei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.22 Ca2+ 129% of initial activity Rhizomucor miehei
3.2.1.22 Co2+ 195% of initial activity Rhizomucor miehei
3.2.1.22 Cu2+ 119% of initial activity Rhizomucor miehei
3.2.1.22 Fe2+ 194% of initial activity Rhizomucor miehei
3.2.1.22 Zn2+ 116% of initial activity Rhizomucor miehei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 312000
-
 gel filtration Rhizomucor miehei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Rhizomucor miehei H8Y263
-
-
3.2.1.22 Rhizomucor miehei CAU432 H8Y263
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 198
-
 substrate 4-nitrophenyl alpha-D-galactopyranoside, pH 4.5, 50°C Rhizomucor miehei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Rhizomucor miehei 4-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 4-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Rhizomucor miehei CAU432 4-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 melibiose + H2O 5% of the activity with 4-nitrophenyl alpha-D-galactopyranoside Rhizomucor miehei alpha-D-galactose + alpha-D-glucose
-
 ?
3.2.1.22 melibiose + H2O 5% of the activity with 4-nitrophenyl alpha-D-galactopyranoside Rhizomucor miehei CAU432 alpha-D-galactose + alpha-D-glucose
-
 ?
3.2.1.22 raffinose + H2O 13.5% of the activity with 4-nitrophenyl alpha-D-galactopyranoside Rhizomucor miehei D-galactose + sucrose
-
 ?
3.2.1.22 raffinose + H2O 13.5% of the activity with 4-nitrophenyl alpha-D-galactopyranoside Rhizomucor miehei CAU432 D-galactose + sucrose
-
 ?
3.2.1.22 stachyose + H2O 31% of the activity with 4-nitrophenyl alpha-D-galactopyranoside Rhizomucor miehei raffinose + D-galactose
-
 ?
3.2.1.22 stachyose + H2O 31% of the activity with 4-nitrophenyl alpha-D-galactopyranoside Rhizomucor miehei CAU432 raffinose + D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 tetramer 4 * 85141, calculated, 4 * 85000, SDS-PAGE Rhizomucor miehei

Synonyms

EC Number Synonyms Comment Organism
3.2.1.22 Gal36
-
 Rhizomucor miehei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 60
-
-
 Rhizomucor miehei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 55
-
 30 min, 100% residual activity Rhizomucor miehei
3.2.1.22 60
-
 30 min, 70% reidual activity Rhizomucor miehei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.22 0.65
-
 melibiose pH 4.5, 50°C Rhizomucor miehei
3.2.1.22 4.2
-
 raffinose pH 4.5, 50°C Rhizomucor miehei
3.2.1.22 6.3
-
 4-nitrophenyl alpha-D-galactopyranoside pH 4.5, 50°C Rhizomucor miehei
3.2.1.22 6.4
-
 stachyose pH 4.5, 50°C Rhizomucor miehei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 4.5
-
-
 Rhizomucor miehei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.22 4.5 10
-
 Rhizomucor miehei

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.22 Rhizomucor miehei isoelectric focusing
-
 5.8

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.22 0.04
-
 melibiose pH 4.5, 50°C Rhizomucor miehei
3.2.1.22 0.09
-
 raffinose pH 4.5, 50°C Rhizomucor miehei
3.2.1.22 0.23
-
 stachyose pH 4.5, 50°C Rhizomucor miehei
3.2.1.22 17.5
-
 4-nitrophenyl alpha-D-galactopyranoside pH 4.5, 50°C Rhizomucor miehei