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Literature summary extracted from
- Substrate and inhibitor specificity of kynurenine monooxygenase from Cytophaga hutchinsonii (2017), Bioorg. Med. Chem. Lett., 27, 1705-1708 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.14.13.9 | medicine | the enzyme is a potential drug target for treatment of neurodegenerative disorders such as Huntington's and Alzheimer's diseases | Cytophaga hutchinsonii |
| 1.14.13.9 | pharmacology | the enzyme is a potential drug target for treatment of neurodegenerative disorders such as Huntington's and Alzheimer's diseases | Cytophaga hutchinsonii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.9 | 3,4-dichlorohippuric acid | - |
Cytophaga hutchinsonii |  |
| 1.14.13.9 | 3,4-dimethoxyhippuric acid | - |
Cytophaga hutchinsonii | |
| 1.14.13.9 | 3,5-dibromo-L-kynurenine | potent competitive inhibitor | Cytophaga hutchinsonii | |
| 1.14.13.9 | 3-chloro-DL-kynurenine | - |
Cytophaga hutchinsonii | |
| 1.14.13.9 | 3-methyl-DL-kynurenine | - |
Cytophaga hutchinsonii | |
| 1.14.13.9 | 5-bromo-3-chloro-DL-kynurenine | - |
Cytophaga hutchinsonii | |
| 1.14.13.9 | 5-bromo-3-methyl-DL-kynurenine | - |
Cytophaga hutchinsonii | |
| 1.14.13.9 | additional information | kynurenines substituted with a halogen at the 5-position are excellent substrates, with values of kcat and kcat/Km comparable to or higher than kynurenine. Kynurenines substituted in the 3-position are competitive inhibitors, with KI values lower than the Km for kynurenine. Bromination also enhances inhibition. A pharmacophore model of kynurenine monooxygenase is developed, and predicted that 3,4-dichlorohippuric acid would be an inhibitor | Cytophaga hutchinsonii |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.9 | 0.002 | - |
5-chloro-L-kynurenine | pH and temperature not specified in the publication | Cytophaga hutchinsonii | |
| 1.14.13.9 | 0.0045 | - |
5-bromo-L-kynurenine | pH and temperature not specified in the publication | Cytophaga hutchinsonii | |
| 1.14.13.9 | 0.067 | - |
L-kynurenine | pH and temperature not specified in the publication | Cytophaga hutchinsonii | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.9 | L-kynurenine + NADPH + H+ + O2 | Cytophaga hutchinsonii | - |
3-hydroxy-L-kynurenine + NADP+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.9 | Cytophaga hutchinsonii | Q11PP7 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.9 | 5-bromo-L-kynurenine + NADPH + H+ + O2 | - |
Cytophaga hutchinsonii | 5-bromo-3-hydroxy-L-kynurenine + NADP+ + H2O | - |
? | |
| 1.14.13.9 | 5-chloro-L-kynurenine + NADPH + H+ + O2 | - |
Cytophaga hutchinsonii | 5-chloro-3-hydroxy-L-kynurenine + NADP+ + H2O | - |
? | |
| 1.14.13.9 | L-kynurenine + NADPH + H+ + O2 | - |
Cytophaga hutchinsonii | 3-hydroxy-L-kynurenine + NADP+ + H2O | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.9 | 0.6 | - |
5-bromo-L-kynurenine | pH and temperature not specified in the publication | Cytophaga hutchinsonii | |
| 1.14.13.9 | 0.8 | - |
5-chloro-L-kynurenine | pH and temperature not specified in the publication | Cytophaga hutchinsonii | |
| 1.14.13.9 | 2 | - |
L-kynurenine | pH and temperature not specified in the publication | Cytophaga hutchinsonii | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.9 | 0.0015 | - |
3,5-dibromo-L-kynurenine | pH and temperature not specified in the publication | Cytophaga hutchinsonii | |
| 1.14.13.9 | 0.0086 | - |
3-methyl-DL-kynurenine | pH and temperature not specified in the publication | Cytophaga hutchinsonii | |
| 1.14.13.9 | 0.0102 | - |
5-bromo-3-methyl-DL-kynurenine | pH and temperature not specified in the publication | Cytophaga hutchinsonii | |
| 1.14.13.9 | 0.011 | - |
5-bromo-3-chloro-DL-kynurenine | pH and temperature not specified in the publication | Cytophaga hutchinsonii | |
| 1.14.13.9 | 0.0138 | - |
3-chloro-DL-kynurenine | pH and temperature not specified in the publication | Cytophaga hutchinsonii | |
| 1.14.13.9 | 0.034 | - |
3,4-dichlorohippuric acid | pH and temperature not specified in the publication | Cytophaga hutchinsonii | |
| 1.14.13.9 | 1 | - |
3,4-dimethoxyhippuric acid | pH and temperature not specified in the publication | Cytophaga hutchinsonii | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.13.9 | drug target | the enzyme is a potential drug target for treatment of neurodegenerative disorders such as Huntington's and Alzheimer's diseases | Cytophaga hutchinsonii |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.9 | 29.9 | - |
L-kynurenine | pH and temperature not specified in the publication | Cytophaga hutchinsonii | |
| 1.14.13.9 | 133.3 | - |
5-bromo-L-kynurenine | pH and temperature not specified in the publication | Cytophaga hutchinsonii | |
| 1.14.13.9 | 400 | - |
5-chloro-L-kynurenine | pH and temperature not specified in the publication | Cytophaga hutchinsonii | |
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