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Literature summary extracted from

  • Zhang, J.; Weng, H.; Ding, W.; Kang, Z.
    N-terminal engineering of glutamyl-tRNA reductase with positive charge arginine to increase 5-aminolevulinic acid biosynthesis (2017), Bioengineered, 8, 424-427 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.2.1.70 synthesis the recombinant engineered GluTR variant R1 can be used for improvement of the C5 pathway to enhance 5-aminolevulinic acid and other products Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.70 additional information N-terminal engineering of glutamyl-tRNA reductase with positive charge arginine to increase 5-aminolevulinic acid biosynthesis. Insertion of lysine or arginine residues (especially one arginine residue) behind Thr2 of GluTR significantly increased the stability of GluTR. By co-expression of this GluTR variant R1 and the glutamate-1-semialdehyde aminotransferase, 5-aminolevulinic acid production is improved 1.76fold to 1220 mg/L Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.70 heme feedback inhibition Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.70 L-glutamate 1-semialdehyde + NADP+ + tRNAGlu Escherichia coli
-
 L-glutamyl-tRNAGlu + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.70 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.70 L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
 Escherichia coli L-glutamyl-tRNAGlu + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.70 GluTR
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.70 NADP+
-
 Escherichia coli
1.2.1.70 NADPH
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
1.2.1.70 physiological function glutamyl-tRNA reductase (GluTR) is the first key enzyme of C5 pathway, it is feedback regulated by heme, and its N-terminus plays a critical role on its stability control Escherichia coli