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Literature summary extracted from

  • Trubitsina, L.I.; Tishchenko, S.V.; Gabdulkhakov, A.G.; Lisov, A.V.; Zakharova, M.V.; Leontievsky, A.A.
    Structural and functional characterization of two-domain laccase from Streptomyces viridochromogenes (2015), Biochimie, 112, 151-159 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.10.3.2 environmental protection laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. The enzyme can also be considered as a candidate for treating industrial effluent containing malachite green Streptomyces viridochromogenes

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 overproduced in Escherichia coli Streptomyces viridochromogenes
7.1.1.9 overproduced in Escherichia coli Streptomyces viridochromogenes

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.10.3.2 hanging-drop vapour-diffusion method, X-ray quality crystals are generated and the structure is solved to a resolution of 2.4 A Streptomyces viridochromogenes
7.1.1.9 hanging-drop vapour-diffusion method, X-ray quality crystals are generated and the structure is solved to a resolution of 2.4 A Streptomyces viridochromogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 EDTA
-
 Streptomyces viridochromogenes
1.10.3.2 additional information the enzyme is resistant to specific inhibitors of copper-containing oxidases, such as NaN3 and NaF Streptomyces viridochromogenes
7.1.1.9 EDTA
-
 Streptomyces viridochromogenes
7.1.1.9 additional information the enzyme is resistant to specific inhibitors of copper-containing oxidases, such as NaN3 and NaF Streptomyces viridochromogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.3
-
 guaiacol pH 8.5, temperature not specified in the publication Streptomyces viridochromogenes
1.10.3.2 0.38
-
 2,6-dimethoxyphenol pH 8.5, temperature not specified in the publication Streptomyces viridochromogenes
1.10.3.2 4.5
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.0, temperature not specified in the publication Streptomyces viridochromogenes
1.10.3.2 9.9
-
 K4[Fe(CN)6] pH 4.0, temperature not specified in the publication Streptomyces viridochromogenes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 cytoplasm the recombinant protein is found in the cytoplasmic fraction Streptomyces viridochromogenes 5737
-
7.1.1.9 cytoplasm the recombinant protein is found in the cytoplasmic fraction Streptomyces viridochromogenes 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ copper-containing enzyme. Contains 4 atoms per molecule of the enzyme Streptomyces viridochromogenes
7.1.1.9 Cu2+ copper-containing enzyme. Contains 4 atoms per molecule of the enzyme Streptomyces viridochromogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 88000 90000 gel filtration Streptomyces viridochromogenes
7.1.1.9 88000 90000 gel filtration Streptomyces viridochromogenes

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Streptomyces viridochromogenes J9S5G3
-
-
1.10.3.2 Streptomyces viridochromogenes VKM Ac-629 J9S5G3
-
-
7.1.1.9 Streptomyces viridochromogenes J9S5G3
-
-
7.1.1.9 Streptomyces viridochromogenes VKM Ac-629 J9S5G3
-
-

Storage Stability

EC Number Storage Stability Organism
1.10.3.2 20°C, 2 days, pH 6.0-10.0, the enzyme retains about 70% residual activity Streptomyces viridochromogenes
7.1.1.9 20°C, 2 days, pH 6.0-10.0, the enzyme retains about 70% residual activity Streptomyces viridochromogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Streptomyces viridochromogenes ?
-
 ?
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Streptomyces viridochromogenes VKM Ac-629 ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Streptomyces viridochromogenes ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Streptomyces viridochromogenes VKM Ac-629 ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Streptomyces viridochromogenes ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Streptomyces viridochromogenes VKM Ac-629 ?
-
 ?
1.10.3.2 K4[Fe(CN)6] + O2
-
 Streptomyces viridochromogenes ?
-
 ?
1.10.3.2 additional information phenolic compounds are oxidized in the presence of the enzyme under alkaline but not acidic conditions. Conversely, nonphenolic compounds are oxidized at acidic but not alkaline pH. The enzyme catalyses oxidation of nonphenolic compounds more efficiently than that of phenols. The two-domain laccase displays a cytochrome c oxidase activity and exhibits no ferroxidase activity Streptomyces viridochromogenes ?
-
 ?
1.10.3.2 additional information phenolic compounds are oxidized in the presence of the enzyme under alkaline but not acidic conditions. Conversely, nonphenolic compounds are oxidized at acidic but not alkaline pH. The enzyme catalyses oxidation of nonphenolic compounds more efficiently than that of phenols. The two-domain laccase displays a cytochrome c oxidase activity and exhibits no ferroxidase activity Streptomyces viridochromogenes VKM Ac-629 ?
-
 ?
7.1.1.9 4 ferrocytochrome c + O2 + 4 H+
-
 Streptomyces viridochromogenes 4 ferricytochrome c + 2 H2O
-
 ?
7.1.1.9 4 ferrocytochrome c + O2 + 4 H+
-
 Streptomyces viridochromogenes VKM Ac-629 4 ferricytochrome c + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 homotrimer 3 * 33900, calculated from sequence Streptomyces viridochromogenes
1.10.3.2 homotrimer 3 * 39000, SDS-PAGE after boiling Streptomyces viridochromogenes
7.1.1.9 homotrimer 3 * 33900, calculated from sequence Streptomyces viridochromogenes
7.1.1.9 homotrimer 3 * 39000, SDS-PAGE after boiling Streptomyces viridochromogenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 0.2
-
 K4[Fe(CN)6] pH 4.0, temperature not specified in the publication Streptomyces viridochromogenes
1.10.3.2 1.9
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.0, temperature not specified in the publication Streptomyces viridochromogenes
1.10.3.2 8
-
 guaiacol pH 8.5, temperature not specified in the publication Streptomyces viridochromogenes
1.10.3.2 34.4
-
 2,6-dimethoxyphenol pH 8.5, temperature not specified in the publication Streptomyces viridochromogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3
-
 below, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Streptomyces viridochromogenes
1.10.3.2 8.5
-
 substrate: 2,6-dimethoxyphenol Streptomyces viridochromogenes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 additional information
-
 phenolic compounds are oxidized in the presence of the enzyme under alkaline but not acidic conditions. Conversely, nonphenolic compounds are oxidized at acidic but not alkaline pH Streptomyces viridochromogenes

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 2
-
 20°C, 2 days, the enzyme retains about 30% residual activity Streptomyces viridochromogenes
1.10.3.2 3
-
 20°C, 2 days, the enzyme retains about 42% residual activity Streptomyces viridochromogenes
1.10.3.2 4
-
 20°C, 2 days, the enzyme retains about 65-60% residual activity Streptomyces viridochromogenes
1.10.3.2 5
-
 20°C, 2 days, the enzyme retains about 65-60% residual activity Streptomyces viridochromogenes
1.10.3.2 6 10 20°C, 2 days, the enzyme retains about 70% residual activity Streptomyces viridochromogenes
7.1.1.9 2
-
 20°C, 2 days, the enzyme retains about 30% residual activity Streptomyces viridochromogenes
7.1.1.9 3
-
 20°C, 2 days, the enzyme retains about 42% residual activity Streptomyces viridochromogenes
7.1.1.9 4
-
 20°C, 2 days, the enzyme retains about 65-60% residual activity Streptomyces viridochromogenes
7.1.1.9 5
-
 20°C, 2 days, the enzyme retains about 65-60% residual activity Streptomyces viridochromogenes
7.1.1.9 6 10 20°C, 2 days, the enzyme retains about 70% residual activity Streptomyces viridochromogenes

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.10.3.2 8
-
 pH 8.5, temperature not specified in the publication Streptomyces viridochromogenes EDTA
1.10.3.2 14
-
 pH 4.0, temperature not specified in the publication Streptomyces viridochromogenes EDTA

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 0.02
-
 K4[Fe(CN)6] pH 4.0, temperature not specified in the publication Streptomyces viridochromogenes
1.10.3.2 0.42
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.0, temperature not specified in the publication Streptomyces viridochromogenes
1.10.3.2 26.6
-
 guaiacol pH 8.5, temperature not specified in the publication Streptomyces viridochromogenes
1.10.3.2 90.5
-
 2,6-dimethoxyphenol pH 8.5, temperature not specified in the publication Streptomyces viridochromogenes