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Literature summary extracted from

  • Ganju, R.K.; Murthy, S.K.; Vithayathil, P.J.
    Purification and characterization of two cellobiohydrolases from Chaetomium thermophile var. coprophile (1989), Biochim. Biophys. Acta, 993, 266-274 .
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.4 extracellular
-
 Thermochaetoides thermophila
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.91 62000
-
 gel fitlration Thermochaetoides thermophila
3.2.1.176 42000
-
 gel fitlration Thermochaetoides thermophila

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Thermochaetoides thermophila
-
-
-
3.2.1.91 Thermochaetoides thermophila
-
-
-
3.2.1.176 Thermochaetoides thermophila
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.4 glycoprotein carbohydrate content 17%, isoform cellobiohydrolase I, 22.8%, isoform cellobiohydrolase I, respectively Thermochaetoides thermophila
3.2.1.91 glycoprotein 17% carbohydrate content Thermochaetoides thermophila
3.2.1.176 glycoprotein 22.8% carbohydrate content Thermochaetoides thermophila

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 isoforms cellobiohydrolase I and cellobiohydrolase II Thermochaetoides thermophila

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.4 culture fluid
-
 Thermochaetoides thermophila
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 microcrystaliine cellulose + H2O
-
 Thermochaetoides thermophila cellobiose + ?
-
 ?
3.2.1.4 phosphoric acid-swollen avicel + H2O
-
 Thermochaetoides thermophila cellobiose + ?
-
 ?
3.2.1.91 carboxymethylcellulose + H2O
-
 Thermochaetoides thermophila cellobiose + ?
-
 ?
3.2.1.91 lichenan + H2O
-
 Thermochaetoides thermophila ?
-
 ?
3.2.1.91 additional information enzyme degrades highly ordered crystalline forms of cellulose, i.e., cotton, microcrystalline cellulose, and Whatman CCAI cellulose, more readily than soluble polysaccharides such as lichenan, glucan, xylan and carboxymethylcellulose Thermochaetoides thermophila ?
-
 ?
3.2.1.91 phosphoric acid-swollen cellulose + H2O
-
 Thermochaetoides thermophila cellobiose + ?
-
 ?
3.2.1.176 carboxymethylcellulose + H2O
-
 Thermochaetoides thermophila cellobiose + ?
-
 ?
3.2.1.176 lichenan + H2O
-
 Thermochaetoides thermophila ?
-
 ?
3.2.1.176 additional information enzyme degrades highly ordered crystalline forms of cellulose, i.e., cotton, microcrystalline cellulose, and Whatman CCAI cellulose, more readily than soluble polysaccharides such as lichenan, glucan, xylan and carboxymethylcellulose Thermochaetoides thermophila ?
-
 ?
3.2.1.176 phosphoric acid-swollen cellulose + H2O
-
 Thermochaetoides thermophila cellobiose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 60000, isoform cellobiohydrolase I, and x * 40000, cellobiohydrolase II, SDS-PAGE Thermochaetoides thermophila
3.2.1.91 monomer 1 * 60000, SDS-PAGE Thermochaetoides thermophila
3.2.1.176 monomer 1 * 40000, SDS-PAGE Thermochaetoides thermophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 70
-
 isoform cellobiohydrolase II Thermochaetoides thermophila
3.2.1.4 75
-
 isoform cellobiohydrolase I Thermochaetoides thermophila
3.2.1.91 75
-
-
 Thermochaetoides thermophila
3.2.1.176 70
-
-
 Thermochaetoides thermophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.91 70
-
 1 h, 10% loss of activity Thermochaetoides thermophila
3.2.1.91 80
-
 1 h, 40% loss of activity Thermochaetoides thermophila
3.2.1.176 70
-
 1 h, 40% loss of activity Thermochaetoides thermophila
3.2.1.176 80
-
 1 h, 60% loss of activity Thermochaetoides thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5.8
-
 isoform cellobiohydrolase I Thermochaetoides thermophila
3.2.1.4 6.4
-
 isoform cellobiohydrolase II Thermochaetoides thermophila
3.2.1.91 5.8
-
-
 Thermochaetoides thermophila
3.2.1.176 6.4
-
-
 Thermochaetoides thermophila