Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rendon, J.; Pilet, E.; Fahs, Z.; Seduk, F.; Sylvi, L.; Hajj Chehade, M.; Pierrel, F.; Guigliarelli, B.; Magalon, A.; Grimaldi, S.
    Demethylmenaquinol is a substrate of Escherichia coli nitrate reductase A (NarGHI) and forms a stable semiquinone intermediate at the NarGHI quinol oxidation site (2015), Biochim. Biophys. Acta, 1847, 739-747 .
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.5.1 membrane
-
 Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.5.1 nitrite + demethylmenaquinone + H2O Escherichia coli
-
 nitrate + demethylmenaquinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.5.1 Escherichia coli P09152 NarG; MG1655
-
1.7.5.1 Escherichia coli P11349 NarH; MG1655
-
1.7.5.1 Escherichia coli P11350 NarI; MG1655
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.5.1 additional information structure-function relationships of quinone reactivity Escherichia coli ?
-
 ?
1.7.5.1 nitrite + demethylmenaquinone + H2O
-
 Escherichia coli nitrate + demethylmenaquinol
-
 ?
1.7.5.1 nitrite + demethylmenaquinone + H2O endogeneous demethylmenasemiquinone (DMSK) intermediates are stabilized in the enzyme. DMSK is formed at the NarGHI QD quinol oxidation site Escherichia coli nitrate + demethylmenaquinol
-
 ?
1.7.5.1 nitrite + menadione + H2O
-
 Escherichia coli nitrate + menadiol
-
 ?
1.7.5.1 nitrite + menaquinone + H2O
-
 Escherichia coli nitrate + menaquinol
-
 ?
1.7.5.1 nitrite + naphthoquinone + H2O
-
 Escherichia coli nitrate + naphthoquinol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.5.1 NarG
-
 Escherichia coli
1.7.5.1 NarGHI
-
 Escherichia coli
1.7.5.1 narH
-
 Escherichia coli
1.7.5.1 NarI
-
 Escherichia coli
1.7.5.1 nitrate reductase A
-
 Escherichia coli
1.7.5.1 quinol:nitrate oxidoreductase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.5.1 25
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.5.1 7.5
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.5.1 1,4-Naphthoquinone
-
 Escherichia coli
1.7.5.1 2-methylnaphthalene-1,4-dione
-
 Escherichia coli
1.7.5.1 demethylmenaquinone DMKH2, endogeneous demethylmenasemiquinone (DMSK) intermediates are stabilized in the enzyme Escherichia coli
1.7.5.1 heme
-
 Escherichia coli
1.7.5.1 vitamin K2
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
1.7.5.1 additional information structure-function relationships of quinone reactivity. The NarGHI catalytic activity measured with the demethylmenaquinol (DMKH2) analogue 1,4-naphthoquinol is comparable to that measured using the corresponding methylated methylmenaquinol (MKH2) analogue menadiol, kinetics, overview Escherichia coli