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Literature summary extracted from

  • Droege, K.D.; Keithly, M.E.; Sanders, C.R.; Armstrong, R.N.; Thompson, M.K.
    Structural dynamics of 15-lipoxygenase-2 via hydrogen-deuterium exchange (2017), Biochemistry, 56, 5065-5074 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.33 recombinant expression of His6-tagged isozyme 15-LOX-2 in Escherichia coli strain Rossetta 2 (DE3), coexpression with Escherichia coli YjgD protein Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.33 cytosol Ca2+ binds to the PLAT domain of 15-LOX-2, resulting in a translocation from the cytosol to the membrane Homo sapiens 5829
-
1.13.11.33 membrane Ca2+ binds to the PLAT domain of 15-LOX-2, resulting in a translocation from the cytosol to the membrane Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.33 Ca2+ Ca2+ binds to the PLAT domain of 15-LOX-2, resulting in a translocation from the cytosol to the membrane Homo sapiens
1.13.11.33 Fe2+ a non-heme iron-containing dioxygenase Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.33 arachidonate + O2 Homo sapiens
-
 (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
-
 ?
1.13.11.33 additional information Homo sapiens 15-LOX is a non-heme iron-containing dioxygenase that oxygenates polyunsaturated fatty acids (PUFA) containing cis,cis-1,4-pentadiene moieties ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.33 Homo sapiens O15296
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.33 recombinant His6-tagged isozyme 15-LOX-2 from Escherichia coli strain Rossetta 2 (DE3) by cobalt affinity chromatography, ultrafiltration, and gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.33 arachidonate + O2
-
 Homo sapiens (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
-
 ?
1.13.11.33 additional information 15-LOX is a non-heme iron-containing dioxygenase that oxygenates polyunsaturated fatty acids (PUFA) containing cis,cis-1,4-pentadiene moieties Homo sapiens ?
-
 ?
1.13.11.33 additional information specificity of phospholipid binding by 15-LOX-2, overview Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.33 15-lipoxygenase-2
-
 Homo sapiens
1.13.11.33 15-LOX-2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.33 25
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.33 7.5
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
1.13.11.33 additional information conformational dynamics of native 15-LOX-2, mass spectrometric analysis, overview Homo sapiens
1.13.11.33 physiological function isozyme 15-LOX-2 reaction in intact cells: Ca2+ concentrations in the cell increase in response to stress. The Ca2+ binds to the PLAT domain of 15-LOX-2, resulting in a translocation from the cytosol to the membrane. 15-LOX-2 then binds its substrate arachidonate and oxygenates it to form (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate, 15-HpETE. 15-HpETE is further metabolized by downstream enzymes to form lipoxins, which initiate the resolution of inflammation Homo sapiens