BRENDA - Enzyme Database

Biochemical and spectroscopic characterization of the non-heme Fe(II)- and 2-oxoglutarate-dependent ethylene-forming enzyme from Pseudomonas syringae pv. phaseolicola PK2

Martinez, S.; Hausinger, R.P.; Biochemistry 55, 5989-5999 (2016)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.13.12.19
ascorbate
required
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
ascorbate
required
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
ascorbate
1 mM used in assay conditions
Pseudomonas syringae
Cloned(Commentary)
EC Number
Commentary
Organism
1.13.12.19
gene efe, large scale expression of His6-tagged enzyme in Escherichia coli strain BL21 Gold (DE3), method optimization and evaluation
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
gene efe, large scale expression of His6-tagged enzyme in Escherichia coli strain BL21 Gold (DE3), method optimization and evaluation
Pseudomonas savastanoi pv. phaseolicola
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.20.7
0.031
-
2-oxoadipate
pH 7.5, 25°C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
0.031
-
2-oxoadipate
at pH 7.5 and 25°C
Pseudomonas syringae
1.14.20.7
0.05
-
L-arginine
with 2-oxoglutarate as cosubstrate, at pH 7.5 and 25°C
Pseudomonas syringae
1.14.20.7
0.057
-
2-oxoglutarate
pH 7.5, 25°C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
0.057
-
2-oxoglutarate
at pH 7.5 and 25°C
Pseudomonas syringae
1.14.20.7
0.071
-
L-arginine
with 2-oxoadipate as cosubstrate, at pH 7.5 and 25°C
Pseudomonas syringae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.12.19
Fe2+
dependent on, required for catalysis
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
Fe2+
dependent on, required for catalysis
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
Fe2+
required
Pseudomonas syringae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.12.19
39370
-
recombinant detagged enzyme, gel filtration
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
39370
-
recombinant detagged enzyme, gel filtration
Pseudomonas savastanoi pv. phaseolicola
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.13.12.19
2-oxoglutarate + O2
Pseudomonas savastanoi pv. phaseolicola
-
ethylene + 3 CO2 + H2O
-
-
?
1.13.12.19
2-oxoglutarate + O2
Pseudomonas savastanoi pv. phaseolicola PK2
-
ethylene + 3 CO2 + H2O
-
-
?
1.14.20.7
2-oxoglutarate + L-arginine + O2
Pseudomonas syringae
-
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
?
1.14.20.7
2-oxoglutarate + L-arginine + O2
Pseudomonas savastanoi pv. phaseolicola
-
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
?
1.14.20.7
2-oxoglutarate + L-arginine + O2
Pseudomonas savastanoi pv. phaseolicola PK2
-
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
?
1.14.20.7
2-oxoglutarate + L-arginine + O2
Pseudomonas syringae PK2
-
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.12.19
Pseudomonas savastanoi pv. phaseolicola
P32021
-
-
1.13.12.19
Pseudomonas savastanoi pv. phaseolicola PK2
P32021
-
-
1.14.20.7
Pseudomonas savastanoi pv. phaseolicola
P32021
i.e. Kudzu strain
-
1.14.20.7
Pseudomonas savastanoi pv. phaseolicola PK2
P32021
i.e. Kudzu strain
-
1.14.20.7
Pseudomonas syringae
-
pv. phaseolicola
-
1.14.20.7
Pseudomonas syringae PK2
-
pv. phaseolicola
-
Purification (Commentary)
EC Number
Commentary
Organism
1.13.12.19
recombinant His-tagged enzyme from Escherichia coli strain BL21 Gold (DE3) by nickel affinity chromatography, tag cleavage by a TEV protease mutant, and another step of nickel affinity chromatography, and dialysis of the flow through
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
Ni-NTA agarose column chromatography
Pseudomonas syringae
1.14.20.7
recombinant His-tagged enzyme from Escherichia coli strain BL21 Gold (DE3) by nickel affinity chromatography, tag cleavage by a TEV protease mutant, and another step of nickel affinity chromatography, and dialysis of the flow through
Pseudomonas savastanoi pv. phaseolicola
Reaction
EC Number
Reaction
Commentary
Organism
1.14.20.7
L-arginine + 2-oxoglutarate + O2 = succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
reaction mechanism
Pseudomonas savastanoi pv. phaseolicola
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.12.19
2-oxoglutarate + O2
-
741936
Pseudomonas savastanoi pv. phaseolicola
ethylene + 3 CO2 + H2O
-
-
-
?
1.13.12.19
2-oxoglutarate + O2
-
741936
Pseudomonas savastanoi pv. phaseolicola PK2
ethylene + 3 CO2 + H2O
-
-
-
?
1.13.12.19
additional information
cf. EC 1.14.11.34, reaction via 5-hydroxyarginine. Selected L-Arg derivatives induce ethylene formation without undergoing hydroxylation, demonstrating that ethylene production and L-Arg hydroxylation activities are not linked. Enzyme EFE utilizes the alternative 2-oxo acid 2-oxoadipate as a cosubstrate (forming glutaric acid) during the hydroxylation of L-Arg, with this reaction unlinked from ethylene formation. The amount of ethylene produced is more than twice the levels of succinate, L-DELTA1-pyrroline-5-carboxylate, or guanidine generated
741936
Pseudomonas savastanoi pv. phaseolicola
?
-
-
-
-
1.13.12.19
additional information
cf. EC 1.14.11.34, reaction via 5-hydroxyarginine. Selected L-Arg derivatives induce ethylene formation without undergoing hydroxylation, demonstrating that ethylene production and L-Arg hydroxylation activities are not linked. Enzyme EFE utilizes the alternative 2-oxo acid 2-oxoadipate as a cosubstrate (forming glutaric acid) during the hydroxylation of L-Arg, with this reaction unlinked from ethylene formation. The amount of ethylene produced is more than twice the levels of succinate, L-DELTA1-pyrroline-5-carboxylate, or guanidine generated
741936
Pseudomonas savastanoi pv. phaseolicola PK2
?
-
-
-
-
1.14.20.7
2-oxoadipate + L-arginine + O2
-
741936
Pseudomonas syringae
?
-
-
-
?
1.14.20.7
2-oxoadipate + L-arginine + O2
-
741936
Pseudomonas syringae PK2
?
-
-
-
?
1.14.20.7
2-oxoadipate + L-arginine + O2
-
741936
Pseudomonas savastanoi pv. phaseolicola
glutarate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
1.14.20.7
2-oxoadipate + L-arginine + O2
-
741936
Pseudomonas savastanoi pv. phaseolicola PK2
glutarate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
1.14.20.7
2-oxoglutarate + agmatine + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
1.14.20.7
2-oxoglutarate + agmatine + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae PK2
?
-
-
-
?
1.14.20.7
2-oxoglutarate + L-argininamide + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
1.14.20.7
2-oxoglutarate + L-arginine + O2
-
741936
Pseudomonas syringae
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
1.14.20.7
2-oxoglutarate + L-arginine + O2
-
741936
Pseudomonas savastanoi pv. phaseolicola
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
1.14.20.7
2-oxoglutarate + L-arginine + O2
100% activity
741936
Pseudomonas syringae
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
1.14.20.7
2-oxoglutarate + L-arginine + O2
-
741936
Pseudomonas savastanoi pv. phaseolicola PK2
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
1.14.20.7
2-oxoglutarate + L-arginine + O2
-
741936
Pseudomonas syringae PK2
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
1.14.20.7
2-oxoglutarate + L-arginine + O2
100% activity
741936
Pseudomonas syringae PK2
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
1.14.20.7
2-oxoglutarate + L-canavanine + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
1.14.20.7
2-oxoglutarate + L-homoarginine + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
1.14.20.7
2-oxoglutarate + L-ornithine + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
1.14.20.7
2-oxoglutarate + Ngamma-hydroxy-L-arginine + O2
15% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
1.14.20.7
additional information
no activity with 2-oxovalerate, 2-oxobutyrate, pyruvate, and oxalate
741936
Pseudomonas syringae
?
-
-
-
-
1.14.20.7
additional information
cf. EC 1.13.12.19, ethylene production. Selected L-Arg derivatives induce ethylene formation without undergoing hydroxylation, demonstrating that ethylen production and L-Arg hydroxylation activities are not linked. Enzyme EFE utilizes the alternative 2-oxo acid 2-oxoadipate as a cosubstrate (forming glutaric acid) during the hydroxylation of L-Arg, with this reaction unlinked from ethylene formation. The amount of ethylene produced is more than twice the levels of succinate, L-DELTA1-pyrroline-5-carboxylate, or guanidine generated
741936
Pseudomonas savastanoi pv. phaseolicola
?
-
-
-
-
1.14.20.7
additional information
cf. EC 1.13.12.19, ethylene production. Selected L-Arg derivatives induce ethylene formation without undergoing hydroxylation, demonstrating that ethylen production and L-Arg hydroxylation activities are not linked. Enzyme EFE utilizes the alternative 2-oxo acid 2-oxoadipate as a cosubstrate (forming glutaric acid) during the hydroxylation of L-Arg, with this reaction unlinked from ethylene formation. The amount of ethylene produced is more than twice the levels of succinate, L-DELTA1-pyrroline-5-carboxylate, or guanidine generated
741936
Pseudomonas savastanoi pv. phaseolicola PK2
?
-
-
-
-
1.14.20.7
additional information
no activity with 2-oxovalerate, 2-oxobutyrate, pyruvate, and oxalate
741936
Pseudomonas syringae PK2
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.13.12.19
monomer
1 * 40000, recombinant detagged enzyme, SDS-PAGE, 1 * 39670, recombinant detagged enzyme, mass spectrometry
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
monomer
1 * 40000, recombinant detagged enzyme, SDS-PAGE, 1 * 39670, recombinant detagged enzyme, mass spectrometry
Pseudomonas savastanoi pv. phaseolicola
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.13.12.19
25
-
assay at
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
25
-
assay at
Pseudomonas savastanoi pv. phaseolicola
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.20.7
0.0042
-
2-oxoadipate
pH 7.5, 25°C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
0.0042
-
2-oxoadipate
at pH 7.5 and 25°C
Pseudomonas syringae
1.14.20.7
0.0045
-
L-arginine
with 2-oxoadipate as cosubstrate, at pH 7.5 and 25°C
Pseudomonas syringae
1.14.20.7
0.048
-
L-arginine
at pH 7.5 and 25°C
Pseudomonas syringae
1.14.20.7
2.067
-
2-oxoglutarate
pH 7.5, 25°C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
2.07
-
2-oxoglutarate
at pH 7.5 and 25°C
Pseudomonas syringae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.12.19
6
7.5
broad optimum
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
6
7.5
broad optimum
Pseudomonas savastanoi pv. phaseolicola
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.13.12.19
6
9
over 50% of maximal activity within this range
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
6
9
over 50% of maximal activity within this range
Pseudomonas savastanoi pv. phaseolicola
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.13.12.19
ascorbate
required
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
ascorbate
required
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
ascorbate
1 mM used in assay conditions
Pseudomonas syringae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.12.19
gene efe, large scale expression of His6-tagged enzyme in Escherichia coli strain BL21 Gold (DE3), method optimization and evaluation
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
gene efe, large scale expression of His6-tagged enzyme in Escherichia coli strain BL21 Gold (DE3), method optimization and evaluation
Pseudomonas savastanoi pv. phaseolicola
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.20.7
0.031
-
2-oxoadipate
pH 7.5, 25°C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
0.031
-
2-oxoadipate
at pH 7.5 and 25°C
Pseudomonas syringae
1.14.20.7
0.05
-
L-arginine
with 2-oxoglutarate as cosubstrate, at pH 7.5 and 25°C
Pseudomonas syringae
1.14.20.7
0.057
-
2-oxoglutarate
pH 7.5, 25°C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
0.057
-
2-oxoglutarate
at pH 7.5 and 25°C
Pseudomonas syringae
1.14.20.7
0.071
-
L-arginine
with 2-oxoadipate as cosubstrate, at pH 7.5 and 25°C
Pseudomonas syringae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.12.19
Fe2+
dependent on, required for catalysis
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
Fe2+
dependent on, required for catalysis
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
Fe2+
required
Pseudomonas syringae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.12.19
39370
-
recombinant detagged enzyme, gel filtration
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
39370
-
recombinant detagged enzyme, gel filtration
Pseudomonas savastanoi pv. phaseolicola
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.13.12.19
2-oxoglutarate + O2
Pseudomonas savastanoi pv. phaseolicola
-
ethylene + 3 CO2 + H2O
-
-
?
1.13.12.19
2-oxoglutarate + O2
Pseudomonas savastanoi pv. phaseolicola PK2
-
ethylene + 3 CO2 + H2O
-
-
?
1.14.20.7
2-oxoglutarate + L-arginine + O2
Pseudomonas syringae
-
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
?
1.14.20.7
2-oxoglutarate + L-arginine + O2
Pseudomonas savastanoi pv. phaseolicola
-
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
?
1.14.20.7
2-oxoglutarate + L-arginine + O2
Pseudomonas savastanoi pv. phaseolicola PK2
-
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
?
1.14.20.7
2-oxoglutarate + L-arginine + O2
Pseudomonas syringae PK2
-
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.12.19
recombinant His-tagged enzyme from Escherichia coli strain BL21 Gold (DE3) by nickel affinity chromatography, tag cleavage by a TEV protease mutant, and another step of nickel affinity chromatography, and dialysis of the flow through
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
Ni-NTA agarose column chromatography
Pseudomonas syringae
1.14.20.7
recombinant His-tagged enzyme from Escherichia coli strain BL21 Gold (DE3) by nickel affinity chromatography, tag cleavage by a TEV protease mutant, and another step of nickel affinity chromatography, and dialysis of the flow through
Pseudomonas savastanoi pv. phaseolicola
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.12.19
2-oxoglutarate + O2
-
741936
Pseudomonas savastanoi pv. phaseolicola
ethylene + 3 CO2 + H2O
-
-
-
?
1.13.12.19
2-oxoglutarate + O2
-
741936
Pseudomonas savastanoi pv. phaseolicola PK2
ethylene + 3 CO2 + H2O
-
-
-
?
1.13.12.19
additional information
cf. EC 1.14.11.34, reaction via 5-hydroxyarginine. Selected L-Arg derivatives induce ethylene formation without undergoing hydroxylation, demonstrating that ethylene production and L-Arg hydroxylation activities are not linked. Enzyme EFE utilizes the alternative 2-oxo acid 2-oxoadipate as a cosubstrate (forming glutaric acid) during the hydroxylation of L-Arg, with this reaction unlinked from ethylene formation. The amount of ethylene produced is more than twice the levels of succinate, L-DELTA1-pyrroline-5-carboxylate, or guanidine generated
741936
Pseudomonas savastanoi pv. phaseolicola
?
-
-
-
-
1.13.12.19
additional information
cf. EC 1.14.11.34, reaction via 5-hydroxyarginine. Selected L-Arg derivatives induce ethylene formation without undergoing hydroxylation, demonstrating that ethylene production and L-Arg hydroxylation activities are not linked. Enzyme EFE utilizes the alternative 2-oxo acid 2-oxoadipate as a cosubstrate (forming glutaric acid) during the hydroxylation of L-Arg, with this reaction unlinked from ethylene formation. The amount of ethylene produced is more than twice the levels of succinate, L-DELTA1-pyrroline-5-carboxylate, or guanidine generated
741936
Pseudomonas savastanoi pv. phaseolicola PK2
?
-
-
-
-
1.14.20.7
2-oxoadipate + L-arginine + O2
-
741936
Pseudomonas syringae
?
-
-
-
?
1.14.20.7
2-oxoadipate + L-arginine + O2
-
741936
Pseudomonas syringae PK2
?
-
-
-
?
1.14.20.7
2-oxoadipate + L-arginine + O2
-
741936
Pseudomonas savastanoi pv. phaseolicola
glutarate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
1.14.20.7
2-oxoadipate + L-arginine + O2
-
741936
Pseudomonas savastanoi pv. phaseolicola PK2
glutarate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
1.14.20.7
2-oxoglutarate + agmatine + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
1.14.20.7
2-oxoglutarate + agmatine + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae PK2
?
-
-
-
?
1.14.20.7
2-oxoglutarate + L-argininamide + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
1.14.20.7
2-oxoglutarate + L-arginine + O2
-
741936
Pseudomonas syringae
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
1.14.20.7
2-oxoglutarate + L-arginine + O2
-
741936
Pseudomonas savastanoi pv. phaseolicola
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
1.14.20.7
2-oxoglutarate + L-arginine + O2
100% activity
741936
Pseudomonas syringae
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
1.14.20.7
2-oxoglutarate + L-arginine + O2
-
741936
Pseudomonas savastanoi pv. phaseolicola PK2
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
1.14.20.7
2-oxoglutarate + L-arginine + O2
-
741936
Pseudomonas syringae PK2
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
1.14.20.7
2-oxoglutarate + L-arginine + O2
100% activity
741936
Pseudomonas syringae PK2
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
1.14.20.7
2-oxoglutarate + L-canavanine + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
1.14.20.7
2-oxoglutarate + L-homoarginine + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
1.14.20.7
2-oxoglutarate + L-ornithine + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
1.14.20.7
2-oxoglutarate + Ngamma-hydroxy-L-arginine + O2
15% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
1.14.20.7
additional information
no activity with 2-oxovalerate, 2-oxobutyrate, pyruvate, and oxalate
741936
Pseudomonas syringae
?
-
-
-
-
1.14.20.7
additional information
cf. EC 1.13.12.19, ethylene production. Selected L-Arg derivatives induce ethylene formation without undergoing hydroxylation, demonstrating that ethylen production and L-Arg hydroxylation activities are not linked. Enzyme EFE utilizes the alternative 2-oxo acid 2-oxoadipate as a cosubstrate (forming glutaric acid) during the hydroxylation of L-Arg, with this reaction unlinked from ethylene formation. The amount of ethylene produced is more than twice the levels of succinate, L-DELTA1-pyrroline-5-carboxylate, or guanidine generated
741936
Pseudomonas savastanoi pv. phaseolicola
?
-
-
-
-
1.14.20.7
additional information
cf. EC 1.13.12.19, ethylene production. Selected L-Arg derivatives induce ethylene formation without undergoing hydroxylation, demonstrating that ethylen production and L-Arg hydroxylation activities are not linked. Enzyme EFE utilizes the alternative 2-oxo acid 2-oxoadipate as a cosubstrate (forming glutaric acid) during the hydroxylation of L-Arg, with this reaction unlinked from ethylene formation. The amount of ethylene produced is more than twice the levels of succinate, L-DELTA1-pyrroline-5-carboxylate, or guanidine generated
741936
Pseudomonas savastanoi pv. phaseolicola PK2
?
-
-
-
-
1.14.20.7
additional information
no activity with 2-oxovalerate, 2-oxobutyrate, pyruvate, and oxalate
741936
Pseudomonas syringae PK2
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.13.12.19
monomer
1 * 40000, recombinant detagged enzyme, SDS-PAGE, 1 * 39670, recombinant detagged enzyme, mass spectrometry
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
monomer
1 * 40000, recombinant detagged enzyme, SDS-PAGE, 1 * 39670, recombinant detagged enzyme, mass spectrometry
Pseudomonas savastanoi pv. phaseolicola
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.13.12.19
25
-
assay at
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
25
-
assay at
Pseudomonas savastanoi pv. phaseolicola
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.20.7
0.0042
-
2-oxoadipate
pH 7.5, 25°C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
0.0042
-
2-oxoadipate
at pH 7.5 and 25°C
Pseudomonas syringae
1.14.20.7
0.0045
-
L-arginine
with 2-oxoadipate as cosubstrate, at pH 7.5 and 25°C
Pseudomonas syringae
1.14.20.7
0.048
-
L-arginine
at pH 7.5 and 25°C
Pseudomonas syringae
1.14.20.7
2.067
-
2-oxoglutarate
pH 7.5, 25°C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
2.07
-
2-oxoglutarate
at pH 7.5 and 25°C
Pseudomonas syringae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.12.19
6
7.5
broad optimum
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
6
7.5
broad optimum
Pseudomonas savastanoi pv. phaseolicola
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.13.12.19
6
9
over 50% of maximal activity within this range
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
6
9
over 50% of maximal activity within this range
Pseudomonas savastanoi pv. phaseolicola
General Information
EC Number
General Information
Commentary
Organism
1.13.12.19
evolution
ethylene-forming enzyme (EFE) is a member of the mononuclear non-heme Fe(II)- and 2-oxoglutarate (2OG)-dependent oxygenase superfamily
Pseudomonas savastanoi pv. phaseolicola
1.13.12.19
physiological function
the enzyme is reported to simultaneously catalyze the conversion of 2OG into ethylene plus three CO2 and the Cdelta hydroxylation of L-arginine (L-Arg) while oxidatively decarboxylating 2OG to form succinate and carbon dioxide. The enzyme produces ethylene, a gas that is widely used as a building block in the production of various plastics, detergents, surfactants, antifreeze, solvents, and other important industrial materials. And ethylene is a plant hormone that plays an important role in growth and development. The ethylene-forming reaction is not intrinsically linked to L-Arg hydroxylation
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
evolution
ethylene-forming enzyme (EFE) is a member of the mononuclear non-heme Fe(II)- and 2-oxoglutarate (2OG)-dependent oxygenase superfamily
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
physiological function
the enzyme is reported to simultaneously catalyze the conversion of 2OG into ethylene plus three CO2 and the Cdelta hydroxylation of L-arginine (L-Arg) while oxidatively decarboxylating 2-oxoglutarate to form succinate and carbon dioxide. The enzyme produces ethylene, a gas that is widely used as a building block in the production of various plastics, detergents, surfactants, antifreeze, solvents, and other important industrial materials. And ethylene is a plant hormone that plays an important role in growth and development. The ethylene-forming reaction is not intrinsically linked to L-Arg hydroxylation
Pseudomonas savastanoi pv. phaseolicola
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.13.12.19
evolution
ethylene-forming enzyme (EFE) is a member of the mononuclear non-heme Fe(II)- and 2-oxoglutarate (2OG)-dependent oxygenase superfamily
Pseudomonas savastanoi pv. phaseolicola
1.13.12.19
physiological function
the enzyme is reported to simultaneously catalyze the conversion of 2OG into ethylene plus three CO2 and the Cdelta hydroxylation of L-arginine (L-Arg) while oxidatively decarboxylating 2OG to form succinate and carbon dioxide. The enzyme produces ethylene, a gas that is widely used as a building block in the production of various plastics, detergents, surfactants, antifreeze, solvents, and other important industrial materials. And ethylene is a plant hormone that plays an important role in growth and development. The ethylene-forming reaction is not intrinsically linked to L-Arg hydroxylation
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
evolution
ethylene-forming enzyme (EFE) is a member of the mononuclear non-heme Fe(II)- and 2-oxoglutarate (2OG)-dependent oxygenase superfamily
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
physiological function
the enzyme is reported to simultaneously catalyze the conversion of 2OG into ethylene plus three CO2 and the Cdelta hydroxylation of L-arginine (L-Arg) while oxidatively decarboxylating 2-oxoglutarate to form succinate and carbon dioxide. The enzyme produces ethylene, a gas that is widely used as a building block in the production of various plastics, detergents, surfactants, antifreeze, solvents, and other important industrial materials. And ethylene is a plant hormone that plays an important role in growth and development. The ethylene-forming reaction is not intrinsically linked to L-Arg hydroxylation
Pseudomonas savastanoi pv. phaseolicola
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.14.20.7
0.134
-
2-oxoadipate
pH 7.5, 25°C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
0.97
-
L-arginine
at pH 7.5 and 25°C
Pseudomonas syringae
1.14.20.7
36.26
-
2-oxoglutarate
pH 7.5, 25°C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
36.33
-
2-oxoglutarate
at pH 7.5 and 25°C
Pseudomonas syringae
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.14.20.7
0.134
-
2-oxoadipate
pH 7.5, 25°C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
0.97
-
L-arginine
at pH 7.5 and 25°C
Pseudomonas syringae
1.14.20.7
36.26
-
2-oxoglutarate
pH 7.5, 25°C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
1.14.20.7
36.33
-
2-oxoglutarate
at pH 7.5 and 25°C
Pseudomonas syringae