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Literature summary extracted from

  • Han, A.R.; Kim, M.J.; Kwak, G.H.; Son, J.; Hwang, K.Y.; Kim, H.Y.
    Essential role of the linker region in the higher catalytic efficiency of a bifunctional MsrA-MsrB fusion protein (2016), Biochemistry, 55, 5117-5127 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.11
-
 Treponema denticola
1.8.4.12
-
 Treponema denticola

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.4.11 to 2.3 A resolution, in presence and absence of dithiothreitol Treponema denticola
1.8.4.12 to 2.3 A resolution, in presence and absence of dithiothreitol Treponema denticola

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.11 C285S kcat value is similar to that of wild-type, while its Km value is slightly higher than that of the wild type Treponema denticola
1.8.4.11 D164A catalytic efficiency is about 120% of wild-type Treponema denticola
1.8.4.11 E165A catalytic efficiency is similar to wild-type Treponema denticola
1.8.4.11 Y163A catalytic efficiency is about 60% of wild-type Treponema denticola
1.8.4.11 Y163A/E165A catalytic efficiency is about 8% of wild-type Treponema denticola
1.8.4.12 C11S catalytic efficiency is about 140% of wild-type Treponema denticola
1.8.4.12 D164A catalytic efficiency is about 80% of wild-type Treponema denticola
1.8.4.12 E165A catalytic efficiency is about 65% to wild-type Treponema denticola
1.8.4.12 Y163A catalytic efficiency is about 20% of wild-type Treponema denticola
1.8.4.12 Y163A/E165A catalytic efficiency is about 17% of wild-type Treponema denticola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.11 0.14
-
 dabsyl-L-methionine (S)-S-oxide mutant D164A, pH 7.5, 37°C Treponema denticola
1.8.4.11 0.15
-
 dabsyl-L-methionine (S)-S-oxide mutant E165A, pH 7.5, 37°C Treponema denticola
1.8.4.11 0.17
-
 dabsyl-L-methionine (S)-S-oxide mutant Y163A, pH 7.5, 37°C Treponema denticola
1.8.4.11 0.18
-
 dabsyl-L-methionine (S)-S-oxide wild-type, pH 7.5, 37°C Treponema denticola
1.8.4.11 0.3
-
 dabsyl-L-methionine (S)-S-oxide isolated MrsA domain, pH 7.5, 37°C Treponema denticola
1.8.4.11 0.3
-
 dabsyl-L-methionine (S)-S-oxide mutant C285S, pH 7.5, 37°C Treponema denticola
1.8.4.11 1.03
-
 dabsyl-L-methionine (S)-S-oxide mutant Y163A//E165A, pH 7.5, 37°C Treponema denticola
1.8.4.12 0.03
-
 dabsyl-L-methionine (R)-S-oxide mutant C11S, pH 7.5, 37°C Treponema denticola
1.8.4.12 0.04
-
 dabsyl-L-methionine (R)-S-oxide mutant D164A, pH 7.5, 37°C Treponema denticola
1.8.4.12 0.04
-
 dabsyl-L-methionine (R)-S-oxide mutant E165A, pH 7.5, 37°C Treponema denticola
1.8.4.12 0.05
-
 dabsyl-L-methionine (R)-S-oxide wild-type, pH 7.5, 37°C Treponema denticola
1.8.4.12 0.05
-
 dabsyl-L-methionine (R)-S-oxide mutant Y163A, pH 7.5, 37°C Treponema denticola
1.8.4.12 0.06
-
 dabsyl-L-methionine (R)-S-oxide mutant Y163A//E165A, pH 7.5, 37°C Treponema denticola
1.8.4.12 0.085
-
 dabsyl-L-methionine (R)-S-oxide isolated MrsB domain, pH 7.5, 37°C Treponema denticola

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.11 Treponema denticola Q73PT7 bifunctional methionine sulfoxide reductase AB, cf. EC 1.8.1.12
-
1.8.4.11 Treponema denticola ATCC 35405 Q73PT7 bifunctional methionine sulfoxide reductase AB, cf. EC 1.8.1.12
-
1.8.4.12 Treponema denticola Q73PT7 bifunctional methionine sulfoxide reductase AB, cf. EC 1.8.1.11
-
1.8.4.12 Treponema denticola ATCC 35405 Q73PT7 bifunctional methionine sulfoxide reductase AB, cf. EC 1.8.1.11
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.11 dabsyl-L-methionine (S)-S-oxide + dithiothreitol
-
 Treponema denticola dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?
1.8.4.11 dabsyl-L-methionine (S)-S-oxide + dithiothreitol
-
 Treponema denticola ATCC 35405 dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?
1.8.4.12 dabsyl-L-methionine (R)-S-oxide + dithiothreitol
-
 Treponema denticola dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?
1.8.4.12 dabsyl-L-methionine (R)-S-oxide + dithiothreitol
-
 Treponema denticola ATCC 35405 dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.4.11 51.3
-
 wild-type, melting temperature Treponema denticola
1.8.4.11 53.6
-
 isolated MrsA domain, melting temperature Treponema denticola
1.8.4.12 51.3
-
 wild-type, melting temperature Treponema denticola
1.8.4.12 53.6
-
 isolated MrsA domain, melting temperature Treponema denticola

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.4.11 0.026
-
 dabsyl-L-methionine (S)-S-oxide isolated MrsA domain, pH 7.5, 37°C Treponema denticola
1.8.4.11 0.045
-
 dabsyl-L-methionine (S)-S-oxide mutant Y163A//E165A, pH 7.5, 37°C Treponema denticola
1.8.4.11 0.068
-
 dabsyl-L-methionine (S)-S-oxide mutant Y163A, pH 7.5, 37°C Treponema denticola
1.8.4.11 0.096
-
 dabsyl-L-methionine (S)-S-oxide mutant E165A, pH 7.5, 37°C Treponema denticola
1.8.4.11 0.108
-
 dabsyl-L-methionine (S)-S-oxide mutant C285S, pH 7.5, 37°C Treponema denticola
1.8.4.11 0.11
-
 dabsyl-L-methionine (S)-S-oxide mutant D164A, pH 7.5, 37°C Treponema denticola
1.8.4.11 0.12
-
 dabsyl-L-methionine (S)-S-oxide wild-type, pH 7.5, 37°C Treponema denticola
1.8.4.12 0.004
-
 dabsyl-L-methionine (R)-S-oxide isolated MrsB domain, pH 7.5, 37°C Treponema denticola
1.8.4.12 0.025
-
 dabsyl-L-methionine (R)-S-oxide mutant Y163A//E165A, pH 7.5, 37°C Treponema denticola
1.8.4.12 0.036
-
 dabsyl-L-methionine (R)-S-oxide mutant Y163A, pH 7.5, 37°C Treponema denticola
1.8.4.12 0.068
-
 dabsyl-L-methionine (R)-S-oxide mutant E165A, pH 7.5, 37°C Treponema denticola
1.8.4.12 0.083
-
 dabsyl-L-methionine (R)-S-oxide mutant D164A, pH 7.5, 37°C Treponema denticola
1.8.4.12 0.102
-
 dabsyl-L-methionine (R)-S-oxide mutant C11S, pH 7.5, 37°C Treponema denticola
1.8.4.12 0.116
-
 dabsyl-L-methionine (R)-S-oxide wild-type, pH 7.5, 37°C Treponema denticola

General Information

EC Number General Information Comment Organism
1.8.4.11 physiological function methionine sulfoxide reductase A (MsrA, EC 1.8.1.11) and B (MsrB, EC 1.8.1.12) are present as a fusion form. The catalytic efficiency of both MsrA and MsrB increases after fusion of the domains and the linker region (iloop) that connects MsrA and MsrB is required for the higher catalytic efficiency of the fusion protein. The iloop mainly interacts with MsrB via hydrogen bonds. The iloop-MsrB interactions are critical to MsrB and MsrA activities Treponema denticola
1.8.4.12 physiological function methionine sulfoxide reductase A (MsrA, EC 1.8.1.11) and B (MsrB, EC 1.8.1.12) are present as a fusion form. The catalytic efficiency of both MsrA and MsrB increases after fusion of the domains and the linker region (iloop) that connects MsrA and MsrB is required for the higher catalytic efficiency of the fusion protein. The iloop mainly interacts with MsrB via hydrogen bonds. The iloop-MsrB interactions are critical to MsrB and MsrA activities Treponema denticola

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.4.11 0.044
-
 dabsyl-L-methionine (S)-S-oxide mutant Y163A//E165A, pH 7.5, 37°C Treponema denticola
1.8.4.11 0.4
-
 dabsyl-L-methionine (S)-S-oxide mutant Y163A, pH 7.5, 37°C Treponema denticola
1.8.4.11 0.64
-
 dabsyl-L-methionine (S)-S-oxide mutant E165A, pH 7.5, 37°C Treponema denticola
1.8.4.11 0.667
-
 dabsyl-L-methionine (S)-S-oxide wild-type, pH 7.5, 37°C Treponema denticola
1.8.4.11 0.786
-
 dabsyl-L-methionine (S)-S-oxide mutant D164A, pH 7.5, 37°C Treponema denticola
1.8.4.11 87
-
 dabsyl-L-methionine (S)-S-oxide isolated MrsA domain, pH 0.75, 37°C Treponema denticola
1.8.4.11 360
-
 dabsyl-L-methionine (S)-S-oxide mutant C285S, pH 7.5, 37°C Treponema denticola
1.8.4.12 0.005
-
 dabsyl-L-methionine (R)-S-oxide isolated MrsB domain, pH 7.5, 37°C Treponema denticola
1.8.4.12 0.42
-
 dabsyl-L-methionine (R)-S-oxide mutant Y163A//E165A, pH 7.5, 37°C Treponema denticola
1.8.4.12 0.72
-
 dabsyl-L-methionine (R)-S-oxide mutant Y163A, pH 7.5, 37°C Treponema denticola
1.8.4.12 1.7
-
 dabsyl-L-methionine (R)-S-oxide mutant E165A, pH 7.5, 37°C Treponema denticola
1.8.4.12 2.08
-
 dabsyl-L-methionine (R)-S-oxide mutant D164A, pH 7.5, 37°C Treponema denticola
1.8.4.12 2.32
-
 dabsyl-L-methionine (R)-S-oxide wild-type, pH 7.5, 37°C Treponema denticola
1.8.4.12 3.4
-
 dabsyl-L-methionine (R)-S-oxide mutant C11S, pH 7.5, 37°C Treponema denticola