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Literature summary extracted from

  • Gasselhuber, B.; Graf, M.M.; Jakopitsch, C.; Zamocky, M.; Nicolussi, A.; Furtmueller, P.G.; Oostenbrink, C.; Carpena, X.; Obinger, C.
    Interaction with the redox cofactor MYW and functional role of a mobile arginine in eukaryotic catalase-peroxidase (2016), Biochemistry, 55, 3528-3541 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.21 expressed in Escherichia coli BL21(DE3) Star cells Pyricularia grisea

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.21 sitting drop vapor diffusion method, using 15% (w/v) PEG 4000 and 0.1 M sodium acetate (pH 4.6) Pyricularia grisea

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.21 R461A the mutation slightly increases the thermal stability but does not alter the active site architecture or the kinetics of cyanide binding. However, the variant loses the wild type-typical optimum of catalase activity at pH 5.3 but exhibits a broad plateau between pH 4.5 and 7.5 Pyricularia grisea

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.21 Pyricularia grisea A4QUT2
-
-
1.11.1.21 Pyricularia grisea 70-15 A4QUT2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.21 chelating Sepharose column chromatography, and hydroxyapatite column chromatography Pyricularia grisea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.21 2 H2O2
-
 Pyricularia grisea O2 + H2O
-
 ?
1.11.1.21 2 H2O2
-
 Pyricularia grisea 70-15 O2 + H2O
-
 ?
1.11.1.21 guaiacol + H2O2
-
 Pyricularia grisea tetraguaiacol + H2O
-
 ?
1.11.1.21 guaiacol + H2O2
-
 Pyricularia grisea 70-15 tetraguaiacol + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.21 KatG2
-
 Pyricularia grisea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.21 53
-
 melting temperatures are at 53 and 63°C Pyricularia grisea
1.11.1.21 63
-
 melting temperatures are at 53 and 63°C Pyricularia grisea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.21 270
-
 H2O2 mutant enzyme R461A, at pH 5.5 and 30°C Pyricularia grisea
1.11.1.21 6450
-
 H2O2 wild type enzyme, at pH 5.3 and 30°C Pyricularia grisea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.21 5.3
-
-
 Pyricularia grisea