EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.33 | gene loxA, recombinant expression of N-terminally His6-tagged enzyme LoxA in Escherichia coli strain BL21(DE3) | Pseudomonas aeruginosa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.33 | 1-(4-tert-butylphenyl)-4-[4-(diphenylmethoxy)piperidin-1-yl]butan-1-one | - |
Pseudomonas aeruginosa | |
1.13.11.33 | 1-[1-[4,4-bis(4-fluorophenyl)butyl]piperidin-4-yl]-1,3-dihydro-2H-benzimidazol-2-one | - |
Pseudomonas aeruginosa | |
1.13.11.33 | 1-[2-[bis(4-fluorophenyl)methoxy]ethyl]-4-(3-phenylpropyl)piperazine | - |
Pseudomonas aeruginosa | |
1.13.11.33 | 1-[4-[4-([[5-(3-chlorophenyl)furan-2-yl]methyl]amino)phenyl]piperazin-1-yl]ethan-1-one | - |
Pseudomonas aeruginosa | |
1.13.11.33 | 1-[bis(4-fluorophenyl)methyl]-4-[(2E)-3-phenylprop-2-en-1-yl]piperazine | - |
Pseudomonas aeruginosa | |
1.13.11.33 | 2-(4-chlorophenyl)-5-cyclohexyl-1,3,4-oxadiazole | - |
Pseudomonas aeruginosa | |
1.13.11.33 | 2-[4-[(1Z)-1,2-diphenylbut-1-en-1-yl]phenoxy]-N,N-dimethylethan-1-amine | - |
Pseudomonas aeruginosa | |
1.13.11.33 | 4,4'-(2,3-dimethylbutane-1,4-diyl)di(benzene-1,2-diol) | - |
Pseudomonas aeruginosa | |
1.13.11.33 | 4-(3,4-dichlorophenyl)-N-methyl-1,2,3,4-tetrahydronaphthalen-1-amine | - |
Pseudomonas aeruginosa | |
1.13.11.33 | 4-[[5-(naphthalen-1-yl)-1,3,4-oxadiazol-2-yl]sulfanyl]but-2-yn-1-yl benzoate | - |
Pseudomonas aeruginosa | |
1.13.11.33 | 6-[4-([1,1'-biphenyl]-2-yl)piperazin-1-yl]-N-(1,2,3,4-tetrahydronaphthalen-1-yl)hexanamide | - |
Pseudomonas aeruginosa | |
1.13.11.33 | 7-cyclopentyl-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-d]pyrimidin-4-amine | - |
Pseudomonas aeruginosa | |
1.13.11.33 | 8-[4,4-bis(4-fluorophenyl)butyl]-1-phenyl-1,3,8-triazaspiro[4.5]decan-4-one | - |
Pseudomonas aeruginosa | |
1.13.11.33 | methyl 11,17-dimethoxy-18-[(3,4,5-trimethoxybenzoyl)oxy]yohimban-16-carboxylate | - |
Pseudomonas aeruginosa | |
1.13.11.33 | additional information | inhibitor library screening, none selectively targets LoxA over the human LOX isozymes, overview | Pseudomonas aeruginosa | |
1.13.11.33 | N-[(5-bromo-8-hydroxy-5,6,7,8-tetrahydroquinolin-7-yl)(thiophen-2-yl)methyl]acetamide | - |
Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.33 | additional information | - |
additional information | Michaelis-Menten steady-state kinetics | Pseudomonas aeruginosa | |
1.13.11.33 | 0.007 | - |
linoleic acid | pH 6.5, 22°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | |
1.13.11.33 | 0.012 | - |
arachidonate | pH 6.5-7.0, 22°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | |
1.13.11.33 | 0.013 | - |
arachidonate | pH 7.5, 22°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.13.11.33 | extracellular | the enzyme is secreted | Pseudomonas aeruginosa | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.33 | arachidonate + O2 | Pseudomonas aeruginosa | - |
(5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.33 | Pseudomonas aeruginosa | Q9I4G8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.33 | recombinant N-terminally His6-tagged enzyme LoxA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.33 | alpha-linoleic acid + O2 | 72% activity compared to arachidonate | Pseudomonas aeruginosa | ? | - |
? | |
1.13.11.33 | arachidonate + O2 | - |
Pseudomonas aeruginosa | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate | - |
? | |
1.13.11.33 | arachidonate + O2 | best substrate | Pseudomonas aeruginosa | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate | - |
? | |
1.13.11.33 | dihomo-gamma-linolenic acid + O2 | 44% activity compared to arachidonate | Pseudomonas aeruginosa | ? | - |
? | |
1.13.11.33 | docosahexaenoic acid + O2 | 72% activity compared to arachidonate | Pseudomonas aeruginosa | ? | - |
? | |
1.13.11.33 | eicosadienoic acid + O2 | 33% activity compared to arachidonate | Pseudomonas aeruginosa | ? | - |
? | |
1.13.11.33 | eicosapentanoic acid + O2 | 61% activity compared to arachidonate | Pseudomonas aeruginosa | ? | - |
? | |
1.13.11.33 | gamma-linolenic acid + O2 | 44% activity compared to arachidonate | Pseudomonas aeruginosa | ? | - |
? | |
1.13.11.33 | linoleic acid + O2 | 78% activity compared to arachidonate | Pseudomonas aeruginosa | (9Z,11E)-(13S)-13-hydroperoxy-octadeca-9,11-dienoate | - |
? | |
1.13.11.33 | additional information | enzyme LoxA is capable of efficiently catalyzing the peroxidation of a broad range of free fatty acid substrates, e.g. arachidonate and linoleate with high positional specificity, indicating a 15-LOX, its mechanism includes hydrogen atom abstraction. LoxA also does not react with 5- or 15-15-hydroperoxyicosatetraenoates, or phosphoester fatty acids | Pseudomonas aeruginosa | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.33 | 15-lipoxygenase | - |
Pseudomonas aeruginosa |
1.13.11.33 | LoxA | - |
Pseudomonas aeruginosa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.11.33 | 22 | - |
assay at | Pseudomonas aeruginosa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.33 | 2 | 8 | linoleic acid | pH 6.5, 22°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | |
1.13.11.33 | 86 | - |
arachidonate | pH 7.5, 22°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | |
1.13.11.33 | 157 | - |
arachidonate | pH 7.0, 22°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | |
1.13.11.33 | 181 | - |
arachidonate | pH 6.5, 22°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.33 | 6.5 | - |
assay at | Pseudomonas aeruginosa |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.13.11.33 | 0.0041 | - |
pH 6.5, 2°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | 8-[4,4-bis(4-fluorophenyl)butyl]-1-phenyl-1,3,8-triazaspiro[4.5]decan-4-one | |
1.13.11.33 | 0.0045 | - |
pH 6.5, 2°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | 1-[bis(4-fluorophenyl)methyl]-4-[(2E)-3-phenylprop-2-en-1-yl]piperazine | |
1.13.11.33 | 0.0058 | - |
pH 6.5, 2°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | 1-[1-[4,4-bis(4-fluorophenyl)butyl]piperidin-4-yl]-1,3-dihydro-2H-benzimidazol-2-one | |
1.13.11.33 | 0.0067 | - |
pH 6.5, 2°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | 2-[4-[(1Z)-1,2-diphenylbut-1-en-1-yl]phenoxy]-N,N-dimethylethan-1-amine | |
1.13.11.33 | 0.008 | - |
pH 6.5, 2°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | 1-[2-[bis(4-fluorophenyl)methoxy]ethyl]-4-(3-phenylpropyl)piperazine | |
1.13.11.33 | 0.0101 | - |
pH 6.5, 2°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | 1-(4-tert-butylphenyl)-4-[4-(diphenylmethoxy)piperidin-1-yl]butan-1-one | |
1.13.11.33 | 0.011 | - |
pH 6.5, 2°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | 4,4'-(2,3-dimethylbutane-1,4-diyl)di(benzene-1,2-diol) | |
1.13.11.33 | 0.0114 | - |
pH 6.5, 2°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | 6-[4-([1,1'-biphenyl]-2-yl)piperazin-1-yl]-N-(1,2,3,4-tetrahydronaphthalen-1-yl)hexanamide | |
1.13.11.33 | 0.0131 | - |
pH 6.5, 2°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | 4-(3,4-dichlorophenyl)-N-methyl-1,2,3,4-tetrahydronaphthalen-1-amine | |
1.13.11.33 | 0.0138 | - |
pH 6.5, 2°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | methyl 11,17-dimethoxy-18-[(3,4,5-trimethoxybenzoyl)oxy]yohimban-16-carboxylate | |
1.13.11.33 | 0.0162 | - |
pH 6.5, 2°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | 7-cyclopentyl-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-d]pyrimidin-4-amine | |
1.13.11.33 | 0.02 | - |
pH 6.5, 2°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | 4-[[5-(naphthalen-1-yl)-1,3,4-oxadiazol-2-yl]sulfanyl]but-2-yn-1-yl benzoate | |
1.13.11.33 | 0.046 | - |
pH 6.5, 2°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | 1-[4-[4-([[5-(3-chlorophenyl)furan-2-yl]methyl]amino)phenyl]piperazin-1-yl]ethan-1-one | |
1.13.11.33 | 0.078 | - |
pH 6.5, 2°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | 2-(4-chlorophenyl)-5-cyclohexyl-1,3,4-oxadiazole | |
1.13.11.33 | 0.1 | - |
pH 6.5, 2°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | N-[(5-bromo-8-hydroxy-5,6,7,8-tetrahydroquinolin-7-yl)(thiophen-2-yl)methyl]acetamide |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.13.11.33 | Pseudomonas aeruginosa | the level of LoxA expression is increased when Pseudomonas aeruginosa undergoes the transition to a biofilm mode of growth | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.13.11.33 | physiological function | enzyme LoxA is a poor catalyst against phosphoester fatty acids, suggesting that LoxA is not involved in membrane decomposition. LoxA also does not react with 5- or 15-HETEs, indicating poor involvement in lipoxin production. The level of LoxA expression is increased when Pseudomonas aeruginosa undergoes the transition to a biofilm mode of growth, but LoxA is not required for biofilm growth on abiotic surfaces. LoxA does appear to be required for biofilm growth in association with the host airway epithelium, suggesting a role for LoxA in mediating bacterium-host interactions during colonization | Pseudomonas aeruginosa |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.33 | 0.0038 | - |
linoleic acid | pH 6.5, 22°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | |
1.13.11.33 | 0.0066 | - |
arachidonate | pH 7.5, 22°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | |
1.13.11.33 | 0.013 | - |
arachidonate | pH 7.0, 22°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa | |
1.13.11.33 | 0.016 | - |
arachidonate | pH 6.5, 22°C, recombinant His6-tagged enzyme | Pseudomonas aeruginosa |