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Literature summary extracted from

  • Joseph, E.; Le, C.Q.; Nguyen, T.; Oyugi, M.; Hossain, M.S.; Foss, F.W.; Johnson-Winters, K.
    Evidence of negative cooperativity and half-site reactivity within an F420-dependent enzyme kinetic analysis of F420H2 NADP+ oxidoreductase (2016), Biochemistry, 55, 1082-1090 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.40 cloning and recombinant expression in Escherichia coli strain C41(DE3) Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.40 additional information
-
 additional information the enzyme shows half-site reactivity and negative cooperativity (Koshland-Nemethy-Filmer model) in the reversible reduction of NADP+ through the transfer of a hydride from the reduced F420 cofactor, steady-state kinetic analysis revealing classical Michaelis-Menten kinetics with varying concentrations of the F420 redox moiety, and non-Michaelis-Menten kinetic behavior when NADPH is varied. Pre-steady-state, stopped flow, Single-turnover, and steady-state kinetics, detailed overview Archaeoglobus fulgidus
1.5.1.40 4
-
 oxidized coenzyme F420 pH 6.5, 22°C, recombinant enzyme Archaeoglobus fulgidus
1.5.1.40 61.6
-
 NADPH pH 6.5, 22°C, recombinant enzyme Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.40 reduced coenzyme F420 + NADP+ Archaeoglobus fulgidus
-
 oxidized coenzyme F420 + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.40 Archaeoglobus fulgidus O29370
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.40 recombinant enzyme from cell-free extracts of Escherichia coli strain C41(DE3) by heat treatment at 90°C for 30 min, ammonium sulfate fractionation with addition of 0.05% polyethylenimine, followed by an ion exchange chromatography, ultrafiltration, and gel filtration Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.40 reduced coenzyme F420 + NADP+
-
 Archaeoglobus fulgidus oxidized coenzyme F420 + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.5.1.40 F420H2:NADP+ oxidoreductase
-
 Archaeoglobus fulgidus
1.5.1.40 Fno
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.40 22
-
 assay at Archaeoglobus fulgidus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.40 5.27
-
 oxidized coenzyme F420 pH 6.5, 22°C, recombinant enzyme Archaeoglobus fulgidus
1.5.1.40 5.41
-
 NADPH pH 6.5, 22°C, recombinant enzyme Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.40 6.5
-
 assay at Archaeoglobus fulgidus

General Information

EC Number General Information Comment Organism
1.5.1.40 physiological function half-site reactivity and negative cooperativity involving the important F420 cofactor-dependent enzyme. F420H2:NADP+ oxidoreductase (Fno), an F420 cofactor-dependent enzyme that catalyzes the reversible reduction of NADP+ through the transfer of a hydride from the reduced F420 cofactor. Fno may be a functional regulatory enzyme Archaeoglobus fulgidus