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Literature summary extracted from

  • Uchida, T.; Sasaki, M.; Tanaka, Y.; Ishimori, K.
    A dye-decolorizing peroxidase from Vibrio cholerae (2015), Biochemistry, 54, 6610-6621 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.19 expressed in Escherichia coli BL21(DE3) cells Vibrio cholerae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.19 sitting drop vapor diffusion method, using 0.2 M potassium thiocyanate and 20% (w/v) PEG 3000 Vibrio cholerae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.19 0.01
-
 guaiacol at pH 8.0 and 25°C Vibrio cholerae
1.11.1.19 0.05
-
 Reactive Blue 19 at pH 8.0 and 25°C Vibrio cholerae
1.11.1.19 0.18
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 8.0 and 25°C Vibrio cholerae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.19 70000
-
 gel filtration Vibrio cholerae

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.19 Vibrio cholerae Q9KQ59
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.19 HisTrap column chromatography and Superdex 200 gel filtration Vibrio cholerae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.19 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Vibrio cholerae ?
-
 ?
1.11.1.19 guaiacol + H2O2 + H+
-
 Vibrio cholerae ?
-
 ?
1.11.1.19 Reactive Blue 19 + H2O2 + H+
-
 Vibrio cholerae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.19 homodimer 2 * 35000, SDS-PAGE Vibrio cholerae

Synonyms

EC Number Synonyms Comment Organism
1.11.1.19 dye-decolorizing peroxidase
-
 Vibrio cholerae
1.11.1.19 DyP
-
 Vibrio cholerae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.19 0.027
-
 guaiacol at pH 8.0 and 25°C Vibrio cholerae
1.11.1.19 1.3
-
 Reactive Blue 19 at pH 8.0 and 25°C Vibrio cholerae
1.11.1.19 500
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 8.0 and 25°C Vibrio cholerae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.19 4
-
-
 Vibrio cholerae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.19 26
-
 Reactive Blue 19 at pH 8.0 and 25°C Vibrio cholerae