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Literature summary extracted from

  • Hofbauer, S.; Gruber, C.; Pirker, K.F.; Suendermann, A.; Schaffner, I.; Jakopitsch, C.; Oostenbrink, C.; Furtmueller, P.G.; Obinger, C.
    Transiently produced hypochlorite is responsible for the irreversible inhibition of chlorite dismutase (2014), Biochemistry, 53, 3145-3157 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.49 additional information presence of methionine can partly rescue inactivation during the reaction Nitrospira defluvii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.49 expresssion in Escherichia coli Nitrospira defluvii

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.49 R173A mutation increases the extent of irreversible inactivation. In the presence of the hypochlorite traps methionine, monochlorodimedone, and 2-[6-(4-aminophenoxy)-3-oxo-3H-xanthen-9-yl]benzoic acid, the extent of chlorite degradation and release of molecular oxygen is significantly increased, whereas heme bleaching and oxidative modifications of the protein are suppressed Nitrospira defluvii
1.13.11.49 R173K mutation increases the extent of irreversible inactivation. In the presence of the hypochlorite traps methionine, monochlorodimedone, and 2-[6-(4-aminophenoxy)-3-oxo-3H-xanthen-9-yl]benzoic acid, the extent of chlorite degradation and release of molecular oxygen is significantly increased, whereas heme bleaching and oxidative modifications of the protein are suppressed Nitrospira defluvii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.49 0.069
-
 Chlorite wild-type, pH 7.0, 30°C Nitrospira defluvii
1.13.11.49 0.09
-
 Chlorite mutant R173A, pH 7.0, 30°C Nitrospira defluvii
1.13.11.49 0.898
-
 Chlorite mutant R173K, pH 7.0, 30°C Nitrospira defluvii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.49 chlorite Nitrospira defluvii
-
 chloride + O2
-
 ?
1.13.11.49 additional information Nitrospira defluvii upon reduction of chlorite, hypochlorite is formed and kept in the reaction sphere for recombination with the oxoiron(IV) group of Compound I. Approximately one molecule of HOCl per 100 full cycles escapes and reacts with both the prosthetic group and the protein moiety, leading to irreversible inactivation of Cld is observed which is more pronounced with an increase in pH. HOCl traps like methionine can rescue the enzyme from inactivation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.49 Nitrospira defluvii B3U4H7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.49 chlorite
-
 Nitrospira defluvii chloride + O2
-
 ?
1.13.11.49 additional information upon reduction of chlorite, hypochlorite is formed and kept in the reaction sphere for recombination with the oxoiron(IV) group of Compound I. Approximately one molecule of HOCl per 100 full cycles escapes and reacts with both the prosthetic group and the protein moiety, leading to irreversible inactivation of Cld is observed which is more pronounced with an increase in pH. HOCl traps like methionine can rescue the enzyme from inactivation Nitrospira defluvii ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.49 CLD
-
 Nitrospira defluvii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.49 2.8
-
 Chlorite mutant R173A, pH 7.0, 30°C Nitrospira defluvii
1.13.11.49 14
-
 Chlorite mutant R173K, pH 7.0, 30°C Nitrospira defluvii
1.13.11.49 43
-
 Chlorite wild-type, pH 7.0, 30°C Nitrospira defluvii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.49 additional information
-
 KM value is independent of pH Nitrospira defluvii
1.13.11.49 4.5
-
 mutants R173A and R173K Nitrospira defluvii
1.13.11.49 5.5
-
 wild-type Nitrospira defluvii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.49 15
-
 Chlorite mutant R173K, pH 7.0, 30°C Nitrospira defluvii
1.13.11.49 31
-
 Chlorite mutant R173A, pH 7.0, 30°C Nitrospira defluvii
1.13.11.49 620
-
 Chlorite wild-type, pH 7.0, 30°C Nitrospira defluvii