EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.1.46 | additional information | immobilization of the purified enzyme on seven types of mesoporous silica (MPS), whose pores are from 2.4 to 31.2 nm sized, avaluatio of the enzyme activity in oxidation of formaldehyde, method overview. Enhancement of catalytic activity is obtained by immobilizing onto MPS, whose pore (mesopore) size is similar to the molecular size of enzyme FDH. FDH immobilized on phenyl-functionalized MPS-4 (MPS-4-Ph) has higher activity than FDH immobilized on non-functionalized one. Immobilized FDH on MPS-4-Ph is active for low formaldehyde concentration form 6.0 M and more sensitive than conventional formaldehyde detectors. The high-order structure of the FDH does not alter upon binding to the non-functionalized MPS surface. But FDH immobilized on functionalized-MPS is changed by hydrophobic interaction or covalent binding | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.46 | SDS | 1 mM, over 50% loss of activity for the free enzyme, about 10% loss of activity for the immobilized enzymes | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.46 | 0.438 | - |
formaldehyde | enzyme immobilized on phenyl-functionalized MPS-4, pH 7.0, 22° | Pseudomonas putida | |
1.2.1.46 | 0.926 | - |
formaldehyde | pH 7.0, 22°C, free enzyme | Pseudomonas putida | |
1.2.1.46 | 2.84 | - |
formaldehyde | enzyme immobilized on MPS-4, pH 7.0, 22° | Pseudomonas putida | |
1.2.1.46 | 10.6 | - |
formaldehyde | enzyme immobilized on methyl-functionalized MPS-4, pH 7.0, 22° | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.46 | formaldehyde + NAD+ + H2O | Pseudomonas putida | - |
formate + NADH + 2 H+ | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.2.1.46 | Methanol | 5%, high loss of activity for the free enzyme, while the immobilzed enzymes are stable | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.46 | Pseudomonas putida | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.2.1.46 | commercial preparation | - |
Pseudomonas putida | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.46 | formaldehyde + NAD+ + H2O | - |
Pseudomonas putida | formate + NADH + 2 H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.46 | FDH | - |
Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.46 | 22 | - |
assay at room temperature | Pseudomonas putida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.46 | 0.136 | - |
formaldehyde | enzyme immobilized on MPS-4, pH 7.0, 22° | Pseudomonas putida | |
1.2.1.46 | 0.177 | - |
formaldehyde | enzyme immobilized on phenyl-functionalized MPS-4, pH 7.0, 22° | Pseudomonas putida | |
1.2.1.46 | 0.247 | - |
formaldehyde | enzyme immobilized on methyl-functionalized MPS-4, pH 7.0, 22° | Pseudomonas putida | |
1.2.1.46 | 0.48 | - |
formaldehyde | pH 7.0, 22°C, free enzyme | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.46 | 7 | - |
assay at | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.46 | NAD+ | - |
Pseudomonas putida |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.46 | 0.023 | - |
formaldehyde | enzyme immobilized on methyl-functionalized MPS-4, pH 7.0, 22° | Pseudomonas putida | |
1.2.1.46 | 0.047 | - |
formaldehyde | enzyme immobilized on MPS-4, pH 7.0, 22° | Pseudomonas putida | |
1.2.1.46 | 0.404 | - |
formaldehyde | enzyme immobilized on phenyl-functionalized MPS-4, pH 7.0, 22° | Pseudomonas putida | |
1.2.1.46 | 0.518 | - |
formaldehyde | pH 7.0, 22°C, free enzyme | Pseudomonas putida |