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Literature summary extracted from

  • Merzlov, D.A.; Zorov, I.N.; Dotsenko, G.S.; Denisenko, Y.A.; Rozhkova, A.M.; Satrutdinov, A.D.; Rubtsova, E.A.; Kondratieva, E.G.; Sinitsyn, A.P.
    Properties of enzyme preparations and homogeneous enzymes - endoglucanases EG2 Penicillium verruculosum and LAM Myceliophthora thermophila (2015), Biochemistry (Moscow), 80, 473-482 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.4 degradation oligosaccharides with degree of polymerization 2-10 are formed by hydrolysis of beta-glucan. The recombinant enzyme preparations are fast and effective in decreasing the reduced viscosity of wholegrain barley extract than some commercial enzyme preparations Talaromyces verruculosus
3.2.1.6 degradation oligosaccharides with degree of polymerization 2-10 are formed by hydrolysis of beta-glucan and laminarin. The recombinant enzyme preparations are fast and effective in decreasing the reduced viscosity of wholegrain barley extract than some commercial enzyme preparations Thermothelomyces heterothallicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Talaromyces verruculosus Talaromyces verruculosus
3.2.1.6 expression in Talaromyces verruculosus Thermothelomyces heterothallicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 beta-Glucan Km value 22.8 mg/ml, pH 5.0, 50°C Talaromyces verruculosus
3.2.1.6 additional information
-
 Laminarin Km value 1.1 mg/ml, pH 5.0, 50°C Thermothelomyces heterothallicus
3.2.1.6 additional information
-
 beta-Glucan Km value 2.5 mg/ml, pH 5.0, 50°C Thermothelomyces heterothallicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.6 extracellular
-
 Thermothelomyces heterothallicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Talaromyces verruculosus A0A1U7Q1U3
-
-
3.2.1.6 Thermothelomyces heterothallicus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 75
-
 substrate beta-glucan, pH 5.0, 50°C Talaromyces verruculosus
3.2.1.4 76
-
 substrate carboxymethyl cellulose, pH 5.0, 50°C Talaromyces verruculosus
3.2.1.4 94
-
 substrate lichenan, pH 5.0, 50°C Talaromyces verruculosus
3.2.1.6 5.3
-
 substrate curdlan, pH 5.0, 50°C Thermothelomyces heterothallicus
3.2.1.6 15
-
 substrate laminarin, pH 5.0, 50°C Thermothelomyces heterothallicus
3.2.1.6 54
-
 substrate beta-glucan, pH 5.0, 50°C Thermothelomyces heterothallicus
3.2.1.6 56
-
 substrate lichenan, pH 5.0, 50°C Thermothelomyces heterothallicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 beta-glucan + H2O degree of hydrolysis reaches 10%, products are oligosaccharides with degree of polymerization 2-10 Talaromyces verruculosus ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Talaromyces verruculosus ?
-
 ?
3.2.1.4 lichenan + H2O
-
 Talaromyces verruculosus ?
-
 ?
3.2.1.4 additional information no substrates: laminarin, curdlan Talaromyces verruculosus ?
-
 ?
3.2.1.6 beta-glucan + H2O degree of hydrolysis reaches 10%, products are oligosaccharides with degree of polymerization 2-10 Thermothelomyces heterothallicus ?
-
 ?
3.2.1.6 curdlan + H2O
-
 Thermothelomyces heterothallicus ?
-
 ?
3.2.1.6 laminarin + H2O
-
 Thermothelomyces heterothallicus ?
-
 ?
3.2.1.6 lichenan + H2O
-
 Thermothelomyces heterothallicus ?
-
 ?
3.2.1.6 additional information no substrates: xylan, microcrystalline cellulose, and 4-nitrophenylbeta-D-glucopyranoside Thermothelomyces heterothallicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 42000, SDS-PAGE Talaromyces verruculosus
3.2.1.6 ? x * 31000, SDS-PAGE Thermothelomyces heterothallicus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 EG2
-
 Talaromyces verruculosus
3.2.1.6 LAM
-
 Thermothelomyces heterothallicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 61 80
-
 Talaromyces verruculosus
3.2.1.6 59 67
-
 Thermothelomyces heterothallicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 32 85 more than 50% of maximum activity within Talaromyces verruculosus
3.2.1.6 45 70 more than 50% of maximum activity within Thermothelomyces heterothallicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.4 156
-
 beta-Glucan pH 5.0, 50°C Talaromyces verruculosus
3.2.1.6 156
-
 Laminarin pH 5.0, 50°C Thermothelomyces heterothallicus
3.2.1.6 156
-
 beta-Glucan pH 5.0, 50°C Thermothelomyces heterothallicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 3.9 4.9
-
 Talaromyces verruculosus
3.2.1.6 4.3 5.2
-
 Thermothelomyces heterothallicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 3.4 5.6 more than 50% of maximum activity within Talaromyces verruculosus
3.2.1.6 3.8 6 more than 50% of maximum activity within Thermothelomyces heterothallicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 beta-Glucan kcat/Km value 6.6 ml/s/mg, pH 5.0, 50°C Talaromyces verruculosus
3.2.1.6 additional information
-
 Laminarin kcat/Km value 10.6 ml/s/mg, pH 5.0, 50°C Thermothelomyces heterothallicus
3.2.1.6 additional information
-
 beta-Glucan kcat/Km value 19.8 ml/s/mg, pH 5.0, 50°C Thermothelomyces heterothallicus