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Literature summary extracted from

  • Linde, D.; Pogni, R.; Canellas, M.; Lucas, F.; Guallar, V.; Baratto, M.C.; Sinicropi, A.; Saez-Jimenez, V.; Coscolin, C.; Romero, A.; Medrano, F.J.; Ruiz-Duenas, F.J.; Martinez, A.T.
    Catalytic surface radical in dye-decolorizing peroxidase a computational, spectroscopic and site-directed mutagenesis study (2015), Biochem. J., 466, 253-262 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.19 expressed in Escherichia coli BL21(DE3) cells Auricularia auricula-judae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.19 hanging drop vapor diffusion method, using 32.5% (w/v) PEG 4000 as precipitant Auricularia auricula-judae

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.19 G169L the mutant shows strongly increased activity compared to the wild type enzyme Auricularia auricula-judae
1.11.1.19 W377S inactive Auricularia auricula-judae
1.11.1.19 Y147F/Y337F the mutant shows reduced activity compared to the wild type enzyme Auricularia auricula-judae
1.11.1.19 Y147S the mutant shows reduced activity compared to the wild type enzyme Auricularia auricula-judae
1.11.1.19 Y337S the mutant shows about wild type activity Auricularia auricula-judae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.19 0.0106
-
 Reactive Blue 5 mutant enzyme W377S, at pH 3.5 and 25°C Auricularia auricula-judae
1.11.1.19 0.0156
-
 Reactive Blue 5 wild type enzyme, at pH 3.5 and 25°C Auricularia auricula-judae
1.11.1.19 0.09
-
 Reactive Blue 19 wild type enzyme, at pH 3.5 and 25°C Auricularia auricula-judae
1.11.1.19 0.121
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.0 and 25°C Auricularia auricula-judae
1.11.1.19 0.703
-
 2,6-dimethoxyphenol wild type enzyme, at pH 3.0 and 25°C Auricularia auricula-judae
1.11.1.19 2.75
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W377S, at pH 3.0 and 25°C Auricularia auricula-judae
1.11.1.19 7.36
-
 veratryl alcohol mutant enzyme W377S, at pH 2.5 and 25°C Auricularia auricula-judae

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.19 Auricularia auricula-judae I2DBY1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.19
-
 Auricularia auricula-judae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.19 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Auricularia auricula-judae ?
-
 ?
1.11.1.19 2,6-dimethoxyphenol + H2O2 + H+
-
 Auricularia auricula-judae ?
-
 ?
1.11.1.19 Reactive Blue 19 + H2O2 + H+
-
 Auricularia auricula-judae ?
-
 ?
1.11.1.19 Reactive Blue 5 + H2O2 + H+
-
 Auricularia auricula-judae ?
-
 ?
1.11.1.19 veratryl alcohol + H2O2 + H+
-
 Auricularia auricula-judae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.19 dye-decolorizing peroxidase
-
 Auricularia auricula-judae
1.11.1.19 DyP-I
-
 Auricularia auricula-judae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.19 0.23
-
 veratryl alcohol mutant enzyme W377S, at pH 2.5 and 25°C Auricularia auricula-judae
1.11.1.19 0.7
-
 Reactive Blue 5 mutant enzyme W377S, at pH 3.5 and 25°C Auricularia auricula-judae
1.11.1.19 4.8
-
 Reactive Blue 5 wild type enzyme, at pH 3.5 and 25°C Auricularia auricula-judae
1.11.1.19 120
-
 2,6-dimethoxyphenol wild type enzyme, at pH 3.0 and 25°C Auricularia auricula-judae
1.11.1.19 171
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W377S, at pH 3.0 and 25°C Auricularia auricula-judae
1.11.1.19 224
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.0 and 25°C Auricularia auricula-judae
1.11.1.19 224
-
 Reactive Blue 19 wild type enzyme, at pH 3.5 and 25°C Auricularia auricula-judae

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.19 heme
-
 Auricularia auricula-judae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.19 0.032
-
 veratryl alcohol mutant enzyme W377S, at pH 2.5 and 25°C Auricularia auricula-judae
1.11.1.19 0.096
-
 veratryl alcohol wild type enzyme, at pH 2.5 and 25°C Auricularia auricula-judae
1.11.1.19 62
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W377S, at pH 3.0 and 25°C Auricularia auricula-judae
1.11.1.19 68
-
 Reactive Blue 5 mutant enzyme W377S, at pH 3.5 and 25°C Auricularia auricula-judae
1.11.1.19 200
-
 2,6-dimethoxyphenol wild type enzyme, at pH 3.0 and 25°C Auricularia auricula-judae
1.11.1.19 310
-
 Reactive Blue 5 wild type enzyme, at pH 3.5 and 25°C Auricularia auricula-judae
1.11.1.19 1850
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.0 and 25°C Auricularia auricula-judae
1.11.1.19 2460
-
 Reactive Blue 19 wild type enzyme, at pH 3.5 and 25°C Auricularia auricula-judae