Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nishigaya, Y.; Fujimoto, Z.; Yamazaki, T.
    Optimized inhibition assays reveal different inhibitory responses of hydroxylamine oxidoreductases from beta- and gamma-proteobacterial ammonium-oxidizing bacteria (2016), Biochem. Biophys. Res. Commun., 476, 127-133 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.2.6 additional information no significant changes in the NeHAO and NoHAO activities by addition of 0.5% DMSO and 0.01% Triton X-100 Nitrosospira multiformis
1.7.2.6 additional information no significant changes in the NeHAO and NoHAO activities by addition of 0.5% DMSO and 0.01% Triton X-100 Nitrosococcus oceani
1.7.2.6 additional information no significant changes in the NeHAO and NoHAO activities by addition of 0.5% DMSO and 0.01% Triton X-100 Nitrosomonas europaea
1.7.2.6 additional information no significant changes in the NeHAO and NoHAO activities by addition of 0.5% DMSO and 0.01% Triton X-100 Nitrosomonas sp. JPCCT2

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.2.6 gene hao, sequence comparisons Nitrosospira multiformis
1.7.2.6 gene hao, sequence comparisons Nitrosococcus oceani
1.7.2.6 gene hao, sequence comparisons Nitrosomonas europaea
1.7.2.6 gene hao, sequence comparisons Nitrosomonas sp. JPCCT2

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.7.2.6 purified enzyme, 2 weeks Nitrosomonas europaea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.6 additional information no significant changes in the NeHAO and NoHAO activities by addition of 0.5% DMSO and 0.01% Triton X-100 Nitrosococcus oceani
1.7.2.6 additional information no significant changes in the NeHAO and NoHAO activities by addition of 0.5% DMSO and 0.01% Triton X-100 Nitrosomonas europaea
1.7.2.6 additional information no significant changes in the NeHAO and NoHAO activities by addition of 0.5% DMSO and 0.01% Triton X-100 Nitrosomonas sp. JPCCT2
1.7.2.6 additional information no significant changes in the NeHAO and NoHAO activities by addition of 0.5% DMSO and 0.01% Triton X-100 Nitrosospira multiformis
1.7.2.6 phenylhydrazine an HAO enzyme specific inhibitor, slight inhibition of enzyme NoHAO activity at 0.01 mM Nitrosococcus oceani
1.7.2.6 phenylhydrazine an HAO enzyme specific inhibitor, inhibits about 60% of enzyme NeHAO activity at 0.01 mM Nitrosomonas europaea
1.7.2.6 phenylhydrazine an HAO enzyme specific inhibitor Nitrosomonas sp. JPCCT2
1.7.2.6 phenylhydrazine an HAO enzyme specific inhibitor Nitrosospira multiformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.6 Fe2+ heme Nitrosospira multiformis
1.7.2.6 Fe2+ heme Nitrosococcus oceani
1.7.2.6 Fe2+ heme Nitrosomonas europaea
1.7.2.6 Fe2+ heme Nitrosomonas sp. JPCCT2

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.6 hydroxylamine + H2O + 4 ferricytochrome c Nitrosospira multiformis
-
nitrite + 4 ferrocytochrome c + 5 H+
-
?
1.7.2.6 hydroxylamine + H2O + 4 ferricytochrome c Nitrosococcus oceani
-
nitrite + 4 ferrocytochrome c + 5 H+
-
?
1.7.2.6 hydroxylamine + H2O + 4 ferricytochrome c Nitrosomonas europaea
-
nitrite + 4 ferrocytochrome c + 5 H+
-
?
1.7.2.6 hydroxylamine + H2O + 4 ferricytochrome c Nitrosomonas sp. JPCCT2
-
nitrite + 4 ferrocytochrome c + 5 H+
-
?
1.7.2.6 hydroxylamine + H2O + 4 ferricytochrome c Nitrosomonas europaea ATCC 19718
-
nitrite + 4 ferrocytochrome c + 5 H+
-
?
1.7.2.6 hydroxylamine + H2O + 4 ferricytochrome c Nitrosococcus oceani ATCC 19707
-
nitrite + 4 ferrocytochrome c + 5 H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.6 Nitrosococcus oceani
-
-
-
1.7.2.6 Nitrosococcus oceani ATCC 19707
-
-
-
1.7.2.6 Nitrosomonas europaea Q50925
-
-
1.7.2.6 Nitrosomonas europaea ATCC 19718 Q50925
-
-
1.7.2.6 Nitrosomonas sp. JPCCT2
-
-
-
1.7.2.6 Nitrosospira multiformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.6 native enzyme from strain ATCC 19718 by anion exchange chromatography and gel filtration Nitrosococcus oceani
1.7.2.6 native enzyme from strain ATCC 19718 by anion exchange chromatography, gel filtration, and hdroxyapatite chromatography Nitrosomonas europaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.6 hydroxylamine + H2O + 4 ferricytochrome c
-
Nitrosospira multiformis nitrite + 4 ferrocytochrome c + 5 H+
-
?
1.7.2.6 hydroxylamine + H2O + 4 ferricytochrome c
-
Nitrosococcus oceani nitrite + 4 ferrocytochrome c + 5 H+
-
?
1.7.2.6 hydroxylamine + H2O + 4 ferricytochrome c
-
Nitrosomonas europaea nitrite + 4 ferrocytochrome c + 5 H+
-
?
1.7.2.6 hydroxylamine + H2O + 4 ferricytochrome c
-
Nitrosomonas sp. JPCCT2 nitrite + 4 ferrocytochrome c + 5 H+
-
?
1.7.2.6 hydroxylamine + H2O + 4 ferricytochrome c usage of horse heart cytochrome c Nitrosospira multiformis nitrite + 4 ferrocytochrome c + 5 H+
-
?
1.7.2.6 hydroxylamine + H2O + 4 ferricytochrome c usage of horse heart cytochrome c Nitrosococcus oceani nitrite + 4 ferrocytochrome c + 5 H+
-
?
1.7.2.6 hydroxylamine + H2O + 4 ferricytochrome c usage of horse heart cytochrome c Nitrosomonas europaea nitrite + 4 ferrocytochrome c + 5 H+
-
?
1.7.2.6 hydroxylamine + H2O + 4 ferricytochrome c usage of horse heart cytochrome c Nitrosomonas sp. JPCCT2 nitrite + 4 ferrocytochrome c + 5 H+
-
?
1.7.2.6 hydroxylamine + H2O + 4 ferricytochrome c
-
Nitrosomonas europaea ATCC 19718 nitrite + 4 ferrocytochrome c + 5 H+
-
?
1.7.2.6 hydroxylamine + H2O + 4 ferricytochrome c usage of horse heart cytochrome c Nitrosomonas europaea ATCC 19718 nitrite + 4 ferrocytochrome c + 5 H+
-
?
1.7.2.6 hydroxylamine + H2O + 4 ferricytochrome c
-
Nitrosococcus oceani ATCC 19707 nitrite + 4 ferrocytochrome c + 5 H+
-
?
1.7.2.6 hydroxylamine + H2O + 4 ferricytochrome c usage of horse heart cytochrome c Nitrosococcus oceani ATCC 19707 nitrite + 4 ferrocytochrome c + 5 H+
-
?
1.7.2.6 additional information optimization of HAO activity assay method, evaluation, overview Nitrosospira multiformis ?
-
?
1.7.2.6 additional information optimization of HAO activity assay method, evaluation, overview Nitrosococcus oceani ?
-
?
1.7.2.6 additional information optimization of HAO activity assay method, evaluation, overview Nitrosomonas europaea ?
-
?
1.7.2.6 additional information optimization of HAO activity assay method, evaluation, overview Nitrosomonas sp. JPCCT2 ?
-
?
1.7.2.6 additional information optimization of HAO activity assay method, evaluation, overview Nitrosomonas europaea ATCC 19718 ?
-
?
1.7.2.6 additional information optimization of HAO activity assay method, evaluation, overview Nitrosococcus oceani ATCC 19707 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.7.2.6 HAO
-
Nitrosospira multiformis
1.7.2.6 HAO
-
Nitrosococcus oceani
1.7.2.6 HAO
-
Nitrosomonas europaea
1.7.2.6 HAO
-
Nitrosomonas sp. JPCCT2
1.7.2.6 hydroxylamine oxidoreductase
-
Nitrosospira multiformis
1.7.2.6 hydroxylamine oxidoreductase
-
Nitrosococcus oceani
1.7.2.6 hydroxylamine oxidoreductase
-
Nitrosomonas europaea
1.7.2.6 hydroxylamine oxidoreductase
-
Nitrosomonas sp. JPCCT2
1.7.2.6 NeHAO
-
Nitrosomonas europaea
1.7.2.6 NoHAO
-
Nitrosococcus oceani

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.2.6 25
-
assay at Nitrosospira multiformis
1.7.2.6 25
-
assay at Nitrosococcus oceani
1.7.2.6 25
-
assay at Nitrosomonas europaea
1.7.2.6 25
-
assay at Nitrosomonas sp. JPCCT2

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.6 7
-
assay at Nitrosospira multiformis
1.7.2.6 7
-
assay at Nitrosomonas sp. JPCCT2
1.7.2.6 9.4
-
-
Nitrosomonas europaea
1.7.2.6 10
-
-
Nitrosococcus oceani

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.6 cytochrome c with heme Nitrosospira multiformis
1.7.2.6 cytochrome c with heme Nitrosococcus oceani
1.7.2.6 cytochrome c with heme Nitrosomonas europaea
1.7.2.6 cytochrome c with heme Nitrosomonas sp. JPCCT2
1.7.2.6 heme c-type hemes and P-460-type heme Nitrosospira multiformis
1.7.2.6 heme c-type hemes and P-460-type heme Nitrosococcus oceani
1.7.2.6 heme c-type hemes and P-460-type heme Nitrosomonas europaea
1.7.2.6 heme c-type hemes and P-460-type heme Nitrosomonas sp. JPCCT2

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.7.2.6 0.00092
-
pH 7.0, 25°C Nitrosomonas sp. JPCCT2 phenylhydrazine
1.7.2.6 0.00101
-
pH 7.0, 25°C Nitrosomonas europaea phenylhydrazine
1.7.2.6 0.00123
-
pH 7.0, 25°C Nitrosospira multiformis phenylhydrazine
1.7.2.6 0.00289
-
pH 7.0, 25°C Nitrosococcus oceani phenylhydrazine