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Literature summary extracted from

  • Dotsenko, G.; Tong, X.; Pilgaard, B.; Busk, P.; Lange, L.
    Acidic-alkaline ferulic acid esterase from Chaetomium thermophilum var. dissitum Molecular cloning and characterization of recombinant enzyme expressed in Pichia pastoris (2016), Biocatal. Agricult. Biotechnol., 5, 48-55 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 expression in Pichia pastoris KM71H. Thermochaetoides dissita

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.016
-
 methyl p-coumarate pH 6.0, 55°C Thermochaetoides dissita
3.1.1.73 0.025
-
 ethyl ferulate pH 6.0, 55°C Thermochaetoides dissita
3.1.1.73 0.031
-
 methyl caffeate pH 6.0, 55°C Thermochaetoides dissita
3.1.1.73 0.25
-
 1-naphthyl acetate pH 6.0, 55°C Thermochaetoides dissita
3.1.1.73 0.54
-
 4-nitrophenyl butanoate pH 6.0, 55°C Thermochaetoides dissita
3.1.1.73 0.7
-
 4-nitrophenyl acetate pH 6.0, 55°C Thermochaetoides dissita
3.1.1.73 0.8
-
 4-nitrophenyl ferulate pH 6.0, 55°C Thermochaetoides dissita

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Thermochaetoides dissita A0A1B1LML0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 1-naphthyl acetate + H2O
-
 Thermochaetoides dissita 1-naphthol + acetate
-
 ?
3.1.1.73 4-nitrophenyl acetate + H2O
-
 Thermochaetoides dissita 4-nitrophenol + acetate
-
 ?
3.1.1.73 4-nitrophenyl butanoate + H2O
-
 Thermochaetoides dissita 4-nitrophenol + butanoate
-
 ?
3.1.1.73 4-nitrophenyl ferulate + H2O
-
 Thermochaetoides dissita 4-nitrophenol + ferulate
-
 ?
3.1.1.73 arabinoxylan + H2O
-
 Thermochaetoides dissita ferulic acid + ?
-
 ?
3.1.1.73 ethyl ferulate + H2O
-
 Thermochaetoides dissita ethanol + ferulate
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Thermochaetoides dissita methanol + caffeate
-
 ?
3.1.1.73 methyl p-coumarate + H2O
-
 Thermochaetoides dissita methanol + p-coumarate
-
 ?
3.1.1.73 additional information Fae is active towards synthetic esters of ferulic, p-coumaric, and caffeic acids, as well as towards wide range of 4-nitrophenyl substrates Thermochaetoides dissita ?
-
 ?
3.1.1.73 rye grass + H2O
-
 Thermochaetoides dissita ferulic acid + p-coumaric acid + ?
-
 ?
3.1.1.73 wheat straw + H2O
-
 Thermochaetoides dissita ferulic acid + p-coumaric acid + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 ? x * 29600, calculated, x * 30000, SDS-PAGE Thermochaetoides dissita

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FAE
-
 Thermochaetoides dissita
3.1.1.73 ferulic acid esterase
-
 Thermochaetoides dissita

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 55
-
-
 Thermochaetoides dissita

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.73 45 65 more than 70% of the maximal activity Thermochaetoides dissita

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 40
-
 24 h, 99% residual activity Thermochaetoides dissita
3.1.1.73 50
-
 24 h, 98% residual activity Thermochaetoides dissita
3.1.1.73 60
-
 24 h, 73% residual activity Thermochaetoides dissita

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 3.1
-
 methyl caffeate pH 6.0, 55°C Thermochaetoides dissita
3.1.1.73 4.1
-
 ethyl ferulate pH 6.0, 55°C Thermochaetoides dissita
3.1.1.73 5.8
-
 methyl p-coumarate pH 6.0, 55°C Thermochaetoides dissita
3.1.1.73 24
-
 4-nitrophenyl ferulate pH 6.0, 55°C Thermochaetoides dissita
3.1.1.73 32
-
 1-naphthyl acetate pH 6.0, 55°C Thermochaetoides dissita
3.1.1.73 59
-
 4-nitrophenyl butanoate pH 6.0, 55°C Thermochaetoides dissita
3.1.1.73 60
-
 4-nitrophenyl acetate pH 6.0, 55°C Thermochaetoides dissita

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 6
-
-
 Thermochaetoides dissita

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 5 8 more than 80% of maximal activity Thermochaetoides dissita

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.73 4 10 4°C, 24 h, 70% residual activity Thermochaetoides dissita

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.73 Thermochaetoides dissita calculated
-
 6.8

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.73 30
-
 4-nitrophenyl ferulate pH 6.0, 55°C Thermochaetoides dissita
3.1.1.73 86
-
 4-nitrophenyl acetate pH 6.0, 55°C Thermochaetoides dissita
3.1.1.73 99
-
 methyl caffeate pH 6.0, 55°C Thermochaetoides dissita
3.1.1.73 109
-
 4-nitrophenyl butanoate pH 6.0, 55°C Thermochaetoides dissita
3.1.1.73 128
-
 1-naphthyl acetate pH 6.0, 55°C Thermochaetoides dissita
3.1.1.73 164
-
 ethyl ferulate pH 6.0, 55°C Thermochaetoides dissita
3.1.1.73 363
-
 methyl p-coumarate pH 6.0, 55°C Thermochaetoides dissita