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Literature summary extracted from
- Purification and characterization of cellobiose dehydrogenase, a novel extracellular hemoflavoenzyme from the white-rot fungus Phanerochaete chrysosporium (1993), Arch. Biochem. Biophys., 300, 705-713 .
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.18 | 0.0012 | - |
ferricytochrome c | pH 4.5, 22°C | Phanerodontia chrysosporium |  |
| 1.1.99.18 | 0.025 | - |
cellobiose | pH 4.5, 22°C | Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.055 | - |
cellopentaose | pH 4.5, 22°C | Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.061 | - |
cellotetraose | pH 4.5, 22°C | Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.062 | - |
cellotriose | pH 4.5, 22°C | Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.63 | - |
lactose | pH 4.5, 22°C | Phanerodontia chrysosporium | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.18 | Iron | the ferric enzyme does not bind azide or cyanide, implying that the heme iron is probably hexacoordinate | Phanerodontia chrysosporium | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 90000 | - |
gel filtration | Phanerodontia chrysosporium |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.18 | Phanerodontia chrysosporium | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 1.1.99.18 | glycoprotein | carbohydrate content 9.4% | Phanerodontia chrysosporium |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.99.18 | - |
Phanerodontia chrysosporium |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 10.3 | - |
pH 4.5, 22°C | Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.18 | cellobiose + ferricytochrome c | cellobiose is the preferred substrate and cytochrome c is the preferred electron acceptor | Phanerodontia chrysosporium | cellobiono-1,5-lactone + ferrocytochrome c | - |
? | |
| 1.1.99.18 | cellopentaose + ferricytochrome c | - |
Phanerodontia chrysosporium | cellopentaono-1,5-lactone + ferrocytochrome c | - |
? | |
| 1.1.99.18 | cellotetraose + ferricytochrome c | - |
Phanerodontia chrysosporium | cellotetraono-1,5-lactone + ferrocytochrome c | - |
? | |
| 1.1.99.18 | cellotriose + ferricytoferricytochrome c chrome c | - |
Phanerodontia chrysosporium | cellotriono-1,5-lactone + ferrocytochrome c | - |
? | |
| 1.1.99.18 | lactose + ferricytochrome c | - |
Phanerodontia chrysosporium | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + ferrocytochrome c | - |
? | |
| 1.1.99.18 | additional information | electron acceptors are cytochrome c, benzoquinones, dichlorophenolindophenol and Mn3+. In the absence of these electron acceptors, oxygen serves as a poor electron acceptor and is reduced to H2O2 | Phanerodontia chrysosporium | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.99.18 | monomer | 1 * 90000, SDS-PAGE | Phanerodontia chrysosporium |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.99.18 | Cdh | - |
Phanerodontia chrysosporium |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 50 | - |
stable up to | Phanerodontia chrysosporium |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.18 | 20.5 | - |
ferricytochrome c | pH 4.5, 22°C | Phanerodontia chrysosporium | |
| 1.1.99.18 | 20.7 | - |
cellotetraose | pH 4.5, 22°C | Phanerodontia chrysosporium | |
| 1.1.99.18 | 21.5 | - |
cellopentaose | pH 4.5, 22°C | Phanerodontia chrysosporium | |
| 1.1.99.18 | 24 | - |
cellobiose | pH 4.5, 22°C | Phanerodontia chrysosporium | |
| 1.1.99.18 | 24 | - |
cellotriose | pH 4.5, 22°C | Phanerodontia chrysosporium | |
| 1.1.99.18 | 28.8 | - |
lactose | pH 4.5, 22°C | Phanerodontia chrysosporium | |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 3 | 10 | - |
Phanerodontia chrysosporium |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.18 | cytochrome b | one heme b and one FAD per monomer. The native ferric form of the enzyme has absorption maxima at 420, 529, and 570 nm. The ferric enzyme does not bind azide or cyanide, implying that the heme iron is probably hexacoordinate | Phanerodontia chrysosporium | |
| 1.1.99.18 | FAD | one heme b and one FAD per monomer | Phanerodontia chrysosporium | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.18 | 46 | - |
lactose | pH 4.5, 22°C | Phanerodontia chrysosporium | |
| 1.1.99.18 | 339 | - |
cellotetraose | pH 4.5, 22°C | Phanerodontia chrysosporium | |
| 1.1.99.18 | 387 | - |
cellotriose | pH 4.5, 22°C | Phanerodontia chrysosporium | |
| 1.1.99.18 | 391 | - |
cellopentaose | pH 4.5, 22°C | Phanerodontia chrysosporium | |
| 1.1.99.18 | 960 | - |
cellobiose | pH 4.5, 22°C | Phanerodontia chrysosporium | |
| 1.1.99.18 | 17083 | - |
ferricytochrome c | pH 4.5, 22°C | Phanerodontia chrysosporium | |
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