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Literature summary extracted from

  • Tuisel, H.; Sinclair, R.; Bumpus, J.A.; Ashbaugh, W.; Brock, B.J.; Aust, S.D.
    Lignin peroxidase H2 from Phanerochaete chrysosporium purification, characterization and stability to temperature and pH (1990), Arch. Biochem. Biophys., 279, 158-166 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.14 3-Amino-1,2,4-triazole
-
 Phanerodontia chrysosporium
1.11.1.14 azide
-
 Phanerodontia chrysosporium
1.11.1.14 cyanide
-
 Phanerodontia chrysosporium
1.11.1.14 EDTA
-
 Phanerodontia chrysosporium
1.11.1.14 H2O2 incubation of lignin peroxidase H2 with excess H2O2 in the absence of another reducing substrate results in partial and irreversible inactivation of the enzyme Phanerodontia chrysosporium
1.11.1.14 additional information not inhibitory: chloride Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.14 0.009
-
 H2O2 pH 4.5, 25°C Phanerodontia chrysosporium
1.11.1.14 0.047
-
 H2O2 pH 3.5, 25°C Phanerodontia chrysosporium
1.11.1.14 0.091
-
 H2O2 pH 2.5, 25°C Phanerodontia chrysosporium
1.11.1.14 0.132
-
 veratryl alcohol pH 4.5, 25°C Phanerodontia chrysosporium
1.11.1.14 0.167
-
 veratryl alcohol pH 3.5, 25°C Phanerodontia chrysosporium
1.11.1.14 0.21
-
 veratryl alcohol pH 2.5, 25°C Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.14 Phanerodontia chrysosporium P11542
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.14 veratryl alcohol + H2O2
-
 Phanerodontia chrysosporium veratraldehyde + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.14 ? x * 38500, SDS-PAGE Phanerodontia chrysosporium

Synonyms

EC Number Synonyms Comment Organism
1.11.1.14 Glg4
-
 Phanerodontia chrysosporium
1.11.1.14 ligninase H2
-
 Phanerodontia chrysosporium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.14 25
-
 substrate veratryl alcohol, pH 2.5 Phanerodontia chrysosporium
1.11.1.14 45
-
 substrate veratryl alcohol, pH 3.5 Phanerodontia chrysosporium
1.11.1.14 45 60 substrate veratryl alcohol, pH 4.5 Phanerodontia chrysosporium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.14 40
-
 21 h, pH 4.0-6.5, 80% residual activity Phanerodontia chrysosporium
1.11.1.14 50
-
 stable for 48 h up to 50°C Phanerodontia chrysosporium
1.11.1.14 60
-
 6 h, complete loss of activity Phanerodontia chrysosporium
1.11.1.14 70
-
 min, complete loss of activity Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.14 2.5
-
 substrate veratryl alcohol, 25°C Phanerodontia chrysosporium
1.11.1.14 3
-
 substrate veratryl alcohol, 35°C Phanerodontia chrysosporium
1.11.1.14 3.5
-
 substrate veratryl alcohol, 45°C Phanerodontia chrysosporium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.14 4 7.5
-
 Phanerodontia chrysosporium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.14 Phanerodontia chrysosporium isoelectric focusing
-
 4.3