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Literature summary extracted from

  • Kasai, K.; Hashiguchi, K.; Takahashi, H.; Kasai, A.; Takeda, S.; Nakano, M.; Ishikawa, T.; Nakamura, T.; Miura, T.
    Recombinant production and evaluation of an antibacterial L-amino acid oxidase derived from flounder Platichthys stellatus (2015), Appl. Microbiol. Biotechnol., 99, 6693-6703 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.2 expressed in Pichia pastoris strain GS115 Platichthys stellatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.2 DL-norleucine + H2O + O2 Platichthys stellatus
-
2-oxocaproate + NH3 + H2O2
-
?
1.4.3.2 L-Arg + H2O + O2 Platichthys stellatus
-
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
?
1.4.3.2 L-Asp + H2O + O2 Platichthys stellatus
-
oxaloacetate + NH3 + H2O2
-
?
1.4.3.2 L-Glu + H2O + O2 Platichthys stellatus
-
2-oxoglutarate + NH3 + H2O2
-
?
1.4.3.2 L-His + H2O + O2 Platichthys stellatus
-
2-oxo-4-imidazolepropionic acid + NH3 + H2O2
-
?
1.4.3.2 L-Ile + H2O + O2 Platichthys stellatus
-
3-methyl-2-oxo-pentanoic acid + NH3 + H2O2
-
?
1.4.3.2 L-Leu + H2O + O2 Platichthys stellatus
-
2-oxo-4-methylvaleric acid + NH3 + H2O2
-
?
1.4.3.2 L-Lys + H2O + O2 Platichthys stellatus
-
6-amino-2-oxo-hexanoic acid + NH3 + H2O2
-
?
1.4.3.2 L-Met + H2O + O2 Platichthys stellatus
-
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
?
1.4.3.2 L-Orn + H2O + O2 Platichthys stellatus
-
2-oxo-5-aminopentanoate + NH3 + H2O2
-
?
1.4.3.2 L-Phe + H2O + O2 Platichthys stellatus
-
phenylpyruvate + NH3 + H2O2
-
?
1.4.3.2 L-Trp + H2O + O2 Platichthys stellatus
-
indole-3-pyruvate + NH3 + H2O2
-
?
1.4.3.2 L-Tyr + H2O + O2 Platichthys stellatus
-
4-hydroxyphenylpyruvate + NH3 + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Platichthys stellatus D2KWB6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2 HiPrep phenyl column chromatography and Ni-NTA agarose column chromatography Platichthys stellatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 DL-norleucine + H2O + O2
-
Platichthys stellatus 2-oxocaproate + NH3 + H2O2
-
?
1.4.3.2 L-Arg + H2O + O2
-
Platichthys stellatus 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
?
1.4.3.2 L-Asp + H2O + O2
-
Platichthys stellatus oxaloacetate + NH3 + H2O2
-
?
1.4.3.2 L-Glu + H2O + O2
-
Platichthys stellatus 2-oxoglutarate + NH3 + H2O2
-
?
1.4.3.2 L-His + H2O + O2
-
Platichthys stellatus 2-oxo-4-imidazolepropionic acid + NH3 + H2O2
-
?
1.4.3.2 L-Ile + H2O + O2
-
Platichthys stellatus 3-methyl-2-oxo-pentanoic acid + NH3 + H2O2
-
?
1.4.3.2 L-Leu + H2O + O2
-
Platichthys stellatus 2-oxo-4-methylvaleric acid + NH3 + H2O2
-
?
1.4.3.2 L-Lys + H2O + O2
-
Platichthys stellatus 6-amino-2-oxo-hexanoic acid + NH3 + H2O2
-
?
1.4.3.2 L-Met + H2O + O2
-
Platichthys stellatus 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
?
1.4.3.2 L-Orn + H2O + O2
-
Platichthys stellatus 2-oxo-5-aminopentanoate + NH3 + H2O2
-
?
1.4.3.2 L-Phe + H2O + O2
-
Platichthys stellatus phenylpyruvate + NH3 + H2O2
-
?
1.4.3.2 L-Trp + H2O + O2
-
Platichthys stellatus indole-3-pyruvate + NH3 + H2O2
-
?
1.4.3.2 L-Tyr + H2O + O2
-
Platichthys stellatus 4-hydroxyphenylpyruvate + NH3 + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.4.3.2 ? x * 52000, SDS-PAGE Platichthys stellatus

Synonyms

EC Number Synonyms Comment Organism
1.4.3.2 LAAO1
-
Platichthys stellatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.2 FAD
-
Platichthys stellatus

General Information

EC Number General Information Comment Organism
1.4.3.2 physiological function the enzyme inhibits the growth of bacteria (Staphylococci, Yersinia sp. Staphylococcus aureus, and Staphylococcus epidermidis) present in mucus Platichthys stellatus