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Literature summary extracted from

  • Rocaboy-Faquet, E.; Noguer, T.; Romdhane, S.; Bertrand, C.; Dayan, F.E.; Barthelmebs, L.
    Novel bacterial bioassay for a high-throughput screening of 4-hydroxyphenylpyruvate dioxygenase inhibitors (2014), Appl. Microbiol. Biotechnol., 98, 7243-7252 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.13.11.27 agriculture plant 4-hydroxyphenylpyruvate dioxygenase (HPPD) is the molecular target of a range of commercial synthetic beta-triketone herbicides Their mode of action is based on an irreversible inhibition of HPPD Arabidopsis thaliana
1.13.11.27 drug development plant 4-hydroxyphenylpyruvate dioxygenase (HPPD) is the molecular target for development of specific inhibitors Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.27 gene HPD, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha, determination of protein expression patterns Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.27 leptospermone
-
 Arabidopsis thaliana
1.13.11.27 mesotrione
-
 Arabidopsis thaliana
1.13.11.27 additional information high-throughput screening of 4-hydroxyphenylpyruvate dioxygenase inhibitors Arabidopsis thaliana
1.13.11.27 sulcotrione
-
 Arabidopsis thaliana
1.13.11.27 tembotrione
-
 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.27 4-hydroxyphenylpyruvate + O2 Arabidopsis thaliana
-
 homogentisate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.27 Arabidopsis thaliana P93836
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.27 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.27 4-hydroxyphenylpyruvate + O2
-
 Arabidopsis thaliana homogentisate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.27 ? x * 48000, recombinant His-tagged enzyme, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.13.11.27 HPD
-
 Arabidopsis thaliana
1.13.11.27 HPPD
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.27 37
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.27 7.6
-
 assay at Arabidopsis thaliana