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Literature summary extracted from

  • Voutilainen, S.P.; Boer, H.; Alapuranen, M.; Jaenis, J.; Vehmaanperae, J.; Koivula, A.
    Improving the thermostability and activity of Melanocarpus albomyces cellobiohydrolase Cel7B (2009), Appl. Microbiol. Biotechnol., 83, 261-272 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.176 expression in Saccharomyces cerevisiae Melanocarpus albomyces

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.176 G4C/M70C introduction of disulfide bridge, improvement of thermostability by 2.5 degrees and increased activity towards microcrystalline cellulose at 75°C Melanocarpus albomyces
3.2.1.176 G4C/M70C/S290T improvement of thermostability by 4.0 degrees and increased activity towards microcrystalline cellulose at 75°C Melanocarpus albomyces
3.2.1.176 additional information linking of a cellulose-binding module improves the thermostability by 2.5 degrees and causes a clear increase in the hydrolysis activity towards Avicel at 70°C Melanocarpus albomyces
3.2.1.176 S290T improvement of thermostability by 1.5 degrees Melanocarpus albomyces

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.176 cellobiose mixed type inhibition Melanocarpus albomyces

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.176 0.23
-
 4-methylumbelliferyl beta-D-lactopyranoside wild-type, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.28
-
 4-methylumbelliferyl beta-D-lactopyranoside mutant S290T, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.3
-
 4-methylumbelliferyl beta-D-lactopyranoside mutant G4C/M70C, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.3
-
 4-methylumbelliferyl beta-D-lactopyranoside mutant G4C/M70C/S290T, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.62
-
 2-chloro-4-nitrophenyl beta-D-lactoside wild-type, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.75
-
 2-chloro-4-nitrophenyl beta-D-lactoside mutant G4C/M70C/S290T, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 1
-
 2-chloro-4-nitrophenyl beta-D-lactoside mutant G4C/M70C, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 1.16
-
 2-chloro-4-nitrophenyl beta-D-lactoside mutant S290T, pH 6.0, 22°C Melanocarpus albomyces

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Melanocarpus albomyces Q8J0K6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 2-chloro-4-nitrophenyl beta-D-lactoside + H2O
-
 Melanocarpus albomyces 2-chloro-4-nitrophenol + D-lactose
-
 ?
3.2.1.176 4-methylumbelliferyl beta-D-lactopyranoside + H2O
-
 Melanocarpus albomyces 4-methylumbelliferone + lactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.176 ? x * 47500, calculated, x * 50000, SDS-PAGE, x * 65000, SDS-PAGE of recombinant protein carrying a cellulose-binding module Melanocarpus albomyces

Synonyms

EC Number Synonyms Comment Organism
3.2.1.176 Cel7B
-
 Melanocarpus albomyces

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.176 68
-
 melting temperature, wild-type Melanocarpus albomyces
3.2.1.176 70
-
 melting temperature, mutant S290T Melanocarpus albomyces
3.2.1.176 70.5
-
 melting temperature, mutant carrying a cellulose-binding module Melanocarpus albomyces
3.2.1.176 71
-
 melting temperature, mutant G4C/M70C Melanocarpus albomyces
3.2.1.176 72.5
-
 melting temperature, mutant G4C/M70C/S290T Melanocarpus albomyces

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.176 0.017
-
 2-chloro-4-nitrophenyl beta-D-lactoside wild-type, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.02
-
 2-chloro-4-nitrophenyl beta-D-lactoside mutant S290T, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.08
-
 4-methylumbelliferyl beta-D-lactopyranoside mutant G4C/M70C, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.11
-
 4-methylumbelliferyl beta-D-lactopyranoside mutant S290T, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.12
-
 4-methylumbelliferyl beta-D-lactopyranoside mutant G4C/M70C/S290T, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.14
-
 4-methylumbelliferyl beta-D-lactopyranoside wild-type, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.165
-
 2-chloro-4-nitrophenyl beta-D-lactoside mutant G4C/M70C/S290T, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.182
-
 2-chloro-4-nitrophenyl beta-D-lactoside mutant G4C/M70C, pH 6.0, 22°C Melanocarpus albomyces

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.176 0.005
-
 cellobiose mutant G4C/M70C/S290T, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.0055
-
 cellobiose mutant G4C/M70C, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.006
-
 cellobiose wild-type, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.0066
-
 cellobiose mutant S290T, pH 6.0, 22°C Melanocarpus albomyces

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.176 0.017
-
 2-chloro-4-nitrophenyl beta-D-lactoside mutant G4C/M70C, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.017
-
 2-chloro-4-nitrophenyl beta-D-lactoside mutant S290T, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.022
-
 2-chloro-4-nitrophenyl beta-D-lactoside mutant G4C/M70C/S290T, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.027
-
 2-chloro-4-nitrophenyl beta-D-lactoside wild-type, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.27
-
 4-methylumbelliferyl beta-D-lactopyranoside mutant G4C/M70C, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.38
-
 4-methylumbelliferyl beta-D-lactopyranoside mutant S290T, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.4
-
 4-methylumbelliferyl beta-D-lactopyranoside mutant G4C/M70C/S290T, pH 6.0, 22°C Melanocarpus albomyces
3.2.1.176 0.62
-
 4-methylumbelliferyl beta-D-lactopyranoside wild-type, pH 6.0, 22°C Melanocarpus albomyces