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Literature summary extracted from

  • Hahn, K.; Neumeister, K.; Mix, A.; Kottke, T.; Groeger, H.; Fischer von Mollard, G.
    Recombinant expression and characterization of a L-amino acid oxidase from the fungus Rhizoctonia solani (2017), Appl. Microbiol. Biotechnol., 101, 2853-2864 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.3.2 SDS enzymatic activity is stimulated 50-100fold by SDS treatment Rhizoctonia solani

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.2 expressed in Escherichia coli BL21 CodonPlus (DE)-RP and Arctic Express (DE) cells Rhizoctonia solani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.2 0.4
-
 L-Arg at pH 8.5 and 30°C Rhizoctonia solani
1.4.3.2 1.3
-
 L-Lys at pH 8.5 and 30°C Rhizoctonia solani
1.4.3.2 5.6
-
 L-Orn at pH 8.5 and 30°C Rhizoctonia solani
1.4.3.2 6.1
-
 L-Leu at pH 8.5 and 30°C Rhizoctonia solani
1.4.3.2 6.8
-
 L-Phe at pH 8.5 and 30°C Rhizoctonia solani
1.4.3.2 11.7
-
 L-phenylalanine methyl ester at pH 8.5 and 30°C Rhizoctonia solani
1.4.3.2 20
-
 L-Met at pH 8.5 and 30°C Rhizoctonia solani

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Rhizoctonia solani
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2 amylose column chromatography and Ni-NTA column chromatography Rhizoctonia solani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 DL-norleucine + H2O + O2 53% activity compared to L-Arg Rhizoctonia solani 2-oxocaproate + NH3 + H2O2
-
 ?
1.4.3.2 L-Arg + H2O + O2 100% activity Rhizoctonia solani 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Asp + H2O + O2 1.0% activity compared to L-Arg Rhizoctonia solani oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.2 L-Glu + H2O + O2 1.6% activity compared to L-Arg Rhizoctonia solani 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.2 L-His + H2O + O2 5.2% activity compared to L-Arg Rhizoctonia solani 2-oxo-4-imidazolepropionic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Ile + H2O + O2 3.1% activity compared to L-Arg Rhizoctonia solani 3-methyl-2-oxo-pentanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Leu + H2O + O2 70% activity compared to L-Arg Rhizoctonia solani 2-oxo-4-methylvaleric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-leucine methyl ester + H2O + O2 61% activity compared to L-Arg Rhizoctonia solani methyl 2-oxo-4-methylpentanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Lys + H2O + O2 55% activity compared to L-Arg Rhizoctonia solani 6-amino-2-oxo-hexanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Met + H2O + O2 23% activity compared to L-Arg Rhizoctonia solani 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-methionine methyl ester + H2O + O2 12% activity compared to L-Arg Rhizoctonia solani methyl 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Orn + H2O + O2 60% activity compared to L-Arg Rhizoctonia solani 2-oxo-5-aminopentanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Phe + H2O + O2 88% activity compared to L-Arg Rhizoctonia solani phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-phenylalanine methyl ester + H2O + O2 67% activity compared to L-Arg Rhizoctonia solani methyl phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-Trp + H2O + O2 6.4% activity compared to L-Arg Rhizoctonia solani indole-3-pyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-Tyr + H2O + O2 4.0% activity compared to L-Arg Rhizoctonia solani 4-hydroxyphenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 additional information N-methyl-L-phenylalanine, L-(alpha)-methylphenylalanine, N-acetyl-L-phenylalanine methyl ester, 2-phenylethylamine, N-alpha-acetyl-L-arginine, L-canavanine, L-serine, L-cysteine, L-threonine, D-phenylalanine, L-phenylglycine, (S)-beta-phenylalanine, L-proline, L-tert-leucine, L-valine, and L-alanine are no substrates Rhizoctonia solani ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.2 homodimer 2 * 65000, SDS-PAGE Rhizoctonia solani

Synonyms

EC Number Synonyms Comment Organism
1.4.3.2 L-AAO1
-
 Rhizoctonia solani

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.2 0.08
-
 L-Met at pH 8.5 and 30°C Rhizoctonia solani
1.4.3.2 0.09
-
 L-Lys at pH 8.5 and 30°C Rhizoctonia solani
1.4.3.2 0.1
-
 L-Orn at pH 8.5 and 30°C Rhizoctonia solani
1.4.3.2 0.13
-
 L-Arg at pH 8.5 and 30°C Rhizoctonia solani
1.4.3.2 0.13
-
 L-Leu at pH 8.5 and 30°C Rhizoctonia solani
1.4.3.2 0.16
-
 L-Phe at pH 8.5 and 30°C Rhizoctonia solani
1.4.3.2 0.16
-
 L-phenylalanine methyl ester at pH 8.5 and 30°C Rhizoctonia solani

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.3.2 7 9.5
-
 Rhizoctonia solani

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.2 FAD
-
 Rhizoctonia solani

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.2 0.004
-
 L-Met at pH 8.5 and 30°C Rhizoctonia solani
1.4.3.2 0.01
-
 L-phenylalanine methyl ester at pH 8.5 and 30°C Rhizoctonia solani
1.4.3.2 0.02
-
 L-Phe at pH 8.5 and 30°C Rhizoctonia solani
1.4.3.2 0.02
-
 L-Leu at pH 8.5 and 30°C Rhizoctonia solani
1.4.3.2 0.02
-
 L-Orn at pH 8.5 and 30°C Rhizoctonia solani
1.4.3.2 0.07
-
 L-Lys at pH 8.5 and 30°C Rhizoctonia solani
1.4.3.2 0.3
-
 L-Arg at pH 8.5 and 30°C Rhizoctonia solani