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Literature summary extracted from

  • Katsimpouras, C.; Dimarogona, M.; Petropoulos, P.; Christakopoulos, P.; Topakas, E.
    A thermostable GH26 endo-beta-mannanase from Myceliophthora thermophila capable of enhancing lignocellulose degradation (2016), Appl. Microbiol. Biotechnol., 100, 8385-8397 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.78 degradation the addition of Man26A as a supplement to the commercial enzyme mixture Celluclast 1.5 L and Novozyme 188 results in enhanced enzymatic hydrolysis of pretreated beechwood sawdust, the release of total reducing sugars and glucose is improved by 13 and 12%, respectively Thermothelomyces thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expression in Pichia pastoris Thermothelomyces thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.78 homology modeling of structure Thermothelomyces thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.78 Ag+ 5 mM, complete loss of activity Thermothelomyces thermophilus
3.2.1.78 additional information presence of Ca2+, Na+, Mg2+, Mn2+, urea, and EDTA does not affect catalytic activity Thermothelomyces thermophilus
3.2.1.78 Zn2+ 5 mM, partial loss of activity Thermothelomyces thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.78 additional information
-
 carob galactomannan Km value 2.22 mg/ml, pH 5.0, 60°C Thermothelomyces thermophilus
3.2.1.78 additional information
-
 konjac glucomannan Km value 3.22 mg/ml, pH 5.0, 60°C Thermothelomyces thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.78 Co2+ 5 mM, 123% of initial activity Thermothelomyces thermophilus
3.2.1.78 Cu2+ 5 mM, 121% of initial activity Thermothelomyces thermophilus
3.2.1.78 Ni2+ 5 mM, 115% of initial activity Thermothelomyces thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Thermothelomyces thermophilus G2Q4H7 glycoside hydrolase family 26 protein
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.78 glycoprotein treatment with EndoH leads to a mass reduction of 7000 Da Thermothelomyces thermophilus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 recombinant protein Thermothelomyces thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 182
-
 substrate beta-mannan, pH 5.0, 60°C Thermothelomyces thermophilus
3.2.1.78 220
-
 substrate carob galactomannan, pH 5.0, 60°C Thermothelomyces thermophilus
3.2.1.78 312
-
 substrate konjac glucomannan, pH 5.0, 60°C Thermothelomyces thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 beta-mannan + H2O
-
 Thermothelomyces thermophilus ?
-
 ?
3.2.1.78 carob galactomannan + H2O
-
 Thermothelomyces thermophilus ?
-
 ?
3.2.1.78 konjac glucomannan + H2O
-
 Thermothelomyces thermophilus ?
-
 ?
3.2.1.78 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O
-
 Thermothelomyces thermophilus ?
-
 ?
3.2.1.78 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O
-
 Thermothelomyces thermophilus Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + mannose
-
 ?
3.2.1.78 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O
-
 Thermothelomyces thermophilus Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Man
-
 ?
3.2.1.78 additional information no activity against barley beta-glucan, birchwood xylan, and low viscosity carboxymethyl cellulose Thermothelomyces thermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 60000, SDS-PAGE of recombinant protein, x * 50605, calculated Thermothelomyces thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 em26a
-
 Thermothelomyces thermophilus
3.2.1.78 Man26A
-
 Thermothelomyces thermophilus
3.2.1.78 MYCTH_99077
-
 Thermothelomyces thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 60
-
-
 Thermothelomyces thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 60
-
 half-life 14.4 h Thermothelomyces thermophilus
3.2.1.78 65
-
 half-life 35.3 min Thermothelomyces thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 6
-
-
 Thermothelomyces thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.78 5 9 more than 90% of maximum activity Thermothelomyces thermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.78 4 10 24 h, more than 88% residual activity Thermothelomyces thermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.78 Thermothelomyces thermophilus isoelectric focusing
-
 3.6
3.2.1.78 Thermothelomyces thermophilus calculated
-
 4.4

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.78 additional information
-
 carob galactomannan kcat/Km value 8022 ml/mg/s, pH 5.0, 60°C Thermothelomyces thermophilus
3.2.1.78 additional information
-
 konjac glucomannan kcat/Km value 8855 ml/mg/s, pH 5.0, 60°C Thermothelomyces thermophilus