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Literature summary extracted from

  • Callejon, S.; Sendra, R.; Ferrer, S.; Pardo, I.
    Cloning and characterization of a new laccase from Lactobacillus plantarum J16 CECT 8944 catalyzing biogenic amines degradation (2016), Appl. Microbiol. Biotechnol., 100, 3113-3124 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.10.3.2 degradation eliminating toxic compounds (biogenic amines) present in fermented food and beverages Lactiplantibacillus plantarum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 heterologously overexpressed in Escherichia coli Lactiplantibacillus plantarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide 99.5% inhibition Lactiplantibacillus plantarum
1.10.3.2 bipyridyl 90% inhibition Lactiplantibacillus plantarum
1.10.3.2 additional information no inhibition by 0.1 mM EDTA, laccase retains 100 % activity with 2,6-dimethoxyphenol as a substrate in the presence of clorgiline or pargyline (specific amine oxidase inhibitors) Lactiplantibacillus plantarum
1.10.3.2 Phenanthroline 95% inhibition Lactiplantibacillus plantarum
1.10.3.2 Semicarbazide 25% decrease in enzyme activity Lactiplantibacillus plantarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.21
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 7.4, 37°C Lactiplantibacillus plantarum
1.10.3.2 1.67
-
 2,6-dimethoxyphenol pH 7.4, 37°C Lactiplantibacillus plantarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ multicopper enzyme Lactiplantibacillus plantarum

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Lactiplantibacillus plantarum
-
-
-
1.10.3.2 Lactiplantibacillus plantarum J16 CECT 8944
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Lactiplantibacillus plantarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Lactiplantibacillus plantarum ?
-
 ?
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Lactiplantibacillus plantarum J16 CECT 8944 ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Lactiplantibacillus plantarum ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Lactiplantibacillus plantarum J16 CECT 8944 ?
-
 ?
1.10.3.2 additional information 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) acts as an effective mediator for amine oxidation by the enzyme. Higher concentrations of 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) achieve higher tyramine degradation percentages to nearly 100% with 5 mM Lactiplantibacillus plantarum ?
-
 ?
1.10.3.2 additional information 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) acts as an effective mediator for amine oxidation by the enzyme. Higher concentrations of 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) achieve higher tyramine degradation percentages to nearly 100% with 5 mM Lactiplantibacillus plantarum J16 CECT 8944 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 62500, SDS-PAGE Lactiplantibacillus plantarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 60
-
 substrate: 2,6-dimethoxyphenol Lactiplantibacillus plantarum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 37 100 37°C: about 55% of maximal activity, 100°C: about 60% of maximal activity, substrate: 2,6-dimethoxyphenol Lactiplantibacillus plantarum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 45 70 the enzyme is activated by 10 min of preincubation at 45-70°C, but higher temperatures promote inactivation Lactiplantibacillus plantarum
1.10.3.2 85
-
 10 min, complete inactivation Lactiplantibacillus plantarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3.5
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Lactiplantibacillus plantarum
1.10.3.2 6.5
-
 substrate: 2,6-dimethoxyphenol Lactiplantibacillus plantarum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 3 4 pH 3.0: about 40% of maximal activity, pH 4.0: about 30% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Lactiplantibacillus plantarum
1.10.3.2 6 8 pH 6.0: about 25% of maximal activity, pH 8.0: about 70% of maximal activity, substrate: 2,6-dimethoxyphenol Lactiplantibacillus plantarum