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Literature summary extracted from

  • Wenning, L.; Stoeveken, N.; Wuebbeler, J.H.; Steinbuechel, A.
    Substrate and cofactor range differences of two cysteine dioxygenases from Ralstonia eutropha H16 (2015), Appl. Environ. Microbiol., 82, 910-921 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.20 ethylxanthate 0.002 mM, 110% of initial activity, 0.004 mM, 89% of initial activity Cupriavidus necator
1.13.11.91 ethylxanthate 0.002 mM, 110% of initial activity, 0.004 mM, 89% of initial activity Cupriavidus necator

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.20 expression in Escherichia coli Cupriavidus necator
1.13.11.20 gene H16_A1614, located on chromosome 1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme CdoB in Escherichia coli Cupriavidus necator
1.13.11.20 gene H16_B1863, located on chromosome 2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme CdoA in Escherichia coli. Escherichia coli cells expressing CdoA can convert 3-mercaptopropionate to 3-sulfinopropionate Cupriavidus necator
1.13.11.91 expression in Escherichia coli Cupriavidus necator

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.20 2-mercaptoethanol
-
 Cupriavidus necator
1.13.11.20 3-Mercaptopropionate complete inhibition at 0.05 mM Cupriavidus necator
1.13.11.20 cysteamine
-
 Cupriavidus necator
1.13.11.20 EDTA 0.05 mM, 80% of initial activity, 0.1 mM, 61% of initial activity; 40% inhibition at 0.1 mM, 65% inhibition at 0.2 mM; complete inhibition at 0.05 mM Cupriavidus necator
1.13.11.20 ethyl xanthate 44% inhibition at 0.004 mM Cupriavidus necator
1.13.11.20 ethylxanthate 0.002 mM, 110% of initial activity, 0.004 mM, 89% of initial activity Cupriavidus necator
1.13.11.20 additional information metal ions are inhibitory above 0.2 mM Cupriavidus necator
1.13.11.20 sodium ethylxanthate 11% inhibition at 0.004 mM Cupriavidus necator
1.13.11.91 EDTA 0.05 mM, 80% of initial activity, 0.1 mM, 61% of initial activity Cupriavidus necator
1.13.11.91 ethylxanthate 0.002 mM, 110% of initial activity, 0.004 mM, 89% of initial activity Cupriavidus necator
1.13.11.91 additional information addition of cysteine results in a minor decrease of activity Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.20 2.85
-
 L-cysteine pH 6.5, 30°C, recombinant His-tagged enzyme Cupriavidus necator
1.13.11.20 5.7
-
 3-Mercaptopropionate pH 6.5, 30°C, recombinant His-tagged enzyme Cupriavidus necator
1.13.11.20 6.7
-
 3-Mercaptopropanoate pH 6.5, 30°C Cupriavidus necator
1.13.11.20 6.7
-
 L-cysteine pH 6.5, 30°C, recombinant His-tagged enzyme Cupriavidus necator
1.13.11.91 5.7
-
 3-Mercaptopropanoate pH 6.5, 30°C Cupriavidus necator

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.20 Cu2+ activates Cupriavidus necator
1.13.11.20 Fe2+ 0.38 mol Fe(II) per mol CdoB, activates Cupriavidus necator
1.13.11.20 Fe2+ 0.42 mol Fe(II) per mol CdoB, activates Cupriavidus necator
1.13.11.20 Fe3+ 0.048 mol Fe(III) per mol enzyme Cupriavidus necator
1.13.11.20 Iron iron content of 0.38 mol Fe(II) per mol of enzyme Cupriavidus necator
1.13.11.20 additional information CdoA contains no Fe3+ Cupriavidus necator
1.13.11.20 additional information metal ions are inhibitory above 0.2 mM Cupriavidus necator
1.13.11.20 Ni2+ activates Cupriavidus necator
1.13.11.20 Zn2+ activates Cupriavidus necator
1.13.11.91 Iron iron content of 0.38 mol Fe(II) per mol of enzyme Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.20 46800
-
 gel filtration Cupriavidus necator
1.13.11.20 63900
-
 recombinant His-tagged enzyme CdoB, gel filtration Cupriavidus necator
1.13.11.91 46800
-
 gel filtration Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.20 L-cysteine + O2 Cupriavidus necator
-
 3-sulfinoalanine
-
 ?
1.13.11.20 L-cysteine + O2 Cupriavidus necator DSM 428
-
 3-sulfinoalanine
-
 ?
1.13.11.20 L-cysteine + O2 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
 3-sulfinoalanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.20 Cupriavidus necator Q0K029
-
-
1.13.11.20 Cupriavidus necator Q0K029 cf. EC 1.13.11.91
-
1.13.11.20 Cupriavidus necator Q0KB75
-
-
1.13.11.20 Cupriavidus necator DSM 428 Q0K029 cf. EC 1.13.11.91
-
1.13.11.20 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 Q0K029
-
-
1.13.11.20 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 Q0KB75
-
-
1.13.11.91 Cupriavidus necator Q0K029
-
-
1.13.11.91 Cupriavidus necator DSM 428 Q0K029
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.20 recombinant His-tagged enzyme CdoA from Escherichia coli by nickel affinity chromatography to homogeneity Cupriavidus necator
1.13.11.20 recombinant His-tagged enzyme CdoB from Escherichia coli by nickel affinity chromatography to homogeneity Cupriavidus necator

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.20 0.05
-
 pH 6.5, 30°C Cupriavidus necator
1.13.11.91 2.2
-
 pH 6.5, 30°C Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.20 3-mercaptopropanoate + O2 reaction of EC 1.13.11.91 Cupriavidus necator 3-sulfinopropanoate + H+
-
 ?
1.13.11.20 3-mercaptopropanoate + O2 reaction of EC 1.13.11.91 Cupriavidus necator DSM 428 3-sulfinopropanoate + H+
-
 ?
1.13.11.20 3-mercaptopropanoate + O2 reaction of EC 1.13.11.91 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 3-sulfinopropanoate + H+
-
 ?
1.13.11.20 3-mercaptopropionate + O2 3fold higher activity compared to L-cysteine. 3-Mercaptopropionate does not occur naturally in cells of strain H16 Cupriavidus necator 3-sulfinopropionate
-
 ?
1.13.11.20 3-mercaptopropionate + O2 3fold higher activity compared to L-cysteine. 3-Mercaptopropionate does not occur naturally in cells of strain H16 Cupriavidus necator DSM 428 3-sulfinopropionate
-
 ?
1.13.11.20 3-mercaptopropionate + O2 3fold higher activity compared to L-cysteine. 3-Mercaptopropionate does not occur naturally in cells of strain H16 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 3-sulfinopropionate
-
 ?
1.13.11.20 L-cysteine + O2
-
 Cupriavidus necator 3-sulfinoalanine
-
 ?
1.13.11.20 L-cysteine + O2
-
 Cupriavidus necator DSM 428 3-sulfinoalanine
-
 ?
1.13.11.20 L-cysteine + O2
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 3-sulfinoalanine
-
 ?
1.13.11.20 additional information no activity with 3-mercaptopropionate by CdoB Cupriavidus necator ?
-
 ?
1.13.11.20 additional information 3-mercaptopropanoate is preferred over cysteine. No substrate: cysteamine Cupriavidus necator ?
-
-
1.13.11.20 additional information 3-mercaptopropanoate is preferred over cysteine. No substrate: cysteamine Cupriavidus necator DSM 428 ?
-
-
1.13.11.20 additional information 3-mercaptopropanoate is preferred over cysteine. No substrate: cysteamine Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
-
1.13.11.20 additional information no activity with 3-mercaptopropionate by CdoB Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
 ?
1.13.11.91 3-mercaptopropanoate + O2
-
 Cupriavidus necator 3-sulfinopropanoate
-
 ?
1.13.11.91 L-cysteine + O2 reaction of EC 1.13.11.20 Cupriavidus necator 3-sulfinoalanine
-
 ?
1.13.11.91 additional information 3-mercaptopropanoate is preferred over cysteine. No substrate: cysteamine Cupriavidus necator ?
-
-
1.13.11.91 additional information 3-mercaptopropanoate is preferred over cysteine. No substrate: cysteamine Cupriavidus necator DSM 428 ?
-
-
1.13.11.91 additional information 3-mercaptopropanoate is preferred over cysteine. No substrate: cysteamine Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
-

Subunits

EC Number Subunits Comment Organism
1.13.11.20 ? x * 22580, sequence calculation Cupriavidus necator
1.13.11.20 dimer 2 * 22580, calculated from sequence Cupriavidus necator
1.13.11.20 trimer 3 * 22390, sequence calculation Cupriavidus necator
1.13.11.91 dimer 2 * 22580, calculated from sequence Cupriavidus necator

Synonyms

EC Number Synonyms Comment Organism
1.13.11.20 CdoA
-
 Cupriavidus necator
1.13.11.20 CdoB
-
 Cupriavidus necator
1.13.11.20 H16_A1614
-
 Cupriavidus necator
1.13.11.20 H16_B1863
-
 Cupriavidus necator
1.13.11.91 CdoA
-
 Cupriavidus necator
1.13.11.91 H16_B1863
-
 Cupriavidus necator

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.20 30
-
 assay at Cupriavidus necator

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.20 0.01
-
 3-Mercaptopropanoate pH 6.5, 30°C Cupriavidus necator
1.13.11.20 0.01
-
 L-cysteine pH 6.5, 30°C, recombinant His-tagged enzyme Cupriavidus necator
1.13.11.20 0.25
-
 L-cysteine pH 6.5, 30°C, recombinant His-tagged enzyme Cupriavidus necator
1.13.11.20 0.82
-
 3-Mercaptopropionate pH 6.5, 30°C, recombinant His-tagged enzyme Cupriavidus necator
1.13.11.91 0.82
-
 3-Mercaptopropanoate pH 6.5, 30°C Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.20 6.5
-
-
 Cupriavidus necator
1.13.11.20 6.8
-
-
 Cupriavidus necator
1.13.11.91 6.5
-
-
 Cupriavidus necator

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.11.20 Cupriavidus necator sequence calculation
-
 5.8
1.13.11.20 Cupriavidus necator calculated from sequence
-
 5.8
1.13.11.20 Cupriavidus necator sequence calculation
-
 7
1.13.11.91 Cupriavidus necator calculated from sequence
-
 5.8

General Information

EC Number General Information Comment Organism
1.13.11.20 malfunction deletion of cdoA might enable increased synthesis of polythioesters Cupriavidus necator
1.13.11.20 physiological function importance of CdoA for the metabolism of the sulfur compounds 3,3'-thiodipropionic acid and 3,3'-dithiodipropionic acid by further converting their degradation product, 3-mercaptopropionate Cupriavidus necator

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.20 0.002
-
 3-Mercaptopropanoate pH 6.5, 30°C Cupriavidus necator
1.13.11.20 0.002
-
 L-cysteine pH 6.5, 30°C, recombinant His-tagged enzyme Cupriavidus necator
1.13.11.20 0.087
-
 L-cysteine pH 6.5, 30°C, recombinant His-tagged enzyme Cupriavidus necator
1.13.11.20 0.144
-
 3-Mercaptopropionate pH 6.5, 30°C, recombinant His-tagged enzyme Cupriavidus necator
1.13.11.91 0.144
-
 3-Mercaptopropanoate pH 6.5, 30°C Cupriavidus necator