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Literature summary extracted from

  • Li, H.; Xie, K.; Yu, W.; Hu, L.; Huang, H.; Xie, H.; Wang, S.
    Nicotine dehydrogenase complexed with 6-hydroxypseudooxynicotine oxidase involved in the hybrid nicotine-degrading pathway in Agrobacterium tumefaciens S33 (2016), Appl. Environ. Microbiol., 82, 1745-1755 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.99.14 expressed in Escherichia coli Agrobacterium tumefaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.99.4 Fe2+ the enzyme contains a [2Fe-2S]-center Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.163 4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2 Agrobacterium tumefaciens
-
 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O
-
 ?
1.14.13.163 4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2 Agrobacterium tumefaciens S33
-
 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.4 Agrobacterium tumefaciens
-
-
-
1.5.99.4 Agrobacterium tumefaciens S33
-
-
-
1.5.99.14 Agrobacterium tumefaciens
-
-
-
1.5.99.14 Agrobacterium tumefaciens S33
-
-
-
1.14.13.163 Agrobacterium tumefaciens W8E0Y0
-
-
1.14.13.163 Agrobacterium tumefaciens S33 W8E0Y0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.99.4 ammonium sulfate precipitation, phenyl Sepharose column chromatography, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.4 (S)-nicotine + 2,6-dichlorophenolindophenol + H2O
-
 Agrobacterium tumefaciens (S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.4 (S)-nicotine + 2,6-dichlorophenolindophenol + H2O
-
 Agrobacterium tumefaciens S33 (S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.14 6-hydroxypseudooxynicotine + 2,6-dichlorophenolindophenol + H2O
-
 Agrobacterium tumefaciens 6-hydroxy-3-succinoylsemialdehyde-pyridine + methylamine + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.14 6-hydroxypseudooxynicotine + 2,6-dichlorophenolindophenol + H2O
-
 Agrobacterium tumefaciens S33 6-hydroxy-3-succinoylsemialdehyde-pyridine + methylamine + reduced 2,6-dichlorophenolindophenol
-
 ?
1.14.13.163 4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2
-
 Agrobacterium tumefaciens 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O
-
 ?
1.14.13.163 4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2
-
 Agrobacterium tumefaciens S33 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.99.4 heterodimer 1 * 75000 + 1 * 15000, SDS-PAGE Agrobacterium tumefaciens
1.5.99.4 heterodimer 1 * 82400 + 1 * 17100, calculated from amino acid sequence Agrobacterium tumefaciens
1.5.99.14 ? x * 73349, calculated from amino acid sequence Agrobacterium tumefaciens

Synonyms

EC Number Synonyms Comment Organism
1.5.99.4 NdhAB
-
 Agrobacterium tumefaciens
1.5.99.14 PNO
-
 Agrobacterium tumefaciens
1.14.13.163 6-hydroxy-3-succinoylpyridine hydroxylase
-
 Agrobacterium tumefaciens
1.14.13.163 AWN88_01205 gene name, UniProt Agrobacterium tumefaciens
1.14.13.163 Hsh
-
 Agrobacterium tumefaciens
1.14.13.163 HSP hydroxylase
-
 Agrobacterium tumefaciens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.4 molybdopterin
-
 Agrobacterium tumefaciens
1.5.99.4 [2Fe-2S]-center
-
 Agrobacterium tumefaciens
1.5.99.14 FMN
-
 Agrobacterium tumefaciens
1.5.99.14 [4Fe-4S]-center
-
 Agrobacterium tumefaciens
1.14.13.163 FAD required for activity Agrobacterium tumefaciens
1.14.13.163 NADH dependent on Agrobacterium tumefaciens

General Information

EC Number General Information Comment Organism
1.14.13.163 metabolism 6-hydroxy-3-succinoylpyridine hydroxylase catalyzes a central step of nicotine degradation. 6-Hydroxy-3-succinoylpyridine (HSP) is a key intermediate connecting the two pathways, pyridine pathway and pyrrolidine pathway, detailed overview Agrobacterium tumefaciens
1.14.13.163 physiological function the key enzyme HSP hydroxylase is involved in the fused nicotine degradation pathway of the pyridine and pyrrolidine pathways Agrobacterium tumefaciens