BRENDA - Enzyme Database

Nicotine dehydrogenase complexed with 6-hydroxypseudooxynicotine oxidase involved in the hybrid nicotine-degrading pathway in Agrobacterium tumefaciens S33

Li, H.; Xie, K.; Yu, W.; Hu, L.; Huang, H.; Xie, H.; Wang, S.; Appl. Environ. Microbiol. 82, 1745-1755 (2016)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.5.99.14
expressed in Escherichia coli
Agrobacterium tumefaciens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.5.99.4
Fe2+
the enzyme contains a [2Fe-2S]-center
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.163
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2
Agrobacterium tumefaciens
-
2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O
-
-
?
1.14.13.163
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2
Agrobacterium tumefaciens S33
-
2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.5.99.4
Agrobacterium tumefaciens
-
-
-
1.5.99.4
Agrobacterium tumefaciens S33
-
-
-
1.5.99.14
Agrobacterium tumefaciens
-
-
-
1.5.99.14
Agrobacterium tumefaciens S33
-
-
-
1.14.13.163
Agrobacterium tumefaciens
W8E0Y0
-
-
1.14.13.163
Agrobacterium tumefaciens S33
W8E0Y0
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.5.99.4
ammonium sulfate precipitation, phenyl Sepharose column chromatography, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration
Agrobacterium tumefaciens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.5.99.4
(S)-nicotine + 2,6-dichlorophenolindophenol + H2O
-
741698
Agrobacterium tumefaciens
(S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.5.99.4
(S)-nicotine + 2,6-dichlorophenolindophenol + H2O
-
741698
Agrobacterium tumefaciens S33
(S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.5.99.14
6-hydroxypseudooxynicotine + 2,6-dichlorophenolindophenol + H2O
-
741698
Agrobacterium tumefaciens
6-hydroxy-3-succinoylsemialdehyde-pyridine + methylamine + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.5.99.14
6-hydroxypseudooxynicotine + 2,6-dichlorophenolindophenol + H2O
-
741698
Agrobacterium tumefaciens S33
6-hydroxy-3-succinoylsemialdehyde-pyridine + methylamine + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.14.13.163
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2
-
741698
Agrobacterium tumefaciens
2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O
-
-
-
?
1.14.13.163
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2
-
741698
Agrobacterium tumefaciens S33
2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.5.99.4
heterodimer
1 * 75000 + 1 * 15000, SDS-PAGE; 1 * 82400 + 1 * 17100, calculated from amino acid sequence
Agrobacterium tumefaciens
1.5.99.14
?
x * 73349, calculated from amino acid sequence
Agrobacterium tumefaciens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.5.99.4
molybdopterin
-
Agrobacterium tumefaciens
1.5.99.4
[2Fe-2S]-center
-
Agrobacterium tumefaciens
1.5.99.14
FMN
-
Agrobacterium tumefaciens
1.5.99.14
[4Fe-4S]-center
-
Agrobacterium tumefaciens
1.14.13.163
FAD
required for activity
Agrobacterium tumefaciens
1.14.13.163
NADH
dependent on
Agrobacterium tumefaciens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.5.99.14
expressed in Escherichia coli
Agrobacterium tumefaciens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.5.99.4
molybdopterin
-
Agrobacterium tumefaciens
1.5.99.4
[2Fe-2S]-center
-
Agrobacterium tumefaciens
1.5.99.14
FMN
-
Agrobacterium tumefaciens
1.5.99.14
[4Fe-4S]-center
-
Agrobacterium tumefaciens
1.14.13.163
FAD
required for activity
Agrobacterium tumefaciens
1.14.13.163
NADH
dependent on
Agrobacterium tumefaciens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.5.99.4
Fe2+
the enzyme contains a [2Fe-2S]-center
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.163
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2
Agrobacterium tumefaciens
-
2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O
-
-
?
1.14.13.163
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2
Agrobacterium tumefaciens S33
-
2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.5.99.4
ammonium sulfate precipitation, phenyl Sepharose column chromatography, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration
Agrobacterium tumefaciens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.5.99.4
(S)-nicotine + 2,6-dichlorophenolindophenol + H2O
-
741698
Agrobacterium tumefaciens
(S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.5.99.4
(S)-nicotine + 2,6-dichlorophenolindophenol + H2O
-
741698
Agrobacterium tumefaciens S33
(S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.5.99.14
6-hydroxypseudooxynicotine + 2,6-dichlorophenolindophenol + H2O
-
741698
Agrobacterium tumefaciens
6-hydroxy-3-succinoylsemialdehyde-pyridine + methylamine + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.5.99.14
6-hydroxypseudooxynicotine + 2,6-dichlorophenolindophenol + H2O
-
741698
Agrobacterium tumefaciens S33
6-hydroxy-3-succinoylsemialdehyde-pyridine + methylamine + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.14.13.163
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2
-
741698
Agrobacterium tumefaciens
2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O
-
-
-
?
1.14.13.163
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2
-
741698
Agrobacterium tumefaciens S33
2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.5.99.4
heterodimer
1 * 75000 + 1 * 15000, SDS-PAGE; 1 * 82400 + 1 * 17100, calculated from amino acid sequence
Agrobacterium tumefaciens
1.5.99.14
?
x * 73349, calculated from amino acid sequence
Agrobacterium tumefaciens
General Information
EC Number
General Information
Commentary
Organism
1.14.13.163
metabolism
6-hydroxy-3-succinoylpyridine hydroxylase catalyzes a central step of nicotine degradation. 6-Hydroxy-3-succinoylpyridine (HSP) is a key intermediate connecting the two pathways, pyridine pathway and pyrrolidine pathway, detailed overview
Agrobacterium tumefaciens
1.14.13.163
physiological function
the key enzyme HSP hydroxylase is involved in the fused nicotine degradation pathway of the pyridine and pyrrolidine pathways
Agrobacterium tumefaciens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.13.163
metabolism
6-hydroxy-3-succinoylpyridine hydroxylase catalyzes a central step of nicotine degradation. 6-Hydroxy-3-succinoylpyridine (HSP) is a key intermediate connecting the two pathways, pyridine pathway and pyrrolidine pathway, detailed overview
Agrobacterium tumefaciens
1.14.13.163
physiological function
the key enzyme HSP hydroxylase is involved in the fused nicotine degradation pathway of the pyridine and pyrrolidine pathways
Agrobacterium tumefaciens