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Literature summary extracted from
- A highly stable d-amino acid oxidase of the thermophilic bacterium Rubrobacter xylanophilus (2014), Appl. Environ. Microbiol., 80, 7219-7229 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.3.3 | 2,3-Butanedione | 115% activity at 10 mM | Rubrobacter xylanophilus |  |
| 1.4.3.3 | D-malate | 107% activity at 10 mM | Rubrobacter xylanophilus | |
| 1.4.3.3 | iodoacetate | 112% activity at 10 mM | Rubrobacter xylanophilus | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.4.3.3 | expressed in Escherichia coli BL21(DE3) or BL21 Star(DE3) cells | Rubrobacter xylanophilus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.3.3 | anthranilate | 83.3% residual activity at 10 mM | Rubrobacter xylanophilus | |
| 1.4.3.3 | benzoate | 37% residual activity at 10 mM | Rubrobacter xylanophilus | |
| 1.4.3.3 | crotonate | 89.2% residual activity at 10 mM | Rubrobacter xylanophilus | |
| 1.4.3.3 | D-Tyr | - |
Rubrobacter xylanophilus | |
| 1.4.3.3 | additional information | not inhibited by diethyl pyrocarbonate, malonate, and meso-tartrate | Rubrobacter xylanophilus | |
| 1.4.3.3 | phenylmethylsulfonyl fluoride | 31.8% residual activity at 10 mM | Rubrobacter xylanophilus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.3.3 | 0.049 | - |
D-Leu | at pH 8.0 and 60°C | Rubrobacter xylanophilus | |
| 1.4.3.3 | 0.052 | - |
D-Ile | at pH 8.0 and 60°C | Rubrobacter xylanophilus | |
| 1.4.3.3 | 0.094 | - |
D-Val | at pH 8.0 and 60°C | Rubrobacter xylanophilus | |
| 1.4.3.3 | 0.197 | - |
D-Tyr | at pH 8.0 and 60°C | Rubrobacter xylanophilus | |
| 1.4.3.3 | 8.16 | - |
D-Thr | at pH 8.0 and 60°C | Rubrobacter xylanophilus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.4.3.3 | 24100 | - |
gel filtration | Rubrobacter xylanophilus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.4.3.1 | no activity in Rubrobacter xylanophilus | - |
- |
- |
| 1.4.3.3 | Rubrobacter xylanophilus | A0A077JN67 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.4.3.3 | Talon metal affinity column chromatography, and Superdex 200 gel filtration | Rubrobacter xylanophilus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.4.3.3 | 30°C, 1 week, no loss of activity | Rubrobacter xylanophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.3.3 | D-Ala + H2O + O2 | 1.6% activity compared to D-Val | Rubrobacter xylanophilus | pyruvate + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | D-Arg + H2O + O2 | 1.1% activity compared to D-Val | Rubrobacter xylanophilus | 5-guanidino-2-oxopentanoate + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | D-Gln + H2O + O2 | 1.0% activity compared to D-Val | Rubrobacter xylanophilus | 2-oxoglutaramate + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | D-Glu + H2O + O2 | 5.1% activity compared to D-Val | Rubrobacter xylanophilus | alpha-ketoglutarate + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | D-His + H2O + O2 | 3.6% activity compared to D-Val | Rubrobacter xylanophilus | 3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | D-Ile + H2O + O2 | 26.7% activity compared to D-Val | Rubrobacter xylanophilus | 3-methyl-2-oxopentanoate + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | D-Leu + H2O + O2 | 27.2% activity compared to D-Val | Rubrobacter xylanophilus | 4-methyl-2-oxopentanoate + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | D-Lys + H2O + O2 | 2.4% activity compared to D-Val | Rubrobacter xylanophilus | 6-amino-2-oxohexanoic acid + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | D-Met + H2O + O2 | 2.0% activity compared to D-Val | Rubrobacter xylanophilus | 4-methylthio-2-oxobutanoate + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | D-Phe + H2O + O2 | 2.1% activity compared to D-Val | Rubrobacter xylanophilus | phenylpyruvate + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | D-Ser + H2O + O2 | 1.3% activity compared to D-Val | Rubrobacter xylanophilus | 2-oxo-3-hydroxypropionate + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | D-Thr + H2O + O2 | 11.5% activity compared to D-Val | Rubrobacter xylanophilus | 2-oxo-3-hydroxybutyrate + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | D-Trp + H2O + O2 | 1.5% activity compared to D-Val | Rubrobacter xylanophilus | indol-3-pyruvate + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | D-Tyr + H2O + O2 | 17.6% activity compared to D-Val | Rubrobacter xylanophilus | 4-hydroxyphenylpyruvate + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | D-Val + H2O + O2 | 100% activity | Rubrobacter xylanophilus | 2-oxoisovalerate + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | Gly + H2O + O2 | 1.1% activity compared to D-Val | Rubrobacter xylanophilus | acetate + NH3 + H2O2 | - |
? | |
| 1.4.3.3 | additional information | no activity with D-Pro, D-Cys, D-Asp, L-Val, L-Leu, and L-Ile | Rubrobacter xylanophilus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.4.3.3 | monomer | 1 * 35000, SDS-PAGE | Rubrobacter xylanophilus |
| 1.4.3.3 | monomer | 1 * 35100, calculated from amino acid sequence | Rubrobacter xylanophilus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.4.3.3 | DAO | - |
Rubrobacter xylanophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.4.3.3 | 65 | - |
- |
Rubrobacter xylanophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.4.3.3 | 30 | 64 | 50% of the initial enzymatic activity is lost after 1h at 64°C. The half-lives of the enzyme are 462 h at 30°C and 114 h at 50°C | Rubrobacter xylanophilus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.3.3 | 7.6 | - |
D-Thr | at pH 8.0 and 60°C | Rubrobacter xylanophilus | |
| 1.4.3.3 | 23 | - |
D-Ile | at pH 8.0 and 60°C | Rubrobacter xylanophilus | |
| 1.4.3.3 | 24 | - |
D-Leu | at pH 8.0 and 60°C | Rubrobacter xylanophilus | |
| 1.4.3.3 | 31.4 | - |
D-Val | at pH 8.0 and 60°C | Rubrobacter xylanophilus | |
| 1.4.3.3 | 53 | - |
D-Tyr | at pH 8.0 and 60°C | Rubrobacter xylanophilus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.3.3 | 7.5 | 8 | - |
Rubrobacter xylanophilus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.3.3 | 5 | 8 | the enzyne is stable at a pH 5.0 to 8.0 for 1 h at 55°C | Rubrobacter xylanophilus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.3.3 | FAD | - |
Rubrobacter xylanophilus | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.3.3 | 0.265 | - |
D-Tyr | at pH 8.0 and 60°C | Rubrobacter xylanophilus | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.3.3 | 0.931 | - |
D-Thr | at pH 8.0 and 60°C | Rubrobacter xylanophilus | |
| 1.4.3.3 | 269 | - |
D-Tyr | at pH 8.0 and 60°C | Rubrobacter xylanophilus | |
| 1.4.3.3 | 334 | - |
D-Val | at pH 8.0 and 60°C | Rubrobacter xylanophilus | |
| 1.4.3.3 | 442 | - |
D-Ile | at pH 8.0 and 60°C | Rubrobacter xylanophilus | |
| 1.4.3.3 | 490 | - |
D-Leu | at pH 8.0 and 60°C | Rubrobacter xylanophilus | |
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