EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.167 | gene pnpA, expression of the pnp cluster is detected only under inducing conditions, and pnpABA1CDEFG is a single transcriptional operon, genetic structure, RT-PCR and real-time PCR enzyme expression analysis, recombinant expression of N-terminally His6-tagged enzyme PnpA in Escherichia coli strain Rosetta(DE3)pLysS | Burkholderia sp. |
1.14.13.167 | gene pnpA1, recombinant expression of N-terminally His6-tagged enzyme PnpA in Escherichia coli strain Rosetta(DE3)pLysS | Rhodococcus opacus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.13.167 | additional information | construction of pK18mobsacB-pnpA1 for gene knockout of pnpA1 in strain RKJ300 | Rhodococcus opacus |
1.14.13.167 | additional information | enzyme deletion strain SJ98DELTApnpA1 exhibits specific activities for 2-chloro-4-nitrophenol approximately 20% lower than those of wild-type SJ98 | Burkholderia sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.167 | additional information | - |
additional information | Michaelis-Menten kinetics | Burkholderia sp. | |
1.14.13.167 | 0.0062 | - |
2-chloro-4-nitrophenol | pH 7.4, 30°C, recombinant enzyme | Burkholderia sp. | |
1.14.13.167 | 0.0254 | - |
4-nitrophenol | pH 7.4, 30°C, recombinant enzyme | Burkholderia sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.29 | 4-nitrophenol + NAD(P)H + H+ + O2 | Rhodococcus opacus | - |
4-nitrocatechol + NAD(P)+ + H2O | - |
? | |
1.14.13.29 | 4-nitrophenol + NAD(P)H + H+ + O2 | Rhodococcus opacus RKJ300 | - |
4-nitrocatechol + NAD(P)+ + H2O | - |
? | |
1.14.13.167 | 2-chloro-4-nitrophenol + NADPH + H+ + O2 | Rhodococcus opacus | - |
2-chloro-1,4-benzoquinone + nitrite + NADP+ + H2O | - |
? | |
1.14.13.167 | 2-chloro-4-nitrophenol + NADPH + H+ + O2 | Burkholderia sp. | - |
2-chloro-1,4-benzoquinone + nitrite + NADP+ + H2O | - |
? | |
1.14.13.167 | 2-chloro-4-nitrophenol + NADPH + H+ + O2 | Rhodococcus opacus RKJ300 | - |
2-chloro-1,4-benzoquinone + nitrite + NADP+ + H2O | - |
? | |
1.14.13.167 | 4-nitrophenol + NADPH + H+ + O2 | Rhodococcus opacus | - |
1,4-benzoquinone + nitrite + NADP+ + H2O | - |
? | |
1.14.13.167 | 4-nitrophenol + NADPH + H+ + O2 | Burkholderia sp. | - |
1,4-benzoquinone + nitrite + NADP+ + H2O | - |
? | |
1.14.13.167 | 4-nitrophenol + NADPH + H+ + O2 | Rhodococcus opacus RKJ300 | - |
1,4-benzoquinone + nitrite + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.6.5.6 | Burkholderia sp. SJ98 | - |
- |
- |
1.14.13.29 | Rhodococcus opacus | - |
- |
- |
1.14.13.29 | Rhodococcus opacus RKJ300 | - |
- |
- |
1.14.13.167 | Burkholderia sp. | - |
- |
- |
1.14.13.167 | Rhodococcus opacus | - |
- |
- |
1.14.13.167 | Rhodococcus opacus RKJ300 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.167 | recombinant N-terminally His6-tagged enzyme PnpA from Escherichia coli strain Rosetta(DE3)pLysS by nickel affinity chromatography | Rhodococcus opacus |
1.14.13.167 | recombinant N-terminally His6-tagged enzyme PnpA from Escherichia coli strain Rosetta(DE3)pLysS by nickel affinity chromatography | Burkholderia sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.167 | 1.73 | - |
mutant strain SJ98DELTApnpA1 cell extract, pH 7.5, 30°C, substrate 2-chloro-4-nitrophenol | Burkholderia sp. |
1.14.13.167 | 2.1 | - |
cell extract, pH 7.4, 30°C, substrate 2-chloro-4-nitrophenol | Burkholderia sp. |
1.14.13.167 | 3.74 | - |
mutant strain SJ98DELTApnpA1 cell extract, pH 7.5, 30°C, substrate 4-nitrophenol | Burkholderia sp. |
1.14.13.167 | 4.8 | - |
cell extract, pH 7.4, 30°C, substrate 4-nitrophenol | Burkholderia sp. |
1.14.13.167 | 5.6 | - |
pH 7.4, 30°C, substrate 4-nitrophenol | Burkholderia sp. |
1.14.13.167 | 7.2 | - |
pH 7.4, 30°C, substrate 2-chloro-4-nitrophenol | Burkholderia sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.5.6 | NADPH + H+ + chloro-1,4-benzoquinone | - |
Burkholderia sp. SJ98 | NADP+ + chlorohydroquinone | - |
? | |
1.6.5.6 | NADPH + H+ + p-benzoquinone | - |
Burkholderia sp. SJ98 | NADP+ + hydroquinone | - |
? | |
1.14.13.29 | 4-nitrophenol + NAD(P)H + H+ + O2 | - |
Rhodococcus opacus | 4-nitrocatechol + NAD(P)+ + H2O | - |
? | |
1.14.13.29 | 4-nitrophenol + NAD(P)H + H+ + O2 | - |
Rhodococcus opacus RKJ300 | 4-nitrocatechol + NAD(P)+ + H2O | - |
? | |
1.14.13.167 | 2-chloro-4-nitrophenol + NADPH + H+ + O2 | - |
Rhodococcus opacus | 2-chloro-1,4-benzoquinone + nitrite + NADP+ + H2O | - |
? | |
1.14.13.167 | 2-chloro-4-nitrophenol + NADPH + H+ + O2 | - |
Burkholderia sp. | 2-chloro-1,4-benzoquinone + nitrite + NADP+ + H2O | - |
? | |
1.14.13.167 | 2-chloro-4-nitrophenol + NADPH + H+ + O2 | - |
Rhodococcus opacus RKJ300 | 2-chloro-1,4-benzoquinone + nitrite + NADP+ + H2O | - |
? | |
1.14.13.167 | 4-nitrophenol + NADPH + H+ + O2 | - |
Rhodococcus opacus | 1,4-benzoquinone + nitrite + NADP+ + H2O | - |
? | |
1.14.13.167 | 4-nitrophenol + NADPH + H+ + O2 | - |
Burkholderia sp. | 1,4-benzoquinone + nitrite + NADP+ + H2O | - |
? | |
1.14.13.167 | 4-nitrophenol + NADPH + H+ + O2 | - |
Rhodococcus opacus RKJ300 | 1,4-benzoquinone + nitrite + NADP+ + H2O | - |
? | |
1.14.13.167 | additional information | PnpA1A2 also convert 2-chloro-1,4-benzoquinone to 2-hydroxy-4-nitrophenol, the combined isozymes also show low activity with chlorohydroquinone forming 2-hydroxy-1,4-hydroquinone, metabolite identification by GC-MS analysis, overview. Isozyme PnpA2 is not active with 2-chloro-4-nitrophenol | Rhodococcus opacus | ? | - |
? | |
1.14.13.167 | additional information | PnpA1A2 also convert 2-chloro-1,4-benzoquinone to 2-hydroxy-4-nitrophenol, the combined isozymes also show low activity with chlorohydroquinone forming 2-hydroxy-1,4-hydroquinone, metabolite identification by GC-MS analysis, overview. Isozyme PnpA2 is not active with 2-chloro-4-nitrophenol | Rhodococcus opacus RKJ300 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.6.5.6 | PnpB | - |
Burkholderia sp. SJ98 |
1.14.13.167 | 2C4NP 4-monooxygenase | - |
Rhodococcus opacus |
1.14.13.167 | 2C4NP 4-monooxygenase | - |
Burkholderia sp. |
1.14.13.167 | PNP 4-monooxygenase | - |
Rhodococcus opacus |
1.14.13.167 | PNP 4-monooxygenase | - |
Burkholderia sp. |
1.14.13.167 | pnpA | - |
Burkholderia sp. |
1.14.13.167 | PnpA1 | - |
Rhodococcus opacus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.167 | 30 | - |
assay at | Rhodococcus opacus |
1.14.13.167 | 30 | - |
assay at | Burkholderia sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.167 | 7 | - |
assay at | Rhodococcus opacus |
1.14.13.167 | 7.4 | - |
assay at | Burkholderia sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.29 | NAD(P)H | - |
Rhodococcus opacus | |
1.14.13.167 | FAD | - |
Rhodococcus opacus | |
1.14.13.167 | FAD | - |
Burkholderia sp. | |
1.14.13.167 | NADPH | - |
Rhodococcus opacus | |
1.14.13.167 | NADPH | - |
Burkholderia sp. |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.14.13.167 | Burkholderia sp. | 2-chloro-4-nitrophenol degradation is induced by either 2-chloro-4-nitrophenol or 4-nitrophenol, negligible activity in uninduced cells | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.6.5.6 | physiological function | enzyme is able to enhance 4-nitrophenol 4-monooxygenase PnpA activity in vitro in the conversion of 2-chloro-4-nitrophenol to chloro-1,4-benzoquinone | Burkholderia sp. SJ98 |
1.14.13.167 | metabolism | the enzymes responsible for 4-nitrophenol catabolism are also likely to be involved in 2-chloro-4-nitrophenol degradation. Catabolism of 2-chloro-4-nitrophenol and of 4-nitrophenolshare the same gene cluster in strain SJ98 | Burkholderia sp. |
1.14.13.167 | physiological function | PnpA, a PNP 4-monooxygenase, is able to catalyze the monooxygenation of 2-chloro-4-nitrophenol to 2-chloro-1,4-benzoquinone. PnpB, a 1,4-benzoquinone reductase, has the ability to catalyze the reduction of 2-chloro-1,4-benzoquinone to chlorohydroquinone. Moreover, PnpB is also able to enhance PnpA activity in vitro in the conversion of 2-chloro-4-nitrophenol to 2-chloro-1,4-benzoquinone. Gene pnpA plays an essential role in the degradation of both 2-chloro-4-nitrophenol and 4-nitrophenol. Catabolism of 2-chloro-4-nitrophenol and of 4-nitrophenolshare the same gene cluster in strain SJ98 | Burkholderia sp. |
1.14.13.167 | physiological function | PnpA1, a PNP 4-monooxygenase, is able to catalyze the monooxygenation of 2-chloro-4-nitrophenol to 2-chloro-1,4-benzoquinone | Rhodococcus opacus |