BRENDA - Enzyme Database

Novel three-component Rieske non-heme iron oxygenase system catalyzing the N-dealkylation of chloroacetanilide herbicides in sphingomonads DC-6 and DC-2

Chen, Q.; Wang, C.H.; Deng, S.K.; Wu, Y.D.; Li, Y.; Yao, L.; Jiang, J.D.; Yan, X.; He, J.; Li, S.P.; Appl. Environ. Microbiol. 80, 5078-5085 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.15.23
expressed in Escherichia coli BL21(DE3) cells
Sphingobium quisquiliarum
1.14.15.23
expressed in Escherichia coli BL21(DE3) cells
Sphingomonas wittichii
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.15.23
Ag+
severely inhibits enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Ag+
severely inhibits enzyme activity
Sphingomonas wittichii
1.14.15.23
Cu2+
severely inhibits enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Cu2+
severely inhibits enzyme activity
Sphingomonas wittichii
1.14.15.23
EDTA
significantly inhibits enzyme activity
Sphingobium quisquiliarum
1.14.15.23
EDTA
significantly inhibits enzyme activity
Sphingomonas wittichii
1.14.15.23
Hg2+
severely inhibits enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Hg2+
severely inhibits enzyme activity
Sphingomonas wittichii
1.14.15.23
Ni2+
severely inhibits enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Ni2+
severely inhibits enzyme activity
Sphingomonas wittichii
1.14.15.23
Pb2+
severely inhibits enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Pb2+
severely inhibits enzyme activity
Sphingomonas wittichii
1.14.15.23
Zn2+
severely inhibits enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Zn2+
severely inhibits enzyme activity
Sphingomonas wittichii
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.15.23
Ca2+
moderately stimulates enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Ca2+
moderately stimulates enzyme activity
Sphingomonas wittichii
1.14.15.23
Co2+
moderately stimulates enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Co2+
moderately stimulates enzyme activity
Sphingomonas wittichii
1.14.15.23
Cr2+
moderately stimulates enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Cr2+
moderately stimulates enzyme activity
Sphingomonas wittichii
1.14.15.23
Fe2+
stimulates enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Fe2+
stimulates enzyme activity
Sphingomonas wittichii
1.14.15.23
Mg2+
stimulates enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Mg2+
stimulates enzyme activity
Sphingomonas wittichii
1.14.15.23
Mn2+
moderately stimulates enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Mn2+
moderately stimulates enzyme activity
Sphingomonas wittichii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.15.23
Sphingobium quisquiliarum
-
-
-
1.14.15.23
Sphingobium quisquiliarum DC-2
-
-
-
1.14.15.23
Sphingomonas wittichii
A0A059WLZ7
-
-
1.14.15.23
Sphingomonas wittichii DC-6
A0A059WLZ7
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.15.23
Ni-affinity column chromatography
Sphingobium quisquiliarum
1.14.15.23
Ni-affinity column chromatography
Sphingomonas wittichii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.23
acetochlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingomonas wittichii
2-chloro-N-(2,6-diethylphenyl)acetamide + acetyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
acetochlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingobium quisquiliarum
2-chloro-N-(2,6-diethylphenyl)acetamide + acetyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
acetochlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingomonas wittichii DC-6
2-chloro-N-(2,6-diethylphenyl)acetamide + acetyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
acetochlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingobium quisquiliarum DC-2
2-chloro-N-(2,6-diethylphenyl)acetamide + acetyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
alachlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingomonas wittichii
2-chloro-N-(2,6-diethylphenyl)acetamide + allyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
alachlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingobium quisquiliarum
2-chloro-N-(2,6-diethylphenyl)acetamide + allyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
alachlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingomonas wittichii DC-6
2-chloro-N-(2,6-diethylphenyl)acetamide + allyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
alachlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingobium quisquiliarum DC-2
2-chloro-N-(2,6-diethylphenyl)acetamide + allyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
butachlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingomonas wittichii
2-chloro-N-(2,6-diethylphenyl)acetamide + butyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
butachlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingobium quisquiliarum
2-chloro-N-(2,6-diethylphenyl)acetamide + butyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
butachlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingomonas wittichii DC-6
2-chloro-N-(2,6-diethylphenyl)acetamide + butyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
butachlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingobium quisquiliarum DC-2
2-chloro-N-(2,6-diethylphenyl)acetamide + butyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.15.23
35
-
-
Sphingobium quisquiliarum
1.14.15.23
35
-
-
Sphingomonas wittichii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.15.23
7
-
-
Sphingobium quisquiliarum
1.14.15.23
7
-
-
Sphingomonas wittichii
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.23
NADH
NADH, but not NADPH, supports the enzyme activity
Sphingobium quisquiliarum
1.14.15.23
NADH
NADH, but not NADPH, supports the enzyme activity
Sphingomonas wittichii
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.23
expressed in Escherichia coli BL21(DE3) cells
Sphingobium quisquiliarum
1.14.15.23
expressed in Escherichia coli BL21(DE3) cells
Sphingomonas wittichii
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.23
NADH
NADH, but not NADPH, supports the enzyme activity
Sphingobium quisquiliarum
1.14.15.23
NADH
NADH, but not NADPH, supports the enzyme activity
Sphingomonas wittichii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.15.23
Ag+
severely inhibits enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Ag+
severely inhibits enzyme activity
Sphingomonas wittichii
1.14.15.23
Cu2+
severely inhibits enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Cu2+
severely inhibits enzyme activity
Sphingomonas wittichii
1.14.15.23
EDTA
significantly inhibits enzyme activity
Sphingobium quisquiliarum
1.14.15.23
EDTA
significantly inhibits enzyme activity
Sphingomonas wittichii
1.14.15.23
Hg2+
severely inhibits enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Hg2+
severely inhibits enzyme activity
Sphingomonas wittichii
1.14.15.23
Ni2+
severely inhibits enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Ni2+
severely inhibits enzyme activity
Sphingomonas wittichii
1.14.15.23
Pb2+
severely inhibits enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Pb2+
severely inhibits enzyme activity
Sphingomonas wittichii
1.14.15.23
Zn2+
severely inhibits enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Zn2+
severely inhibits enzyme activity
Sphingomonas wittichii
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.15.23
Ca2+
moderately stimulates enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Ca2+
moderately stimulates enzyme activity
Sphingomonas wittichii
1.14.15.23
Co2+
moderately stimulates enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Co2+
moderately stimulates enzyme activity
Sphingomonas wittichii
1.14.15.23
Cr2+
moderately stimulates enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Cr2+
moderately stimulates enzyme activity
Sphingomonas wittichii
1.14.15.23
Fe2+
stimulates enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Fe2+
stimulates enzyme activity
Sphingomonas wittichii
1.14.15.23
Mg2+
stimulates enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Mg2+
stimulates enzyme activity
Sphingomonas wittichii
1.14.15.23
Mn2+
moderately stimulates enzyme activity
Sphingobium quisquiliarum
1.14.15.23
Mn2+
moderately stimulates enzyme activity
Sphingomonas wittichii
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.23
Ni-affinity column chromatography
Sphingobium quisquiliarum
1.14.15.23
Ni-affinity column chromatography
Sphingomonas wittichii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.23
acetochlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingomonas wittichii
2-chloro-N-(2,6-diethylphenyl)acetamide + acetyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
acetochlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingobium quisquiliarum
2-chloro-N-(2,6-diethylphenyl)acetamide + acetyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
acetochlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingomonas wittichii DC-6
2-chloro-N-(2,6-diethylphenyl)acetamide + acetyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
acetochlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingobium quisquiliarum DC-2
2-chloro-N-(2,6-diethylphenyl)acetamide + acetyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
alachlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingomonas wittichii
2-chloro-N-(2,6-diethylphenyl)acetamide + allyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
alachlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingobium quisquiliarum
2-chloro-N-(2,6-diethylphenyl)acetamide + allyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
alachlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingomonas wittichii DC-6
2-chloro-N-(2,6-diethylphenyl)acetamide + allyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
alachlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingobium quisquiliarum DC-2
2-chloro-N-(2,6-diethylphenyl)acetamide + allyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
butachlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingomonas wittichii
2-chloro-N-(2,6-diethylphenyl)acetamide + butyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
butachlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingobium quisquiliarum
2-chloro-N-(2,6-diethylphenyl)acetamide + butyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
butachlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingomonas wittichii DC-6
2-chloro-N-(2,6-diethylphenyl)acetamide + butyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.23
butachlor + reduced ferredoxin [iron-sulfur] cluster + O2
-
741685
Sphingobium quisquiliarum DC-2
2-chloro-N-(2,6-diethylphenyl)acetamide + butyl formate + reduced ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.15.23
35
-
-
Sphingobium quisquiliarum
1.14.15.23
35
-
-
Sphingomonas wittichii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.15.23
7
-
-
Sphingobium quisquiliarum
1.14.15.23
7
-
-
Sphingomonas wittichii
General Information
EC Number
General Information
Commentary
Organism
1.14.15.23
metabolism
the enzyme is involved in the N-dealkylation of chloroacetanilide herbicides
Sphingobium quisquiliarum
1.14.15.23
metabolism
the enzyme is involved in the N-dealkylation of chloroacetanilide herbicides
Sphingomonas wittichii
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.15.23
metabolism
the enzyme is involved in the N-dealkylation of chloroacetanilide herbicides
Sphingobium quisquiliarum
1.14.15.23
metabolism
the enzyme is involved in the N-dealkylation of chloroacetanilide herbicides
Sphingomonas wittichii