EC Number | Cloned (Comment) | Organism |
---|---|---|
1.7.2.5 | genes norC and norB encoding the two enzyme subunits of enzyme cNor, and gene norH, a third gene enclosed into the gene cluster that is cotranscribed in a single mRNA from an inducible promoter. NorH constitutes a third subunit of the cNor, recombinant expression of His-tagged isolated NorH, of His-tagged wild-type enzyme cNOR, and of His-tagged NorH-deficient cNOR in Escherichia coli | Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.7.2.5 | additional information | - |
additional information | steady-state kinetics of recombinant wild-type and mutant enzymes | Thermus thermophilus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.7.2.5 | membrane | cNOR enzyme is integral to the membrane | Thermus thermophilus | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.7.2.5 | Fe2+ | heme | Thermus thermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.2.5 | 2 nitric oxide + 2 ferrocytochrome c + 2 H+ | Thermus thermophilus | - |
nitrous oxide + 2 ferricytochrome c + H2O | - |
? | |
1.7.2.5 | 2 nitric oxide + 2 ferrocytochrome c + 2 H+ | Thermus thermophilus PRQ25 | - |
nitrous oxide + 2 ferricytochrome c + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.7.2.5 | Thermus thermophilus | D5GU62 AND D5GU63 | genes norC and norB encoding the two enzyme subunits | - |
1.7.2.5 | Thermus thermophilus PRQ25 | D5GU62 AND D5GU63 | genes norC and norB encoding the two enzyme subunits | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.7.2.5 | recombinant His-tagged NorH, wild-type cNOR, and mutant cNOR lacking norH by nickel affinity chromatography from Escherichia coli | Thermus thermophilus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.7.2.5 | additional information | Distribution of nor genes in diverse Thermus thermophilus strains, overview | Thermus thermophilus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.2.5 | 2 nitric oxide + 2 ferrocytochrome c + 2 H+ | - |
Thermus thermophilus | nitrous oxide + 2 ferricytochrome c + H2O | - |
? | |
1.7.2.5 | 2 nitric oxide + 2 ferrocytochrome c + 2 H+ | - |
Thermus thermophilus PRQ25 | nitrous oxide + 2 ferricytochrome c + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.7.2.5 | cNOR | - |
Thermus thermophilus |
1.7.2.5 | cytochrome c type NOR | - |
Thermus thermophilus |
1.7.2.5 | cytochrome c-dependent nitric oxide reductase | - |
Thermus thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.7.2.5 | 42 | 60 | assay at | Thermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.7.2.5 | 7.7 | - |
assay at | Thermus thermophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.7.2.5 | cytochrome c | heme containing, dependent on, cytochrome c type NO reductase | Thermus thermophilus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.7.2.5 | evolution | the Nor enzymes belong to the same heme-copper oxidase superfamily as the O2-reducing cytochrome oxidases | Thermus thermophilus |
1.7.2.5 | metabolism | reduction of NO to N2O by denitrifiying bacteria is catalyzed either by a monomeric quinol-nitric oxide reductase (qNor, EC 1.7.5.2) or by a heterodimeric cytochrome c-dependent nitric oxide reductase (cNor), composed of three subunits encoded on single genes in one cotranscribed gene cluster, direct interactions between NorH and the NorCB complex. NOR are the main enzymes involved in the reduction of NO during the denitrification process | Thermus thermophilus |