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Literature summary extracted from

  • Bricio, C.; Alvarez, L.; San Martin, M.; Schurig-Briccio, L.A.; Gennis, R.B.; Berenguer, J.
    A third subunit in ancestral cytochrome c-dependent nitric oxide reductases (2014), Appl. Environ. Microbiol., 80, 4871-4878 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.2.5 genes norC and norB encoding the two enzyme subunits of enzyme cNor, and gene norH, a third gene enclosed into the gene cluster that is cotranscribed in a single mRNA from an inducible promoter. NorH constitutes a third subunit of the cNor, recombinant expression of His-tagged isolated NorH, of His-tagged wild-type enzyme cNOR, and of His-tagged NorH-deficient cNOR in Escherichia coli Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.5 additional information
-
additional information steady-state kinetics of recombinant wild-type and mutant enzymes Thermus thermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.2.5 membrane cNOR enzyme is integral to the membrane Thermus thermophilus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.5 Fe2+ heme Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.5 2 nitric oxide + 2 ferrocytochrome c + 2 H+ Thermus thermophilus
-
nitrous oxide + 2 ferricytochrome c + H2O
-
?
1.7.2.5 2 nitric oxide + 2 ferrocytochrome c + 2 H+ Thermus thermophilus PRQ25
-
nitrous oxide + 2 ferricytochrome c + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.5 Thermus thermophilus D5GU62 AND D5GU63 genes norC and norB encoding the two enzyme subunits
-
1.7.2.5 Thermus thermophilus PRQ25 D5GU62 AND D5GU63 genes norC and norB encoding the two enzyme subunits
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.5 recombinant His-tagged NorH, wild-type cNOR, and mutant cNOR lacking norH by nickel affinity chromatography from Escherichia coli Thermus thermophilus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.2.5 additional information Distribution of nor genes in diverse Thermus thermophilus strains, overview Thermus thermophilus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.5 2 nitric oxide + 2 ferrocytochrome c + 2 H+
-
Thermus thermophilus nitrous oxide + 2 ferricytochrome c + H2O
-
?
1.7.2.5 2 nitric oxide + 2 ferrocytochrome c + 2 H+
-
Thermus thermophilus PRQ25 nitrous oxide + 2 ferricytochrome c + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.7.2.5 cNOR
-
Thermus thermophilus
1.7.2.5 cytochrome c type NOR
-
Thermus thermophilus
1.7.2.5 cytochrome c-dependent nitric oxide reductase
-
Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.2.5 42 60 assay at Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.5 7.7
-
assay at Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.5 cytochrome c heme containing, dependent on, cytochrome c type NO reductase Thermus thermophilus

General Information

EC Number General Information Comment Organism
1.7.2.5 evolution the Nor enzymes belong to the same heme-copper oxidase superfamily as the O2-reducing cytochrome oxidases Thermus thermophilus
1.7.2.5 metabolism reduction of NO to N2O by denitrifiying bacteria is catalyzed either by a monomeric quinol-nitric oxide reductase (qNor, EC 1.7.5.2) or by a heterodimeric cytochrome c-dependent nitric oxide reductase (cNor), composed of three subunits encoded on single genes in one cotranscribed gene cluster, direct interactions between NorH and the NorCB complex. NOR are the main enzymes involved in the reduction of NO during the denitrification process Thermus thermophilus