Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Inoue, K.; Usami, Y.; Ashikawa, Y.; Noguchi, H.; Umeda, T.; Yamagami-Ashikawa, A.; Horisaki, T.; Uchimura, H.; Terada, T.; Nakamura, S.; Shimizu, K.; Habe, H.; Yamane, H.; Fujimoto, Z.; Nojiri, H.
    Structural basis of the divergent oxygenation reactions catalyzed by the rieske nonheme iron oxygenase carbazole 1,9a-dioxygenase (2014), Appl. Environ. Microbiol., 80, 2821-2832 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.22 expressed in Escherichia coli BL21(DE3) cells Janthinobacterium sp. J3

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.12.22 hanging drop vapor diffusion method, using 0.1M ammonium acetate, 12.5% (w/v) polyethylene glycol 3350 in 0.05 M MES at pH 5.5-5.7 Janthinobacterium sp. J3

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.22 F275W the mutant catalyzes the lateral dioxygenation of carbazole with higher yields than the wild type enzyme Janthinobacterium sp. J3
1.14.12.22 I262V the mutant catalyzes the lateral dioxygenation of carbazole with higher yields than the wild type enzyme Janthinobacterium sp. J3
1.14.12.22 Q282Y the mutant catalyzes the lateral dioxygenation of carbazole with higher yields than the wild type enzyme Janthinobacterium sp. J3

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.12.22 0.0507
-
9H-carbazole mutant enzyme I262V, at pH 7.5 and 30°C Janthinobacterium sp. J3
1.14.12.22 0.0546
-
9H-carbazole mutant enzyme Q282N, at pH 7.5 and 30°C Janthinobacterium sp. J3
1.14.12.22 0.0548
-
9H-carbazole mutant enzyme Q282Y, at pH 7.5 and 30°C Janthinobacterium sp. J3

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.22 9H-carbazole + NADH + H+ + O2 Janthinobacterium sp. J3
-
2'-aminobiphenyl-2,3-diol + NAD+
-
?
1.14.12.22 9H-carbazole + NADPH + H+ + O2 Janthinobacterium sp. J3
-
2'-aminobiphenyl-2,3-diol + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.22 Janthinobacterium sp. J3 Q84II6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.22 HiTrap chelation column chromatography and Superdex 200 gel filtration Janthinobacterium sp. J3

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.22 9H-carbazole + NADH + H+ + O2
-
Janthinobacterium sp. J3 2'-aminobiphenyl-2,3-diol + NAD+
-
?
1.14.12.22 9H-carbazole + NADPH + H+ + O2
-
Janthinobacterium sp. J3 2'-aminobiphenyl-2,3-diol + NADP+
-
?
1.14.12.22 anthracene + NADH + H+ + O2
-
Janthinobacterium sp. J3 ?
-
?
1.14.12.22 biphenyl + NADH + H+ + O2
-
Janthinobacterium sp. J3 ?
-
?
1.14.12.22 dibenzothiophene + NADH + H+ + O2
-
Janthinobacterium sp. J3 ?
-
?
1.14.12.22 dibenzo[1,4]dioxin + NADH + H+ + O2
-
Janthinobacterium sp. J3 ?
-
?
1.14.12.22 fluorene + NADH + H+ + O2
-
Janthinobacterium sp. J3 ?
-
?
1.14.12.22 naphthalene + NADH + H+ + O2
-
Janthinobacterium sp. J3 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.22 CARDO
-
Janthinobacterium sp. J3

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.12.22 4
-
9H-carbazole mutant enzyme Q282N, at pH 7.5 and 30°C Janthinobacterium sp. J3
1.14.12.22 4.62
-
9H-carbazole mutant enzyme I262V, at pH 7.5 and 30°C Janthinobacterium sp. J3
1.14.12.22 5.49
-
9H-carbazole mutant enzyme Q282Y, at pH 7.5 and 30°C Janthinobacterium sp. J3

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.22 NADH
-
Janthinobacterium sp. J3
1.14.12.22 NADPH
-
Janthinobacterium sp. J3
1.14.12.22 [2Fe-2S]-center
-
Janthinobacterium sp. J3

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.12.22 0.000733
-
9H-carbazole mutant enzyme Q282N, at pH 7.5 and 30°C Janthinobacterium sp. J3
1.14.12.22 0.00091
-
9H-carbazole mutant enzyme I262V, at pH 7.5 and 30°C Janthinobacterium sp. J3
1.14.12.22 0.001
-
9H-carbazole mutant enzyme Q282Y, at pH 7.5 and 30°C Janthinobacterium sp. J3