EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.12.22 | expressed in Escherichia coli BL21(DE3) cells | Janthinobacterium sp. J3 |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.14.12.22 | hanging drop vapor diffusion method, using 0.1M ammonium acetate, 12.5% (w/v) polyethylene glycol 3350 in 0.05 M MES at pH 5.5-5.7 | Janthinobacterium sp. J3 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.12.22 | F275W | the mutant catalyzes the lateral dioxygenation of carbazole with higher yields than the wild type enzyme | Janthinobacterium sp. J3 |
1.14.12.22 | I262V | the mutant catalyzes the lateral dioxygenation of carbazole with higher yields than the wild type enzyme | Janthinobacterium sp. J3 |
1.14.12.22 | Q282Y | the mutant catalyzes the lateral dioxygenation of carbazole with higher yields than the wild type enzyme | Janthinobacterium sp. J3 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.12.22 | 0.0507 | - |
9H-carbazole | mutant enzyme I262V, at pH 7.5 and 30°C | Janthinobacterium sp. J3 | |
1.14.12.22 | 0.0546 | - |
9H-carbazole | mutant enzyme Q282N, at pH 7.5 and 30°C | Janthinobacterium sp. J3 | |
1.14.12.22 | 0.0548 | - |
9H-carbazole | mutant enzyme Q282Y, at pH 7.5 and 30°C | Janthinobacterium sp. J3 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.12.22 | 9H-carbazole + NADH + H+ + O2 | Janthinobacterium sp. J3 | - |
2'-aminobiphenyl-2,3-diol + NAD+ | - |
? | |
1.14.12.22 | 9H-carbazole + NADPH + H+ + O2 | Janthinobacterium sp. J3 | - |
2'-aminobiphenyl-2,3-diol + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.12.22 | Janthinobacterium sp. J3 | Q84II6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.12.22 | HiTrap chelation column chromatography and Superdex 200 gel filtration | Janthinobacterium sp. J3 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.12.22 | 9H-carbazole + NADH + H+ + O2 | - |
Janthinobacterium sp. J3 | 2'-aminobiphenyl-2,3-diol + NAD+ | - |
? | |
1.14.12.22 | 9H-carbazole + NADPH + H+ + O2 | - |
Janthinobacterium sp. J3 | 2'-aminobiphenyl-2,3-diol + NADP+ | - |
? | |
1.14.12.22 | anthracene + NADH + H+ + O2 | - |
Janthinobacterium sp. J3 | ? | - |
? | |
1.14.12.22 | biphenyl + NADH + H+ + O2 | - |
Janthinobacterium sp. J3 | ? | - |
? | |
1.14.12.22 | dibenzothiophene + NADH + H+ + O2 | - |
Janthinobacterium sp. J3 | ? | - |
? | |
1.14.12.22 | dibenzo[1,4]dioxin + NADH + H+ + O2 | - |
Janthinobacterium sp. J3 | ? | - |
? | |
1.14.12.22 | fluorene + NADH + H+ + O2 | - |
Janthinobacterium sp. J3 | ? | - |
? | |
1.14.12.22 | naphthalene + NADH + H+ + O2 | - |
Janthinobacterium sp. J3 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.12.22 | CARDO | - |
Janthinobacterium sp. J3 |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.12.22 | 4 | - |
9H-carbazole | mutant enzyme Q282N, at pH 7.5 and 30°C | Janthinobacterium sp. J3 | |
1.14.12.22 | 4.62 | - |
9H-carbazole | mutant enzyme I262V, at pH 7.5 and 30°C | Janthinobacterium sp. J3 | |
1.14.12.22 | 5.49 | - |
9H-carbazole | mutant enzyme Q282Y, at pH 7.5 and 30°C | Janthinobacterium sp. J3 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.12.22 | NADH | - |
Janthinobacterium sp. J3 | |
1.14.12.22 | NADPH | - |
Janthinobacterium sp. J3 | |
1.14.12.22 | [2Fe-2S]-center | - |
Janthinobacterium sp. J3 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.12.22 | 0.000733 | - |
9H-carbazole | mutant enzyme Q282N, at pH 7.5 and 30°C | Janthinobacterium sp. J3 | |
1.14.12.22 | 0.00091 | - |
9H-carbazole | mutant enzyme I262V, at pH 7.5 and 30°C | Janthinobacterium sp. J3 | |
1.14.12.22 | 0.001 | - |
9H-carbazole | mutant enzyme Q282Y, at pH 7.5 and 30°C | Janthinobacterium sp. J3 |