Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Meleiro, L.; Carli, S.; Fonseca-Maldonado, R.; da Silva Torricillas, M.; Zimbardi, A.; Ward, R.; Jorge, J.; Furriel, R.
    Overexpression of a cellobiose-glucose-halotolerant endoglucanase from Scytalidium thermophilum (2017), Appl. Biochem. Biotechnol., 185, 316-333.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.4 2-mercaptoethanol 10 mM, 1.8fold stimulation Mycothermus thermophilus
3.2.1.4 cysteine 10 mM, 1.6fold stimulation Mycothermus thermophilus
3.2.1.4 dithiothreitol 10 mM, 1.8fold stimulation Mycothermus thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Pichia pastoris Mycothermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Co2+ 5 mM, 76% residual activity Mycothermus thermophilus
3.2.1.4 Hg2+ 1 mM, no residual activity Mycothermus thermophilus
3.2.1.4 additional information enzyme retains 84.1 and 71.4% of the control activity in the presence of 0.5 and 2.0 mol/l NaCl, respectively Mycothermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 carboxymethyl cellulose Km value in absence of Mn2+ 10.5 mg/ml, in presence of Mn2+ 7.8 mg/ml, pH 5.5, 60°C Mycothermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Manganese 5 mM, 1.8fold stimulation Mycothermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Mycothermus thermophilus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.4 glycoprotein 53% carbohydrate content, molecular mass of the Endo H-treated enzyme is approximately 42 kDa Mycothermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 0.89
-
 substrate filter paper, pH 5.5, 60°C Mycothermus thermophilus
3.2.1.4 192
-
 substrate medium viscosity carboxymethyl cellulose, pH 5.5, 60°C Mycothermus thermophilus
3.2.1.4 246
-
 substrate low viscosity carboxymethyl cellulose, pH 5.5, 60°C Mycothermus thermophilus
3.2.1.4 248
-
 substrate barley beta-glucan, pH 5.5, 60°C Mycothermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 barley beta-glucan + H2O
-
 Mycothermus thermophilus ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O medium and low viscosity Mycothermus thermophilus ?
-
 ?
3.2.1.4 filter paper + H2O
-
 Mycothermus thermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 monomer 1 * 66000, SDS-PAGE, 1 * 42000, SDS-PAGE of deglycosylated protein Mycothermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 60 70
-
 Mycothermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 65
-
 half-life 100 min Mycothermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.4 475
-
 carboxymethyl cellulose absence of Mn2+, pH 5.5, 60°C Mycothermus thermophilus
3.2.1.4 677
-
 carboxymethyl cellulose presence of Mn2+, pH 5.5, 60°C Mycothermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5.5
-
-
 Mycothermus thermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 3 8
-
 Mycothermus thermophilus