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Literature summary extracted from
- Cloning and expression of the limonene hydroxylase of Bacillus stearothermophilus BR388 and utilization in two-phase limonene conversions (2000), Appl. Biochem. Biotechnol., 84-86, 903-915 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.51 | expressed in Escherichia coli BL21(DE3) cells | Geobacillus stearothermophilus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.51 | (S)-limonene + [reduced NADH-hemoprotein reductase] + O2 | Geobacillus stearothermophilus | - |
(-)-trans-carveol + [oxidized NADH-hemoprotein reductase] + H2O | - |
? |  |
| 1.14.14.51 | (S)-limonene + [reduced NADH-hemoprotein reductase] + O2 | Geobacillus stearothermophilus BR388 | - |
(-)-trans-carveol + [oxidized NADH-hemoprotein reductase] + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.51 | Geobacillus stearothermophilus | O85057 | - |
- |
| 1.14.14.51 | Geobacillus stearothermophilus BR388 | O85057 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.51 | (S)-limonene + [reduced NADH-hemoprotein reductase] + O2 | - |
Geobacillus stearothermophilus | (-)-trans-carveol + [oxidized NADH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.51 | (S)-limonene + [reduced NADH-hemoprotein reductase] + O2 | - |
Geobacillus stearothermophilus BR388 | (-)-trans-carveol + [oxidized NADH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.51 | 1-cyclohexene 1-methanol + NAD+ | 9.6% activity compared to perillyl alcohol | Geobacillus stearothermophilus | 1-cyclohexen-1-carbaldehyde + NADH + H+ | - |
? | |
| 1.14.14.51 | 1-cyclohexene 1-methanol + NAD+ | 9.6% activity compared to perillyl alcohol | Geobacillus stearothermophilus BR388 | 1-cyclohexen-1-carbaldehyde + NADH + H+ | - |
? | |
| 1.14.14.51 | 4-isopropyl benzyl alcohol + NAD+ | 42.2% activity compared to perillyl alcohol | Geobacillus stearothermophilus | 4-isopropylbenzyl aldehyde + NADH | - |
? | |
| 1.14.14.51 | 4-isopropyl benzyl alcohol + NAD+ | 42.2% activity compared to perillyl alcohol | Geobacillus stearothermophilus BR388 | 4-isopropylbenzyl aldehyde + NADH | - |
? | |
| 1.14.14.51 | 4-methyl benzyl alcohol + NAD+ | 17.1% activity compared to perillyl alcohol | Geobacillus stearothermophilus | ? + NADH | - |
? | |
| 1.14.14.51 | benzyl alcohol + NAD+ | 3.1% activity compared to perillyl alcohol | Geobacillus stearothermophilus | benzaldehyde + NADH | - |
? | |
| 1.14.14.51 | benzyl alcohol + NAD+ | 3.1% activity compared to perillyl alcohol | Geobacillus stearothermophilus BR388 | benzaldehyde + NADH | - |
? | |
| 1.14.14.51 | carveol + NAD+ | 86% activity compared to perillyl alcohol | Geobacillus stearothermophilus | ? + NADH | - |
? | |
| 1.14.14.51 | cyclohexyl methanol + NAD+ | 15.8% activity compared to perillyl alcohol | Geobacillus stearothermophilus | cyclohexylaldehyde + NADH | - |
? | |
| 1.14.14.51 | myrtenol + NAD+ | 4.0% activity compared to perillyl alcohol | Geobacillus stearothermophilus | myrtenal + NADH | - |
? | |
| 1.14.14.51 | perillyl alcohol + NAD+ | 100% activity | Geobacillus stearothermophilus | perillyl aldehyde + NADH | - |
r | |
| 1.14.14.51 | perillyl alcohol + NAD+ | 100% activity | Geobacillus stearothermophilus BR388 | perillyl aldehyde + NADH | - |
r | |
| 1.14.14.51 | perillyl aldehyde + NADH | - |
Geobacillus stearothermophilus | perillyl alcohol + NAD+ | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.51 | limonene hydroxylase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.51 | 45 | - |
- |
Geobacillus stearothermophilus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.51 | 30 | 55 | about 35% activity at 30°C, about 55% activity at 37°C, about 90% activity at 40°C, 100% activity at 45°C, about 75% activity at 50°C, about 30% activity at 55°C | Geobacillus stearothermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.51 | 7.7 | - |
- |
Geobacillus stearothermophilus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.51 | 5.5 | 10 | about 40% activity at pH 5.5, about 75% activity at pH 6.5, about 90% activity at pH 7.0, 100% activity at 8.0, about 75% activity at pH 9.0, about 40% activity at pH 10.0 | Geobacillus stearothermophilus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.51 | FAD | stimulates the hydroxylation reaction | Geobacillus stearothermophilus | |
| 1.14.14.51 | NAD+ | stimulates the dehydrogenase reaction | Geobacillus stearothermophilus | |
| 1.14.14.51 | NADH | stimulates the hydroxylation reaction | Geobacillus stearothermophilus | |
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