Literature summary extracted from
Zhang, L.S.; Fang, Y.; Zhou, Y.; Ye, B.C.
Improvement of the Stabilization and activity of protocatechuate 3,4-dioxygenase isolated from Rhizobium sp. LMB-1 and immobilized on Fe3O4 nanoparticles (2017), Appl. Biochem. Biotechnol., 183, 1035-1048 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.13.11.3 |
expressed in Escherichia coli BL21(DE3) cells |
Rhizobium sp. LMB-1 |
General Stability
EC Number |
General Stability |
Organism |
---|
1.13.11.3 |
Fe3O4 nanoparticles-immobilized enzyme exhibits improved thermal stability compared to the free enzyme |
Rhizobium sp. LMB-1 |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.13.11.3 |
Al3+ |
23.1% residual activity at 1 mM |
Rhizobium sp. LMB-1 |
|
1.13.11.3 |
butanol |
about 75% residual activity at 100 mM |
Rhizobium sp. LMB-1 |
|
1.13.11.3 |
ethanol |
about 50% residual activity at 100 mM |
Rhizobium sp. LMB-1 |
|
1.13.11.3 |
K+ |
16.7% residual activity at 1 mM |
Rhizobium sp. LMB-1 |
|
1.13.11.3 |
methanol |
about 75% residual activity at 100 mM |
Rhizobium sp. LMB-1 |
|
1.13.11.3 |
Mg2+ |
50% residual activity at 1 mM |
Rhizobium sp. LMB-1 |
|
1.13.11.3 |
Mn2+ |
45.2% residual activity at 1 mM |
Rhizobium sp. LMB-1 |
|
1.13.11.3 |
Ni2+ |
38.3% residual activity at 1 mM |
Rhizobium sp. LMB-1 |
|
1.13.11.3 |
Propanol |
about 40% residual activity at 100 mM |
Rhizobium sp. LMB-1 |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
1.13.11.3 |
0.06534 |
- |
3,4-dihydroxybenzoate |
free enzyme, at pH 7.4 and 37°C |
Rhizobium sp. LMB-1 |
|
1.13.11.3 |
0.15929 |
- |
3,4-dihydroxybenzoate |
Fe3O4 nanoparticles-immobilized enzyme, at pH 7.4 and 37°C |
Rhizobium sp. LMB-1 |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.13.11.3 |
Fe3+ |
115% activity at 1 mM |
Rhizobium sp. LMB-1 |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.13.11.3 |
500000 |
- |
gel filtration |
Rhizobium sp. LMB-1 |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.13.11.3 |
3,4-dihydroxybenzoate + O2 |
Rhizobium sp. LMB-1 |
- |
3-carboxy-cis,cis-muconate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.13.11.3 |
Rhizobium sp. LMB-1 |
A0A193DXA9 and A0A193DXP2 |
subunit alpha and subunit beta |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.13.11.3 |
Ni-affinity column chromatography |
Rhizobium sp. LMB-1 |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.13.11.3 |
3,4-dihydroxybenzoate + O2 |
- |
Rhizobium sp. LMB-1 |
3-carboxy-cis,cis-muconate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.13.11.3 |
heterodimer |
1 * 23700 + 1 * 28600, SDS-PAGE |
Rhizobium sp. LMB-1 |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.13.11.3 |
P34O |
- |
Rhizobium sp. LMB-1 |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.13.11.3 |
60 |
- |
- |
Rhizobium sp. LMB-1 |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
1.13.11.3 |
20 |
70 |
the enzyme shows more than 50% activity between 20 and 70°C |
Rhizobium sp. LMB-1 |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
1.13.11.3 |
60 |
70 |
after incubating for 330 min at 60°C, the free enzyme maintains approximately 17% of its activity, and its half-life is 180 min. The enzyme retains 75% activity after 4 h at 70°C and shows no activity after 4 h at 90°C |
Rhizobium sp. LMB-1 |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
1.13.11.3 |
10.25 |
- |
3,4-dihydroxybenzoate |
free enzyme, at pH 7.4 and 37°C |
Rhizobium sp. LMB-1 |
|
1.13.11.3 |
15.97 |
- |
3,4-dihydroxybenzoate |
Fe3O4 nanoparticles-immobilized enzyme, at pH 7.4 and 37°C |
Rhizobium sp. LMB-1 |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.13.11.3 |
7.5 |
- |
- |
Rhizobium sp. LMB-1 |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
1.13.11.3 |
5.5 |
8.5 |
the enzyme shows more than 50% activity between pH 5.5 and 8.5 |
Rhizobium sp. LMB-1 |