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Literature summary extracted from

  • Zhang, L.S.; Fang, Y.; Zhou, Y.; Ye, B.C.
    Improvement of the Stabilization and activity of protocatechuate 3,4-dioxygenase isolated from Rhizobium sp. LMB-1 and immobilized on Fe3O4 nanoparticles (2017), Appl. Biochem. Biotechnol., 183, 1035-1048 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.3 expressed in Escherichia coli BL21(DE3) cells Rhizobium sp. LMB-1

General Stability

EC Number General Stability Organism
1.13.11.3 Fe3O4 nanoparticles-immobilized enzyme exhibits improved thermal stability compared to the free enzyme Rhizobium sp. LMB-1

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.3 Al3+ 23.1% residual activity at 1 mM Rhizobium sp. LMB-1
1.13.11.3 butanol about 75% residual activity at 100 mM Rhizobium sp. LMB-1
1.13.11.3 ethanol about 50% residual activity at 100 mM Rhizobium sp. LMB-1
1.13.11.3 K+ 16.7% residual activity at 1 mM Rhizobium sp. LMB-1
1.13.11.3 methanol about 75% residual activity at 100 mM Rhizobium sp. LMB-1
1.13.11.3 Mg2+ 50% residual activity at 1 mM Rhizobium sp. LMB-1
1.13.11.3 Mn2+ 45.2% residual activity at 1 mM Rhizobium sp. LMB-1
1.13.11.3 Ni2+ 38.3% residual activity at 1 mM Rhizobium sp. LMB-1
1.13.11.3 Propanol about 40% residual activity at 100 mM Rhizobium sp. LMB-1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.3 0.06534
-
3,4-dihydroxybenzoate free enzyme, at pH 7.4 and 37°C Rhizobium sp. LMB-1
1.13.11.3 0.15929
-
3,4-dihydroxybenzoate Fe3O4 nanoparticles-immobilized enzyme, at pH 7.4 and 37°C Rhizobium sp. LMB-1

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.3 Fe3+ 115% activity at 1 mM Rhizobium sp. LMB-1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.3 500000
-
gel filtration Rhizobium sp. LMB-1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.3 3,4-dihydroxybenzoate + O2 Rhizobium sp. LMB-1
-
3-carboxy-cis,cis-muconate
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.3 Rhizobium sp. LMB-1 A0A193DXA9 and A0A193DXP2 subunit alpha and subunit beta
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.3 Ni-affinity column chromatography Rhizobium sp. LMB-1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.3 3,4-dihydroxybenzoate + O2
-
Rhizobium sp. LMB-1 3-carboxy-cis,cis-muconate
-
?

Subunits

EC Number Subunits Comment Organism
1.13.11.3 heterodimer 1 * 23700 + 1 * 28600, SDS-PAGE Rhizobium sp. LMB-1

Synonyms

EC Number Synonyms Comment Organism
1.13.11.3 P34O
-
Rhizobium sp. LMB-1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.3 60
-
-
Rhizobium sp. LMB-1

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.3 20 70 the enzyme shows more than 50% activity between 20 and 70°C Rhizobium sp. LMB-1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.3 60 70 after incubating for 330 min at 60°C, the free enzyme maintains approximately 17% of its activity, and its half-life is 180 min. The enzyme retains 75% activity after 4 h at 70°C and shows no activity after 4 h at 90°C Rhizobium sp. LMB-1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.3 10.25
-
3,4-dihydroxybenzoate free enzyme, at pH 7.4 and 37°C Rhizobium sp. LMB-1
1.13.11.3 15.97
-
3,4-dihydroxybenzoate Fe3O4 nanoparticles-immobilized enzyme, at pH 7.4 and 37°C Rhizobium sp. LMB-1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.3 7.5
-
-
Rhizobium sp. LMB-1

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.3 5.5 8.5 the enzyme shows more than 50% activity between pH 5.5 and 8.5 Rhizobium sp. LMB-1