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Literature summary extracted from

  • Singh, R.; Trivedi, V.D.; Phale, P.S.
    Purification and characterization of NAD+-dependent salicylaldehyde dehydrogenase from carbaryl-degrading Pseudomonas sp. strain C6 (2014), Appl. Biochem. Biotechnol., 172, 806-819 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.65 1,10-phenanthroline 40% inhibition at 1 mM Pseudomonas sp.
1.2.1.65 2,2'-dipyridyl 50% inhibition at 1 mM Pseudomonas sp.
1.2.1.65 2-naphthaldehyde substrate inhibition Pseudomonas sp.
1.2.1.65 3-Chlorobenzaldehyde substrate inhibition Pseudomonas sp.
1.2.1.65 3-Hydroxybenzaldehyde substrate inhibition Pseudomonas sp.
1.2.1.65 4-Chlorobenzaldehyde substrate inhibition Pseudomonas sp.
1.2.1.65 4-nitrobenzaldehyde substrate inhibition Pseudomonas sp.
1.2.1.65 benzaldehyde substrate inhibition Pseudomonas sp.
1.2.1.65 EDTA 50% inhibition at 50 mM Pseudomonas sp.
1.2.1.65 EGTA 60% inhibition at 50 mM Pseudomonas sp.
1.2.1.65 Na+ inhibitory at higher concentration Pseudomonas sp.
1.2.1.65 Salicylaldehyde substrate inhibition, 86% and 70% decrease in activity at 0.05 mM and 0.1 mM, respectively Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.65 additional information
-
 additional information Michaelis-Menten kinetics for NAD+ Pseudomonas sp.
1.2.1.65 0.001
-
 benzaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 0.0021
-
 3-Hydroxybenzaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 0.0035
-
 2-naphthaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 0.0039
-
 3-Chlorobenzaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 0.0045
-
 Salicylaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 0.0064
-
 4-Chlorobenzaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 0.011
-
 4-nitrobenzaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 0.052
-
 NAD+ pH 8.0, temperature not specified in the publication Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.65 Ca2+ the enzyme contains 1.8 mol/mol of enzyme Pseudomonas sp.
1.2.1.65 Fe2+ the enzyme contains 0.4 mol/mol of enzyme Pseudomonas sp.
1.2.1.65 K+ 44% activating at 200 mM Pseudomonas sp.
1.2.1.65 Mg2+ the enzyme contains 0.3 mol/mol of enzyme Pseudomonas sp.
1.2.1.65 additional information external addition of Ca2+, Mg2+, Fe2+ and Fe3+ at 10 mM, either as sulfate or chloride salts, fail to show an increase in the enzyme activity Pseudomonas sp.
1.2.1.65 Zn2+ the enzyme contains 0.2 mol/mol of enzyme Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.65 150000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.65 salicylaldehyde + NAD+ + H2O Pseudomonas sp.
-
 salicylate + NADH + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.65 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.65 native enzyme 28fold from cellfree extract by anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography, and gel filtration Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.65 36.3
-
 purified native enzyme, pH 8.0, temperature not specified in the publication Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.65 1-naphthaldehyde + NAD+ + H2O low activity Pseudomonas sp. 1-naphthoate + NADH + 2 H+
-
 ?
1.2.1.65 2-carboxybenzaldehyde + NAD+ + H2O very low activity Pseudomonas sp. 2-carboxybenzoate + NADH + 2 H+
-
 ?
1.2.1.65 2-chlorobenzaldehyde + NAD+ + H2O low activity Pseudomonas sp. 2-chlorobenzoate + NADH + 2 H+
-
 ?
1.2.1.65 2-methoxybenzaldehyde + NAD+ + H2O low activity Pseudomonas sp. 2-methoxybenzoate + NADH + 2 H+
-
 ?
1.2.1.65 2-naphthaldehyde + NAD+ + H2O moderate activity Pseudomonas sp. 2-naphthoate + NADH + 2 H+
-
 ?
1.2.1.65 2-nitrobenzaldehyde + NAD+ + H2O low activity Pseudomonas sp. 2-nitrobenzoate + NADH + 2 H+
-
 ?
1.2.1.65 3-carboxybenzaldehyde + NAD+ + H2O very low activity Pseudomonas sp. 3-carboxybenzoate + NADH + 2 H+
-
 ?
1.2.1.65 3-chlorobenzaldehyde + NAD+ + H2O moderate activity Pseudomonas sp. 3-chlorobenzoate + NADH + 2 H+
-
 ?
1.2.1.65 3-hydroxybenzaldehyde + NAD+ + H2O low to moderate activity Pseudomonas sp. 3-hydroxybenzoate + NADH + 2 H+
-
 ?
1.2.1.65 3-methoxybenzaldehyde + NAD+ + H2O moderate activity Pseudomonas sp. 3-methoxybenzoate + NADH + 2 H+
-
 ?
1.2.1.65 3-nitrobenzaldehyde + NAD+ + H2O low activity Pseudomonas sp. 3-nitrobenzoate + NADH + 2 H+
-
 ?
1.2.1.65 4-carboxybenzaldehyde + NAD+ + H2O very low activity Pseudomonas sp. 4-carboxybenzoate + NADH + 2 H+
-
 ?
1.2.1.65 4-chlorobenzaldehyde + NAD+ + H2O high activity Pseudomonas sp. 4-chlorobenzoate + NADH + 2 H+
-
 ?
1.2.1.65 4-hydroxybenzaldehyde + NAD+ + H2O low activity Pseudomonas sp. 4-hydroxybenzoate + NADH + 2 H+
-
 ?
1.2.1.65 4-methoxybenzaldehyde + NAD+ + H2O low activity Pseudomonas sp. 4-methoxybenzoate + NADH + 2 H+
-
 ?
1.2.1.65 4-nitrobenzaldehyde + NAD+ + H2O moderate activity Pseudomonas sp. 4-nitrobenzoate + NADH + 2 H+
-
 ?
1.2.1.65 acetaldehyde + NAD+ + H2O very low activity Pseudomonas sp. acetate + NADH + 2 H+
-
 ?
1.2.1.65 benzaldehyde + NAD+ + H2O moderate to high activity Pseudomonas sp. benzoate + NADH + 2 H+
-
 ?
1.2.1.65 butyraldehyde + NAD+ + H2O low activity Pseudomonas sp. butyrate + NADH + 2 H+
-
 ?
1.2.1.65 formaldehyde + NAD+ + H2O very low activity Pseudomonas sp. formate + NADH + 2 H+
-
 ?
1.2.1.65 glutaraldehyde + NAD+ + H2O very low activity Pseudomonas sp. glutarate + NADH + 2 H+
-
 ?
1.2.1.65 isovaleraldehyde + NAD+ + H2O very low activity Pseudomonas sp. isovalerate + NADH + 2 H+
-
 ?
1.2.1.65 additional information the enzyme shows maximum activity on salicylaldehyde and very poor activity on aliphatic aldehydes. The preferences of substrate (derivatives of benzaldehyde) for enzyme SALDH are dependent on nature, position and group of substituents. Enzyme SALDH prefers: (1) hydroxyl derivative at ortho and meta position, (2) chloro and nitro derivative at meta and para position, and (3) methoxy at meta position. The carboxyl group substitution is poorly preferred. Enzyme SALDH also shows activity on di-aromatic aldehydes. Substrate specificity, overview Pseudomonas sp. ?
-
 ?
1.2.1.65 propionaldehyde + NAD+ + H2O very low activity Pseudomonas sp. propionate + NADH + 2 H+
-
 ?
1.2.1.65 salicylaldehyde + NAD+ + H2O
-
 Pseudomonas sp. salicylate + NADH + 2 H+
-
 ?
1.2.1.65 salicylaldehyde + NAD+ + H2O best substrate Pseudomonas sp. salicylate + NADH + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.65 homotrimer 3 * 50000, SDS-PAGE Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.2.1.65 NAD+-dependent salicylaldehyde dehydrogenase
-
 Pseudomonas sp.
1.2.1.65 sALDH
-
 Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.65 18
-
 3-Hydroxybenzaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 26.4
-
 benzaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 59
-
 4-nitrobenzaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 65
-
 3-Chlorobenzaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 93.6
-
 Salicylaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 96
-
 2-naphthaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 98
-
 4-Chlorobenzaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.65 8
-
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.65 additional information enzyme SALDH shows poor activity with NADP+ Pseudomonas sp.
1.2.1.65 NAD+ dependent on Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.65 0.0075
-
 3-Chlorobenzaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 0.0773
-
 4-Chlorobenzaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 0.126
-
 4-nitrobenzaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 0.136
-
 2-naphthaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 0.185
-
 Salicylaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 0.306
-
 benzaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 1.364
-
 3-Hydroxybenzaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.

General Information

EC Number General Information Comment Organism
1.2.1.65 physiological function the enzyme catalyzes the oxidation of salicylaldehyde to salicylate in the carbaryl-degrading Pseudomonas sp. strain C6 Pseudomonas sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.65 6000
-
 4-nitrobenzaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 8800
-
 3-Hydroxybenzaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 12400
-
 4-Chlorobenzaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 21500
-
 3-Chlorobenzaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 23000
-
 Salicylaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 26000
-
 benzaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.
1.2.1.65 29000
-
 2-naphthaldehyde pH 8.0, temperature not specified in the publication Pseudomonas sp.